Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12620

Experiment: KLEB1 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12615 and BWI76_RS12620 are separated by 158 nucleotidesBWI76_RS12620 and BWI76_RS12625 are separated by 102 nucleotides BWI76_RS12615: BWI76_RS12615 - LysR family transcriptional regulator, at 2,632,949 to 2,633,860 _RS12615 BWI76_RS12620: BWI76_RS12620 - 2-nitropropane dioxygenase, at 2,634,019 to 2,634,999 _RS12620 BWI76_RS12625: BWI76_RS12625 - isoflavone reductase, at 2,635,102 to 2,636,031 _RS12625 Position (kb) 2634 2635Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2633.143 kb on - strand, within BWI76_RS12615at 2633.145 kb on + strand, within BWI76_RS12615at 2633.596 kb on + strand, within BWI76_RS12615at 2633.609 kb on - strand, within BWI76_RS12615at 2633.721 kb on + strand, within BWI76_RS12615at 2633.726 kb on + strand, within BWI76_RS12615at 2633.729 kb on - strand, within BWI76_RS12615at 2633.954 kb on + strandat 2633.962 kb on - strandat 2634.002 kb on - strandat 2634.265 kb on - strand, within BWI76_RS12620at 2634.265 kb on - strand, within BWI76_RS12620at 2634.347 kb on + strand, within BWI76_RS12620at 2634.382 kb on - strand, within BWI76_RS12620at 2634.382 kb on - strand, within BWI76_RS12620at 2634.382 kb on - strand, within BWI76_RS12620at 2634.392 kb on - strand, within BWI76_RS12620at 2634.621 kb on + strand, within BWI76_RS12620at 2634.907 kb on + strandat 2635.013 kb on - strandat 2635.044 kb on + strandat 2635.131 kb on - strandat 2635.194 kb on - strandat 2635.196 kb on - strand, within BWI76_RS12625at 2635.270 kb on - strand, within BWI76_RS12625at 2635.287 kb on + strand, within BWI76_RS12625at 2635.319 kb on - strand, within BWI76_RS12625at 2635.365 kb on + strand, within BWI76_RS12625at 2635.425 kb on - strand, within BWI76_RS12625at 2635.438 kb on - strand, within BWI76_RS12625at 2635.558 kb on + strand, within BWI76_RS12625at 2635.698 kb on - strand, within BWI76_RS12625at 2635.709 kb on + strand, within BWI76_RS12625

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1
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2,633,143 - BWI76_RS12615 0.21 +0.0
2,633,145 + BWI76_RS12615 0.21 +0.5
2,633,596 + BWI76_RS12615 0.71 -0.4
2,633,609 - BWI76_RS12615 0.72 -0.0
2,633,721 + BWI76_RS12615 0.85 -0.3
2,633,726 + BWI76_RS12615 0.85 -1.2
2,633,729 - BWI76_RS12615 0.86 -1.6
2,633,954 + -1.1
2,633,962 - -2.5
2,634,002 - -3.1
2,634,265 - BWI76_RS12620 0.25 -2.8
2,634,265 - BWI76_RS12620 0.25 -0.4
2,634,347 + BWI76_RS12620 0.33 -1.2
2,634,382 - BWI76_RS12620 0.37 -0.1
2,634,382 - BWI76_RS12620 0.37 -2.6
2,634,382 - BWI76_RS12620 0.37 -3.0
2,634,392 - BWI76_RS12620 0.38 -2.3
2,634,621 + BWI76_RS12620 0.61 -3.1
2,634,907 + -1.2
2,635,013 - -0.4
2,635,044 + -0.4
2,635,131 - -0.9
2,635,194 - +0.5
2,635,196 - BWI76_RS12625 0.10 +2.2
2,635,270 - BWI76_RS12625 0.18 -0.5
2,635,287 + BWI76_RS12625 0.20 -1.5
2,635,319 - BWI76_RS12625 0.23 +0.8
2,635,365 + BWI76_RS12625 0.28 -1.0
2,635,425 - BWI76_RS12625 0.35 +0.1
2,635,438 - BWI76_RS12625 0.36 +0.8
2,635,558 + BWI76_RS12625 0.49 -0.1
2,635,698 - BWI76_RS12625 0.64 -0.7
2,635,709 + BWI76_RS12625 0.65 -2.4

Or see this region's nucleotide sequence