Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12370 and BWI76_RS12375 are separated by 91 nucleotides BWI76_RS12375 and BWI76_RS12380 are separated by 488 nucleotides BWI76_RS12380 and BWI76_RS12385 are separated by 1 nucleotides
BWI76_RS12370: BWI76_RS12370 - hypothetical protein, at 2,583,369 to 2,585,315
_RS12370
BWI76_RS12375: BWI76_RS12375 - DNA-invertase hin, at 2,585,407 to 2,585,958
_RS12375
BWI76_RS12380: BWI76_RS12380 - DNA polymerase V subunit UmuD, at 2,586,447 to 2,586,866
_RS12380
BWI76_RS12385: BWI76_RS12385 - DNA polymerase V subunit UmuC, at 2,586,868 to 2,588,133
_RS12385
Position (kb)
2585
2586 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2584.559 kb on - strand, within BWI76_RS12370 at 2584.563 kb on + strand, within BWI76_RS12370 at 2584.564 kb on - strand, within BWI76_RS12370 at 2584.773 kb on - strand, within BWI76_RS12370 at 2584.773 kb on - strand, within BWI76_RS12370 at 2584.965 kb on - strand, within BWI76_RS12370 at 2585.108 kb on + strand, within BWI76_RS12370 at 2585.211 kb on + strand at 2585.536 kb on + strand, within BWI76_RS12375 at 2585.614 kb on - strand, within BWI76_RS12375 at 2585.644 kb on - strand, within BWI76_RS12375 at 2585.800 kb on + strand, within BWI76_RS12375 at 2586.058 kb on - strand at 2586.071 kb on + strand at 2586.188 kb on - strand at 2586.460 kb on - strand at 2586.478 kb on + strand at 2586.505 kb on + strand, within BWI76_RS12380 at 2586.547 kb on - strand, within BWI76_RS12380 at 2586.570 kb on - strand, within BWI76_RS12380 at 2586.582 kb on + strand, within BWI76_RS12380 at 2586.687 kb on + strand, within BWI76_RS12380 at 2586.758 kb on + strand, within BWI76_RS12380 at 2586.764 kb on + strand, within BWI76_RS12380 at 2586.825 kb on - strand at 2586.827 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 2,584,559 - BWI76_RS12370 0.61 +0.2 2,584,563 + BWI76_RS12370 0.61 -3.1 2,584,564 - BWI76_RS12370 0.61 +0.8 2,584,773 - BWI76_RS12370 0.72 +1.6 2,584,773 - BWI76_RS12370 0.72 +0.1 2,584,965 - BWI76_RS12370 0.82 -1.3 2,585,108 + BWI76_RS12370 0.89 -3.8 2,585,211 + +0.6 2,585,536 + BWI76_RS12375 0.23 -0.9 2,585,614 - BWI76_RS12375 0.38 -1.4 2,585,644 - BWI76_RS12375 0.43 +0.4 2,585,800 + BWI76_RS12375 0.71 -2.3 2,586,058 - +0.4 2,586,071 + +0.6 2,586,188 - -0.9 2,586,460 - -2.5 2,586,478 + -1.8 2,586,505 + BWI76_RS12380 0.14 -1.2 2,586,547 - BWI76_RS12380 0.24 -3.1 2,586,570 - BWI76_RS12380 0.29 -0.3 2,586,582 + BWI76_RS12380 0.32 -1.3 2,586,687 + BWI76_RS12380 0.57 -0.7 2,586,758 + BWI76_RS12380 0.74 +0.7 2,586,764 + BWI76_RS12380 0.75 +0.2 2,586,825 - -1.6 2,586,827 + -0.8
Or see this region's nucleotide sequence