Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12055 and BWI76_RS12060 are separated by 13 nucleotides BWI76_RS12060 and BWI76_RS12065 are separated by 192 nucleotides BWI76_RS12065 and BWI76_RS12070 overlap by 1 nucleotides BWI76_RS12070 and BWI76_RS12075 are separated by 284 nucleotides
BWI76_RS12055: BWI76_RS12055 - DNA single-strand annealing protein RecT-like protein, at 2,533,071 to 2,534,180
_RS12055
BWI76_RS12060: BWI76_RS12060 - putative exodeoxyribonuclease VIII, at 2,534,194 to 2,536,983
_RS12060
BWI76_RS12065: BWI76_RS12065 - hypothetical protein, at 2,537,176 to 2,537,367
_RS12065
BWI76_RS12070: BWI76_RS12070 - hypothetical protein, at 2,537,367 to 2,537,561
_RS12070
BWI76_RS12075: BWI76_RS12075 - regulator, at 2,537,846 to 2,538,406
_RS12075
Position (kb)
2534
2535
2536
2537 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2533.276 kb on + strand, within BWI76_RS12055 at 2533.531 kb on - strand, within BWI76_RS12055 at 2533.662 kb on - strand, within BWI76_RS12055 at 2533.933 kb on + strand, within BWI76_RS12055 at 2534.271 kb on + strand at 2534.446 kb on + strand at 2534.641 kb on + strand, within BWI76_RS12060 at 2534.723 kb on + strand, within BWI76_RS12060 at 2534.906 kb on - strand, within BWI76_RS12060 at 2534.995 kb on + strand, within BWI76_RS12060 at 2535.185 kb on - strand, within BWI76_RS12060 at 2535.264 kb on - strand, within BWI76_RS12060 at 2535.376 kb on + strand, within BWI76_RS12060 at 2535.376 kb on + strand, within BWI76_RS12060 at 2535.413 kb on - strand, within BWI76_RS12060 at 2535.447 kb on + strand, within BWI76_RS12060 at 2535.576 kb on + strand at 2535.576 kb on + strand, within BWI76_RS12060 at 2535.584 kb on - strand, within BWI76_RS12060 at 2535.646 kb on - strand, within BWI76_RS12060 at 2535.719 kb on - strand, within BWI76_RS12060 at 2535.781 kb on - strand, within BWI76_RS12060 at 2535.788 kb on - strand, within BWI76_RS12060 at 2535.943 kb on + strand, within BWI76_RS12060 at 2535.963 kb on + strand, within BWI76_RS12060 at 2535.963 kb on + strand, within BWI76_RS12060 at 2535.963 kb on + strand at 2536.096 kb on + strand, within BWI76_RS12060 at 2536.225 kb on - strand, within BWI76_RS12060 at 2536.227 kb on - strand, within BWI76_RS12060 at 2536.240 kb on - strand, within BWI76_RS12060 at 2536.364 kb on - strand, within BWI76_RS12060 at 2536.370 kb on - strand, within BWI76_RS12060 at 2536.511 kb on + strand, within BWI76_RS12060 at 2536.519 kb on - strand, within BWI76_RS12060 at 2536.519 kb on - strand, within BWI76_RS12060 at 2536.565 kb on + strand, within BWI76_RS12060 at 2536.573 kb on - strand, within BWI76_RS12060 at 2536.573 kb on - strand, within BWI76_RS12060 at 2536.613 kb on - strand, within BWI76_RS12060 at 2536.620 kb on - strand, within BWI76_RS12060 at 2536.883 kb on + strand at 2536.958 kb on + strand at 2537.032 kb on + strand at 2537.040 kb on - strand at 2537.174 kb on + strand at 2537.179 kb on + strand at 2537.219 kb on + strand, within BWI76_RS12065 at 2537.242 kb on + strand, within BWI76_RS12065 at 2537.289 kb on + strand, within BWI76_RS12065 at 2537.295 kb on - strand, within BWI76_RS12065 at 2537.528 kb on - strand, within BWI76_RS12070 at 2537.559 kb on - strand at 2537.608 kb on + strand at 2537.697 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 2,533,276 + BWI76_RS12055 0.18 -1.9 2,533,531 - BWI76_RS12055 0.41 +0.4 2,533,662 - BWI76_RS12055 0.53 -2.7 2,533,933 + BWI76_RS12055 0.78 -2.0 2,534,271 + -0.4 2,534,446 + +0.5 2,534,641 + BWI76_RS12060 0.16 +0.0 2,534,723 + BWI76_RS12060 0.19 +0.4 2,534,906 - BWI76_RS12060 0.26 -0.6 2,534,995 + BWI76_RS12060 0.29 +0.6 2,535,185 - BWI76_RS12060 0.36 -0.7 2,535,264 - BWI76_RS12060 0.38 -2.1 2,535,376 + BWI76_RS12060 0.42 +0.3 2,535,376 + BWI76_RS12060 0.42 +0.5 2,535,413 - BWI76_RS12060 0.44 -0.7 2,535,447 + BWI76_RS12060 0.45 -0.0 2,535,576 + +0.6 2,535,576 + BWI76_RS12060 0.50 -0.8 2,535,584 - BWI76_RS12060 0.50 -2.1 2,535,646 - BWI76_RS12060 0.52 -1.2 2,535,719 - BWI76_RS12060 0.55 -0.3 2,535,781 - BWI76_RS12060 0.57 -1.0 2,535,788 - BWI76_RS12060 0.57 -3.0 2,535,943 + BWI76_RS12060 0.63 +0.3 2,535,963 + BWI76_RS12060 0.63 -1.9 2,535,963 + BWI76_RS12060 0.63 +0.5 2,535,963 + -1.2 2,536,096 + BWI76_RS12060 0.68 -0.2 2,536,225 - BWI76_RS12060 0.73 -4.1 2,536,227 - BWI76_RS12060 0.73 -1.7 2,536,240 - BWI76_RS12060 0.73 +0.6 2,536,364 - BWI76_RS12060 0.78 -0.8 2,536,370 - BWI76_RS12060 0.78 -3.9 2,536,511 + BWI76_RS12060 0.83 -0.5 2,536,519 - BWI76_RS12060 0.83 -1.4 2,536,519 - BWI76_RS12060 0.83 -0.1 2,536,565 + BWI76_RS12060 0.85 -2.2 2,536,573 - BWI76_RS12060 0.85 -1.5 2,536,573 - BWI76_RS12060 0.85 -0.1 2,536,613 - BWI76_RS12060 0.87 -0.0 2,536,620 - BWI76_RS12060 0.87 -1.1 2,536,883 + -0.3 2,536,958 + -1.4 2,537,032 + +0.0 2,537,040 - -0.9 2,537,174 + +0.1 2,537,179 + -0.8 2,537,219 + BWI76_RS12065 0.22 +0.4 2,537,242 + BWI76_RS12065 0.34 -0.6 2,537,289 + BWI76_RS12065 0.59 -0.2 2,537,295 - BWI76_RS12065 0.62 -1.2 2,537,528 - BWI76_RS12070 0.83 -3.0 2,537,559 - -1.2 2,537,608 + +0.5 2,537,697 + -0.5
Or see this region's nucleotide sequence