Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12060

Experiment: KLEB1 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12055 and BWI76_RS12060 are separated by 13 nucleotidesBWI76_RS12060 and BWI76_RS12065 are separated by 192 nucleotidesBWI76_RS12065 and BWI76_RS12070 overlap by 1 nucleotidesBWI76_RS12070 and BWI76_RS12075 are separated by 284 nucleotides BWI76_RS12055: BWI76_RS12055 - DNA single-strand annealing protein RecT-like protein, at 2,533,071 to 2,534,180 _RS12055 BWI76_RS12060: BWI76_RS12060 - putative exodeoxyribonuclease VIII, at 2,534,194 to 2,536,983 _RS12060 BWI76_RS12065: BWI76_RS12065 - hypothetical protein, at 2,537,176 to 2,537,367 _RS12065 BWI76_RS12070: BWI76_RS12070 - hypothetical protein, at 2,537,367 to 2,537,561 _RS12070 BWI76_RS12075: BWI76_RS12075 - regulator, at 2,537,846 to 2,538,406 _RS12075 Position (kb) 2534 2535 2536 2537Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2533.276 kb on + strand, within BWI76_RS12055at 2533.531 kb on - strand, within BWI76_RS12055at 2533.662 kb on - strand, within BWI76_RS12055at 2533.933 kb on + strand, within BWI76_RS12055at 2534.271 kb on + strandat 2534.446 kb on + strandat 2534.641 kb on + strand, within BWI76_RS12060at 2534.723 kb on + strand, within BWI76_RS12060at 2534.906 kb on - strand, within BWI76_RS12060at 2534.995 kb on + strand, within BWI76_RS12060at 2535.185 kb on - strand, within BWI76_RS12060at 2535.264 kb on - strand, within BWI76_RS12060at 2535.376 kb on + strand, within BWI76_RS12060at 2535.376 kb on + strand, within BWI76_RS12060at 2535.413 kb on - strand, within BWI76_RS12060at 2535.447 kb on + strand, within BWI76_RS12060at 2535.576 kb on + strandat 2535.576 kb on + strand, within BWI76_RS12060at 2535.584 kb on - strand, within BWI76_RS12060at 2535.646 kb on - strand, within BWI76_RS12060at 2535.719 kb on - strand, within BWI76_RS12060at 2535.781 kb on - strand, within BWI76_RS12060at 2535.788 kb on - strand, within BWI76_RS12060at 2535.943 kb on + strand, within BWI76_RS12060at 2535.963 kb on + strand, within BWI76_RS12060at 2535.963 kb on + strand, within BWI76_RS12060at 2535.963 kb on + strandat 2536.096 kb on + strand, within BWI76_RS12060at 2536.225 kb on - strand, within BWI76_RS12060at 2536.227 kb on - strand, within BWI76_RS12060at 2536.240 kb on - strand, within BWI76_RS12060at 2536.364 kb on - strand, within BWI76_RS12060at 2536.370 kb on - strand, within BWI76_RS12060at 2536.511 kb on + strand, within BWI76_RS12060at 2536.519 kb on - strand, within BWI76_RS12060at 2536.519 kb on - strand, within BWI76_RS12060at 2536.565 kb on + strand, within BWI76_RS12060at 2536.573 kb on - strand, within BWI76_RS12060at 2536.573 kb on - strand, within BWI76_RS12060at 2536.613 kb on - strand, within BWI76_RS12060at 2536.620 kb on - strand, within BWI76_RS12060at 2536.883 kb on + strandat 2536.958 kb on + strandat 2537.032 kb on + strandat 2537.040 kb on - strandat 2537.174 kb on + strandat 2537.179 kb on + strandat 2537.219 kb on + strand, within BWI76_RS12065at 2537.242 kb on + strand, within BWI76_RS12065at 2537.289 kb on + strand, within BWI76_RS12065at 2537.295 kb on - strand, within BWI76_RS12065at 2537.528 kb on - strand, within BWI76_RS12070at 2537.559 kb on - strandat 2537.608 kb on + strandat 2537.697 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1
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2,533,276 + BWI76_RS12055 0.18 -1.9
2,533,531 - BWI76_RS12055 0.41 +0.4
2,533,662 - BWI76_RS12055 0.53 -2.7
2,533,933 + BWI76_RS12055 0.78 -2.0
2,534,271 + -0.4
2,534,446 + +0.5
2,534,641 + BWI76_RS12060 0.16 +0.0
2,534,723 + BWI76_RS12060 0.19 +0.4
2,534,906 - BWI76_RS12060 0.26 -0.6
2,534,995 + BWI76_RS12060 0.29 +0.6
2,535,185 - BWI76_RS12060 0.36 -0.7
2,535,264 - BWI76_RS12060 0.38 -2.1
2,535,376 + BWI76_RS12060 0.42 +0.3
2,535,376 + BWI76_RS12060 0.42 +0.5
2,535,413 - BWI76_RS12060 0.44 -0.7
2,535,447 + BWI76_RS12060 0.45 -0.0
2,535,576 + +0.6
2,535,576 + BWI76_RS12060 0.50 -0.8
2,535,584 - BWI76_RS12060 0.50 -2.1
2,535,646 - BWI76_RS12060 0.52 -1.2
2,535,719 - BWI76_RS12060 0.55 -0.3
2,535,781 - BWI76_RS12060 0.57 -1.0
2,535,788 - BWI76_RS12060 0.57 -3.0
2,535,943 + BWI76_RS12060 0.63 +0.3
2,535,963 + BWI76_RS12060 0.63 -1.9
2,535,963 + BWI76_RS12060 0.63 +0.5
2,535,963 + -1.2
2,536,096 + BWI76_RS12060 0.68 -0.2
2,536,225 - BWI76_RS12060 0.73 -4.1
2,536,227 - BWI76_RS12060 0.73 -1.7
2,536,240 - BWI76_RS12060 0.73 +0.6
2,536,364 - BWI76_RS12060 0.78 -0.8
2,536,370 - BWI76_RS12060 0.78 -3.9
2,536,511 + BWI76_RS12060 0.83 -0.5
2,536,519 - BWI76_RS12060 0.83 -1.4
2,536,519 - BWI76_RS12060 0.83 -0.1
2,536,565 + BWI76_RS12060 0.85 -2.2
2,536,573 - BWI76_RS12060 0.85 -1.5
2,536,573 - BWI76_RS12060 0.85 -0.1
2,536,613 - BWI76_RS12060 0.87 -0.0
2,536,620 - BWI76_RS12060 0.87 -1.1
2,536,883 + -0.3
2,536,958 + -1.4
2,537,032 + +0.0
2,537,040 - -0.9
2,537,174 + +0.1
2,537,179 + -0.8
2,537,219 + BWI76_RS12065 0.22 +0.4
2,537,242 + BWI76_RS12065 0.34 -0.6
2,537,289 + BWI76_RS12065 0.59 -0.2
2,537,295 - BWI76_RS12065 0.62 -1.2
2,537,528 - BWI76_RS12070 0.83 -3.0
2,537,559 - -1.2
2,537,608 + +0.5
2,537,697 + -0.5

Or see this region's nucleotide sequence