Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11890 and BWI76_RS11895 are separated by 16 nucleotides BWI76_RS11895 and BWI76_RS11900 overlap by 29 nucleotides BWI76_RS11900 and BWI76_RS11905 overlap by 4 nucleotides BWI76_RS11905 and BWI76_RS11910 overlap by 6 nucleotides BWI76_RS11910 and BWI76_RS11915 overlap by 1 nucleotides
BWI76_RS11890: BWI76_RS11890 - glycosyl hydrolase, at 2,501,942 to 2,504,299
_RS11890
BWI76_RS11895: BWI76_RS11895 - AraC family transcriptional regulator, at 2,504,316 to 2,505,290
_RS11895
BWI76_RS11900: BWI76_RS11900 - cob(I)yrinic acid a,c-diamide adenosyltransferase, at 2,505,262 to 2,505,852
_RS11900
BWI76_RS11905: BWI76_RS11905 - NAD(P)-dependent oxidoreductase, at 2,505,849 to 2,506,610
_RS11905
BWI76_RS11910: BWI76_RS11910 - hypothetical protein, at 2,506,605 to 2,506,820
_RS11910
BWI76_RS11915: BWI76_RS11915 - protease SohB, at 2,506,820 to 2,507,911
_RS11915
Position (kb)
2505
2506 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2504.550 kb on - strand, within BWI76_RS11895 at 2504.629 kb on - strand, within BWI76_RS11895 at 2505.106 kb on - strand, within BWI76_RS11895 at 2505.235 kb on + strand at 2505.235 kb on + strand at 2505.322 kb on - strand, within BWI76_RS11900 at 2505.820 kb on + strand at 2506.228 kb on - strand, within BWI76_RS11905 at 2506.821 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 2,504,550 - BWI76_RS11895 0.24 -2.9 2,504,629 - BWI76_RS11895 0.32 +1.3 2,505,106 - BWI76_RS11895 0.81 -0.1 2,505,235 + -0.1 2,505,235 + +0.9 2,505,322 - BWI76_RS11900 0.10 -2.0 2,505,820 + +2.6 2,506,228 - BWI76_RS11905 0.50 -3.0 2,506,821 + -1.1
Or see this region's nucleotide sequence