Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11395 and BWI76_RS11400 are separated by 180 nucleotides BWI76_RS11400 and BWI76_RS11405 are separated by 542 nucleotides BWI76_RS11405 and BWI76_RS11410 are separated by 88 nucleotides BWI76_RS11410 and BWI76_RS11415 are separated by 128 nucleotides
BWI76_RS11395: BWI76_RS11395 - RNA polymerase sigma factor FecI, at 2,402,426 to 2,402,947
_RS11395
BWI76_RS11400: BWI76_RS11400 - SAM-dependent methyltransferase, at 2,403,128 to 2,403,280
_RS11400
BWI76_RS11405: BWI76_RS11405 - lipid A biosynthesis acyltransferase, at 2,403,823 to 2,404,737
_RS11405
BWI76_RS11410: BWI76_RS11410 - leucine efflux protein, at 2,404,826 to 2,405,464
_RS11410
BWI76_RS11415: BWI76_RS11415 - hypothetical protein, at 2,405,593 to 2,405,856
_RS11415
Position (kb)
2403
2404
2405 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2402.862 kb on - strand, within BWI76_RS11395 at 2402.882 kb on + strand, within BWI76_RS11395 at 2402.890 kb on - strand, within BWI76_RS11395 at 2402.925 kb on - strand at 2403.133 kb on + strand at 2403.155 kb on + strand, within BWI76_RS11400 at 2403.163 kb on - strand, within BWI76_RS11400 at 2403.165 kb on - strand, within BWI76_RS11400 at 2403.165 kb on - strand, within BWI76_RS11400 at 2403.188 kb on + strand, within BWI76_RS11400 at 2403.238 kb on + strand, within BWI76_RS11400 at 2403.238 kb on + strand, within BWI76_RS11400 at 2403.238 kb on + strand, within BWI76_RS11400 at 2403.238 kb on + strand, within BWI76_RS11400 at 2403.248 kb on + strand, within BWI76_RS11400 at 2403.350 kb on + strand at 2403.404 kb on + strand at 2403.412 kb on - strand at 2403.421 kb on + strand at 2403.451 kb on + strand at 2403.451 kb on + strand at 2403.461 kb on + strand at 2403.480 kb on - strand at 2403.487 kb on + strand at 2403.487 kb on + strand at 2403.487 kb on + strand at 2403.487 kb on + strand at 2403.488 kb on + strand at 2403.509 kb on + strand at 2403.521 kb on + strand at 2403.530 kb on + strand at 2403.537 kb on - strand at 2403.539 kb on - strand at 2403.545 kb on + strand at 2403.545 kb on + strand at 2403.545 kb on + strand at 2403.553 kb on - strand at 2403.555 kb on + strand at 2403.555 kb on + strand at 2403.736 kb on + strand at 2403.736 kb on + strand at 2403.738 kb on + strand at 2403.744 kb on + strand at 2403.746 kb on - strand at 2403.751 kb on + strand at 2403.751 kb on + strand at 2403.759 kb on - strand at 2403.759 kb on - strand at 2403.759 kb on - strand at 2403.759 kb on - strand at 2403.761 kb on + strand at 2403.779 kb on - strand at 2403.793 kb on + strand at 2403.795 kb on - strand at 2403.797 kb on + strand at 2403.799 kb on + strand at 2403.799 kb on + strand at 2403.799 kb on + strand at 2403.858 kb on + strand at 2403.875 kb on + strand at 2403.875 kb on + strand at 2403.875 kb on + strand at 2403.875 kb on + strand at 2403.875 kb on + strand at 2403.875 kb on + strand at 2403.875 kb on + strand at 2403.875 kb on + strand at 2403.880 kb on + strand at 2403.881 kb on + strand at 2403.883 kb on - strand at 2403.883 kb on - strand at 2403.883 kb on - strand at 2403.883 kb on - strand at 2403.883 kb on - strand at 2403.885 kb on + strand at 2403.886 kb on + strand at 2403.886 kb on + strand at 2403.886 kb on + strand at 2403.886 kb on + strand at 2403.886 kb on + strand at 2403.886 kb on + strand at 2403.886 kb on + strand at 2403.886 kb on + strand at 2403.886 kb on + strand at 2403.886 kb on + strand at 2403.886 kb on + strand at 2403.891 kb on + strand at 2403.893 kb on - strand at 2403.894 kb on - strand at 2403.896 kb on + strand at 2403.907 kb on + strand at 2403.912 kb on + strand at 2403.920 kb on - strand, within BWI76_RS11405 at 2403.920 kb on - strand, within BWI76_RS11405 at 2403.920 kb on - strand, within BWI76_RS11405 at 2403.942 kb on - strand at 2403.968 kb on + strand, within BWI76_RS11405 at 2404.084 kb on - strand, within BWI76_RS11405 at 2404.107 kb on + strand, within BWI76_RS11405 at 2404.228 kb on - strand, within BWI76_RS11405 at 2404.246 kb on + strand, within BWI76_RS11405 at 2404.560 kb on + strand, within BWI76_RS11405 at 2404.562 kb on + strand, within BWI76_RS11405 at 2404.568 kb on - strand, within BWI76_RS11405 at 2404.570 kb on - strand, within BWI76_RS11405 at 2404.570 kb on - strand, within BWI76_RS11405 at 2404.570 kb on - strand, within BWI76_RS11405 at 2404.606 kb on - strand, within BWI76_RS11405 at 2404.609 kb on + strand, within BWI76_RS11405 at 2404.688 kb on - strand at 2404.777 kb on + strand at 2404.785 kb on - strand at 2404.877 kb on + strand at 2404.877 kb on + strand at 2405.126 kb on - strand, within BWI76_RS11410 at 2405.253 kb on - strand, within BWI76_RS11410 at 2405.461 kb on - strand at 2405.737 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 2,402,862 - BWI76_RS11395 0.84 +0.2 2,402,882 + BWI76_RS11395 0.87 -1.4 2,402,890 - BWI76_RS11395 0.89 +1.0 2,402,925 - +0.5 2,403,133 + -0.7 2,403,155 + BWI76_RS11400 0.18 +0.1 2,403,163 - BWI76_RS11400 0.23 +1.6 2,403,165 - BWI76_RS11400 0.24 -1.1 2,403,165 - BWI76_RS11400 0.24 -1.6 2,403,188 + BWI76_RS11400 0.39 +1.6 2,403,238 + BWI76_RS11400 0.72 -0.3 2,403,238 + BWI76_RS11400 0.72 +0.4 2,403,238 + BWI76_RS11400 0.72 +0.6 2,403,238 + BWI76_RS11400 0.72 -1.2 2,403,248 + BWI76_RS11400 0.78 +0.2 2,403,350 + +1.1 2,403,404 + -1.0 2,403,412 - +1.1 2,403,421 + +0.0 2,403,451 + -0.5 2,403,451 + +1.0 2,403,461 + -2.1 2,403,480 - -0.2 2,403,487 + -0.1 2,403,487 + -0.4 2,403,487 + +0.0 2,403,487 + +0.6 2,403,488 + -1.7 2,403,509 + -0.0 2,403,521 + -0.0 2,403,530 + -0.0 2,403,537 - +1.6 2,403,539 - +1.3 2,403,545 + +1.6 2,403,545 + +1.6 2,403,545 + -1.4 2,403,553 - -2.0 2,403,555 + -0.8 2,403,555 + -0.4 2,403,736 + +0.6 2,403,736 + -0.6 2,403,738 + +0.9 2,403,744 + +0.3 2,403,746 - -0.7 2,403,751 + -2.3 2,403,751 + +0.5 2,403,759 - -2.1 2,403,759 - -0.0 2,403,759 - -2.5 2,403,759 - +1.4 2,403,761 + -0.0 2,403,779 - +1.3 2,403,793 + +0.7 2,403,795 - -1.2 2,403,797 + +1.1 2,403,799 + -1.5 2,403,799 + -1.4 2,403,799 + -0.2 2,403,858 + +0.3 2,403,875 + -0.3 2,403,875 + +0.8 2,403,875 + +0.5 2,403,875 + -2.7 2,403,875 + -1.5 2,403,875 + +0.6 2,403,875 + -1.3 2,403,875 + -0.7 2,403,880 + +0.9 2,403,881 + -0.8 2,403,883 - -2.1 2,403,883 - -1.0 2,403,883 - -2.2 2,403,883 - -0.7 2,403,883 - -2.4 2,403,885 + +0.5 2,403,886 + -0.4 2,403,886 + +0.1 2,403,886 + -0.4 2,403,886 + -0.3 2,403,886 + -0.5 2,403,886 + -2.4 2,403,886 + -0.7 2,403,886 + -0.5 2,403,886 + +0.0 2,403,886 + -0.2 2,403,886 + -0.2 2,403,891 + -0.3 2,403,893 - -0.7 2,403,894 - -0.2 2,403,896 + +0.3 2,403,907 + +0.4 2,403,912 + -0.6 2,403,920 - BWI76_RS11405 0.11 +2.2 2,403,920 - BWI76_RS11405 0.11 +0.6 2,403,920 - BWI76_RS11405 0.11 +0.1 2,403,942 - +1.0 2,403,968 + BWI76_RS11405 0.16 +0.8 2,404,084 - BWI76_RS11405 0.29 -2.0 2,404,107 + BWI76_RS11405 0.31 -0.2 2,404,228 - BWI76_RS11405 0.44 +0.7 2,404,246 + BWI76_RS11405 0.46 -0.6 2,404,560 + BWI76_RS11405 0.81 -0.0 2,404,562 + BWI76_RS11405 0.81 -1.2 2,404,568 - BWI76_RS11405 0.81 -1.8 2,404,570 - BWI76_RS11405 0.82 -0.7 2,404,570 - BWI76_RS11405 0.82 -2.7 2,404,570 - BWI76_RS11405 0.82 +1.1 2,404,606 - BWI76_RS11405 0.86 -1.4 2,404,609 + BWI76_RS11405 0.86 +1.0 2,404,688 - +0.4 2,404,777 + -0.6 2,404,785 - -0.9 2,404,877 + -0.2 2,404,877 + -1.2 2,405,126 - BWI76_RS11410 0.47 -3.2 2,405,253 - BWI76_RS11410 0.67 -4.4 2,405,461 - -0.6 2,405,737 - +1.6
Or see this region's nucleotide sequence