Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10685 and BWI76_RS10690 overlap by 4 nucleotides BWI76_RS10690 and BWI76_RS10695 are separated by 2 nucleotides
BWI76_RS10685: BWI76_RS10685 - LysR family transcriptional regulator, at 2,257,894 to 2,258,850
_RS10685
BWI76_RS10690: BWI76_RS10690 - gamma-glutamylputrescine oxidoreductase, at 2,258,847 to 2,260,127
_RS10690
BWI76_RS10695: BWI76_RS10695 - aldehyde dehydrogenase PuuC, at 2,260,130 to 2,261,617
_RS10695
Position (kb)
2258
2259
2260
2261 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2257.928 kb on + strand at 2257.928 kb on + strand at 2258.359 kb on - strand, within BWI76_RS10685 at 2258.458 kb on + strand, within BWI76_RS10685 at 2258.466 kb on - strand, within BWI76_RS10685 at 2258.466 kb on - strand, within BWI76_RS10685 at 2258.466 kb on - strand, within BWI76_RS10685 at 2258.537 kb on + strand, within BWI76_RS10685 at 2259.014 kb on + strand, within BWI76_RS10690 at 2259.037 kb on + strand, within BWI76_RS10690 at 2259.456 kb on - strand, within BWI76_RS10690 at 2259.729 kb on - strand, within BWI76_RS10690 at 2259.731 kb on - strand, within BWI76_RS10690 at 2259.828 kb on + strand, within BWI76_RS10690 at 2259.909 kb on - strand, within BWI76_RS10690 at 2260.534 kb on - strand, within BWI76_RS10695 at 2260.554 kb on - strand, within BWI76_RS10695 at 2260.643 kb on - strand, within BWI76_RS10695 at 2260.715 kb on + strand, within BWI76_RS10695 at 2260.715 kb on + strand, within BWI76_RS10695 at 2260.771 kb on + strand, within BWI76_RS10695 at 2260.845 kb on - strand, within BWI76_RS10695 at 2261.044 kb on + strand, within BWI76_RS10695
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 2,257,928 + +0.5 2,257,928 + +0.7 2,258,359 - BWI76_RS10685 0.49 -0.2 2,258,458 + BWI76_RS10685 0.59 -0.3 2,258,466 - BWI76_RS10685 0.60 -0.8 2,258,466 - BWI76_RS10685 0.60 -0.1 2,258,466 - BWI76_RS10685 0.60 -4.4 2,258,537 + BWI76_RS10685 0.67 +0.6 2,259,014 + BWI76_RS10690 0.13 -1.2 2,259,037 + BWI76_RS10690 0.15 -0.3 2,259,456 - BWI76_RS10690 0.48 +1.4 2,259,729 - BWI76_RS10690 0.69 +1.0 2,259,731 - BWI76_RS10690 0.69 +0.9 2,259,828 + BWI76_RS10690 0.77 +0.2 2,259,909 - BWI76_RS10690 0.83 +1.2 2,260,534 - BWI76_RS10695 0.27 +1.2 2,260,554 - BWI76_RS10695 0.28 -1.5 2,260,643 - BWI76_RS10695 0.34 -3.3 2,260,715 + BWI76_RS10695 0.39 +0.0 2,260,715 + BWI76_RS10695 0.39 +0.3 2,260,771 + BWI76_RS10695 0.43 +0.5 2,260,845 - BWI76_RS10695 0.48 +0.9 2,261,044 + BWI76_RS10695 0.61 -1.8
Or see this region's nucleotide sequence