Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09715 and BWI76_RS09720 are separated by 17 nucleotides BWI76_RS09720 and BWI76_RS09725 are separated by 122 nucleotides BWI76_RS09725 and BWI76_RS09730 are separated by 53 nucleotides BWI76_RS09730 and BWI76_RS09735 are separated by 203 nucleotides
BWI76_RS09715: BWI76_RS09715 - hypothetical protein, at 2,036,291 to 2,037,556
_RS09715
BWI76_RS09720: BWI76_RS09720 - PTS ascorbate transporter subunit IIB, at 2,037,574 to 2,037,849
_RS09720
BWI76_RS09725: BWI76_RS09725 - MurR/RpiR family transcriptional regulator, at 2,037,972 to 2,038,829
_RS09725
BWI76_RS09730: BWI76_RS09730 - pyruvate formate lyase 1-activating protein, at 2,038,883 to 2,039,623
_RS09730
BWI76_RS09735: BWI76_RS09735 - formate acetyltransferase, at 2,039,827 to 2,042,109
_RS09735
Position (kb)
2037
2038
2039 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2036.998 kb on - strand, within BWI76_RS09715 at 2037.174 kb on + strand, within BWI76_RS09715 at 2037.228 kb on - strand, within BWI76_RS09715 at 2037.605 kb on + strand, within BWI76_RS09720 at 2037.623 kb on - strand, within BWI76_RS09720 at 2037.628 kb on - strand, within BWI76_RS09720 at 2037.728 kb on + strand, within BWI76_RS09720 at 2037.855 kb on + strand at 2038.181 kb on + strand, within BWI76_RS09725 at 2038.544 kb on + strand, within BWI76_RS09725 at 2038.585 kb on + strand, within BWI76_RS09725 at 2038.785 kb on + strand at 2038.881 kb on + strand at 2039.113 kb on - strand, within BWI76_RS09730 at 2039.385 kb on - strand, within BWI76_RS09730 at 2039.572 kb on - strand at 2039.746 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 2,036,998 - BWI76_RS09715 0.56 -1.3 2,037,174 + BWI76_RS09715 0.70 -1.1 2,037,228 - BWI76_RS09715 0.74 -0.3 2,037,605 + BWI76_RS09720 0.11 -0.4 2,037,623 - BWI76_RS09720 0.18 -1.4 2,037,628 - BWI76_RS09720 0.20 +0.1 2,037,728 + BWI76_RS09720 0.56 -1.4 2,037,855 + -1.0 2,038,181 + BWI76_RS09725 0.24 -1.6 2,038,544 + BWI76_RS09725 0.67 -1.6 2,038,585 + BWI76_RS09725 0.71 -2.9 2,038,785 + +0.5 2,038,881 + -0.7 2,039,113 - BWI76_RS09730 0.31 +1.2 2,039,385 - BWI76_RS09730 0.68 +0.5 2,039,572 - +0.2 2,039,746 - -0.6
Or see this region's nucleotide sequence