Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09470

Experiment: KLEB1 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09465 and BWI76_RS09470 are separated by 10 nucleotidesBWI76_RS09470 and BWI76_RS09475 overlap by 4 nucleotidesBWI76_RS09475 and BWI76_RS09480 are separated by 57 nucleotides BWI76_RS09465: BWI76_RS09465 - polyamine ABC transporter ATP-binding protein, at 1,981,746 to 1,982,924 _RS09465 BWI76_RS09470: BWI76_RS09470 - putrescine ABC transporter permease, at 1,982,935 to 1,983,888 _RS09470 BWI76_RS09475: BWI76_RS09475 - putrescine ABC transporter permease PotI, at 1,983,885 to 1,984,730 _RS09475 BWI76_RS09480: BWI76_RS09480 - hypothetical protein, at 1,984,788 to 1,985,276 _RS09480 Position (kb) 1982 1983 1984Strain fitness (log2 ratio) -3 -2 -1 0 1at 1982.027 kb on - strand, within BWI76_RS09465at 1982.245 kb on - strand, within BWI76_RS09465at 1982.359 kb on - strand, within BWI76_RS09465at 1982.535 kb on - strand, within BWI76_RS09465at 1982.595 kb on + strand, within BWI76_RS09465at 1982.603 kb on - strand, within BWI76_RS09465at 1982.603 kb on - strand, within BWI76_RS09465at 1982.613 kb on - strand, within BWI76_RS09465at 1982.724 kb on + strandat 1982.869 kb on - strandat 1983.548 kb on - strand, within BWI76_RS09470at 1983.640 kb on - strand, within BWI76_RS09470at 1983.739 kb on + strand, within BWI76_RS09470at 1983.802 kb on - strandat 1984.331 kb on + strand, within BWI76_RS09475at 1984.339 kb on - strand, within BWI76_RS09475at 1984.378 kb on + strand, within BWI76_RS09475at 1984.495 kb on + strand, within BWI76_RS09475at 1984.503 kb on - strand, within BWI76_RS09475at 1984.710 kb on - strandat 1984.777 kb on - strandat 1984.847 kb on - strand, within BWI76_RS09480at 1984.859 kb on + strand, within BWI76_RS09480

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1
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1,982,027 - BWI76_RS09465 0.24 +0.8
1,982,245 - BWI76_RS09465 0.42 +0.6
1,982,359 - BWI76_RS09465 0.52 +0.1
1,982,535 - BWI76_RS09465 0.67 -0.3
1,982,595 + BWI76_RS09465 0.72 +0.9
1,982,603 - BWI76_RS09465 0.73 -2.4
1,982,603 - BWI76_RS09465 0.73 +0.2
1,982,613 - BWI76_RS09465 0.74 -0.2
1,982,724 + +0.4
1,982,869 - -1.1
1,983,548 - BWI76_RS09470 0.64 -0.5
1,983,640 - BWI76_RS09470 0.74 -1.3
1,983,739 + BWI76_RS09470 0.84 -1.2
1,983,802 - +1.7
1,984,331 + BWI76_RS09475 0.53 -2.8
1,984,339 - BWI76_RS09475 0.54 +1.1
1,984,378 + BWI76_RS09475 0.58 +1.5
1,984,495 + BWI76_RS09475 0.72 -0.3
1,984,503 - BWI76_RS09475 0.73 -3.5
1,984,710 - -0.3
1,984,777 - -1.7
1,984,847 - BWI76_RS09480 0.12 -0.3
1,984,859 + BWI76_RS09480 0.15 -0.0

Or see this region's nucleotide sequence