Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09440 and BWI76_RS09445 overlap by 17 nucleotides BWI76_RS09445 and BWI76_RS09450 are separated by 103 nucleotides BWI76_RS09450 and BWI76_RS09455 are separated by 89 nucleotides BWI76_RS09455 and BWI76_RS09460 are separated by 349 nucleotides
BWI76_RS09440: BWI76_RS09440 - hypothetical protein, at 1,977,655 to 1,977,945
_RS09440
BWI76_RS09445: BWI76_RS09445 - NADPH-dependent oxidoreductase, at 1,977,929 to 1,978,651
_RS09445
BWI76_RS09450: BWI76_RS09450 - 30S ribosomal protein S6--L-glutamate ligase, at 1,978,755 to 1,979,657
_RS09450
BWI76_RS09455: BWI76_RS09455 - YbjN domain-containing protein, at 1,979,747 to 1,980,226
_RS09455
BWI76_RS09460: BWI76_RS09460 - spermidine/putrescine ABC transporter substrate-binding protein PotF, at 1,980,576 to 1,981,688
_RS09460
Position (kb)
1978
1979
1980 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1977.893 kb on - strand, within BWI76_RS09440 at 1978.172 kb on + strand, within BWI76_RS09445 at 1978.232 kb on + strand, within BWI76_RS09445 at 1978.300 kb on + strand, within BWI76_RS09445 at 1978.633 kb on + strand at 1978.728 kb on + strand at 1978.787 kb on + strand at 1979.000 kb on + strand, within BWI76_RS09450 at 1979.534 kb on + strand, within BWI76_RS09450 at 1979.932 kb on + strand, within BWI76_RS09455 at 1980.089 kb on - strand, within BWI76_RS09455 at 1980.110 kb on - strand, within BWI76_RS09455 at 1980.153 kb on - strand, within BWI76_RS09455 at 1980.174 kb on - strand, within BWI76_RS09455 at 1980.403 kb on - strand at 1980.419 kb on + strand at 1980.606 kb on + strand at 1980.611 kb on + strand at 1980.611 kb on + strand at 1980.619 kb on - strand at 1980.619 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 1,977,893 - BWI76_RS09440 0.82 -2.1 1,978,172 + BWI76_RS09445 0.34 -0.8 1,978,232 + BWI76_RS09445 0.42 -0.2 1,978,300 + BWI76_RS09445 0.51 -2.0 1,978,633 + -0.1 1,978,728 + +0.3 1,978,787 + -1.0 1,979,000 + BWI76_RS09450 0.27 -2.9 1,979,534 + BWI76_RS09450 0.86 -0.8 1,979,932 + BWI76_RS09455 0.39 +0.0 1,980,089 - BWI76_RS09455 0.71 -0.8 1,980,110 - BWI76_RS09455 0.76 +1.2 1,980,153 - BWI76_RS09455 0.85 -1.4 1,980,174 - BWI76_RS09455 0.89 -3.3 1,980,403 - +0.7 1,980,419 + -0.6 1,980,606 + +0.1 1,980,611 + -1.2 1,980,611 + +0.1 1,980,619 - -0.1 1,980,619 - +0.1
Or see this region's nucleotide sequence