Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09450

Experiment: KLEB1 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09440 and BWI76_RS09445 overlap by 17 nucleotidesBWI76_RS09445 and BWI76_RS09450 are separated by 103 nucleotidesBWI76_RS09450 and BWI76_RS09455 are separated by 89 nucleotidesBWI76_RS09455 and BWI76_RS09460 are separated by 349 nucleotides BWI76_RS09440: BWI76_RS09440 - hypothetical protein, at 1,977,655 to 1,977,945 _RS09440 BWI76_RS09445: BWI76_RS09445 - NADPH-dependent oxidoreductase, at 1,977,929 to 1,978,651 _RS09445 BWI76_RS09450: BWI76_RS09450 - 30S ribosomal protein S6--L-glutamate ligase, at 1,978,755 to 1,979,657 _RS09450 BWI76_RS09455: BWI76_RS09455 - YbjN domain-containing protein, at 1,979,747 to 1,980,226 _RS09455 BWI76_RS09460: BWI76_RS09460 - spermidine/putrescine ABC transporter substrate-binding protein PotF, at 1,980,576 to 1,981,688 _RS09460 Position (kb) 1978 1979 1980Strain fitness (log2 ratio) -3 -2 -1 0 1at 1977.893 kb on - strand, within BWI76_RS09440at 1978.172 kb on + strand, within BWI76_RS09445at 1978.232 kb on + strand, within BWI76_RS09445at 1978.300 kb on + strand, within BWI76_RS09445at 1978.633 kb on + strandat 1978.728 kb on + strandat 1978.787 kb on + strandat 1979.000 kb on + strand, within BWI76_RS09450at 1979.534 kb on + strand, within BWI76_RS09450at 1979.932 kb on + strand, within BWI76_RS09455at 1980.089 kb on - strand, within BWI76_RS09455at 1980.110 kb on - strand, within BWI76_RS09455at 1980.153 kb on - strand, within BWI76_RS09455at 1980.174 kb on - strand, within BWI76_RS09455at 1980.403 kb on - strandat 1980.419 kb on + strandat 1980.606 kb on + strandat 1980.611 kb on + strandat 1980.611 kb on + strandat 1980.619 kb on - strandat 1980.619 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1
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1,977,893 - BWI76_RS09440 0.82 -2.1
1,978,172 + BWI76_RS09445 0.34 -0.8
1,978,232 + BWI76_RS09445 0.42 -0.2
1,978,300 + BWI76_RS09445 0.51 -2.0
1,978,633 + -0.1
1,978,728 + +0.3
1,978,787 + -1.0
1,979,000 + BWI76_RS09450 0.27 -2.9
1,979,534 + BWI76_RS09450 0.86 -0.8
1,979,932 + BWI76_RS09455 0.39 +0.0
1,980,089 - BWI76_RS09455 0.71 -0.8
1,980,110 - BWI76_RS09455 0.76 +1.2
1,980,153 - BWI76_RS09455 0.85 -1.4
1,980,174 - BWI76_RS09455 0.89 -3.3
1,980,403 - +0.7
1,980,419 + -0.6
1,980,606 + +0.1
1,980,611 + -1.2
1,980,611 + +0.1
1,980,619 - -0.1
1,980,619 - +0.1

Or see this region's nucleotide sequence