Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07745 and BWI76_RS07750 are separated by 9 nucleotides BWI76_RS07750 and BWI76_RS07755 are separated by 13 nucleotides BWI76_RS07755 and BWI76_RS07760 overlap by 1 nucleotides
BWI76_RS07745: BWI76_RS07745 - isochorismate synthase EntC, at 1,643,316 to 1,644,491
_RS07745
BWI76_RS07750: BWI76_RS07750 - 2,3-dihydroxybenzoate-AMP ligase, at 1,644,501 to 1,646,108
_RS07750
BWI76_RS07755: BWI76_RS07755 - isochorismatase, at 1,646,122 to 1,646,973
_RS07755
BWI76_RS07760: BWI76_RS07760 - 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase, at 1,646,973 to 1,647,728
_RS07760
Position (kb)
1644
1645
1646
1647 Strain fitness (log2 ratio)
-1
0
1
2 at 1643.579 kb on + strand, within BWI76_RS07745 at 1643.587 kb on - strand, within BWI76_RS07745 at 1643.596 kb on + strand, within BWI76_RS07745 at 1643.670 kb on - strand, within BWI76_RS07745 at 1643.848 kb on + strand, within BWI76_RS07745 at 1643.848 kb on + strand, within BWI76_RS07745 at 1644.263 kb on - strand, within BWI76_RS07745 at 1644.795 kb on - strand, within BWI76_RS07750 at 1644.809 kb on + strand, within BWI76_RS07750 at 1644.844 kb on - strand, within BWI76_RS07750 at 1645.289 kb on - strand, within BWI76_RS07750 at 1645.853 kb on + strand, within BWI76_RS07750 at 1645.886 kb on - strand, within BWI76_RS07750 at 1645.886 kb on - strand, within BWI76_RS07750 at 1645.888 kb on + strand, within BWI76_RS07750 at 1645.929 kb on - strand, within BWI76_RS07750 at 1646.004 kb on + strand at 1646.103 kb on + strand at 1646.110 kb on + strand at 1646.283 kb on - strand, within BWI76_RS07755 at 1646.308 kb on - strand, within BWI76_RS07755 at 1646.351 kb on - strand, within BWI76_RS07755 at 1646.351 kb on - strand, within BWI76_RS07755 at 1647.053 kb on - strand, within BWI76_RS07760 at 1647.090 kb on - strand, within BWI76_RS07760
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 1,643,579 + BWI76_RS07745 0.22 +0.3 1,643,587 - BWI76_RS07745 0.23 -1.2 1,643,596 + BWI76_RS07745 0.24 -1.6 1,643,670 - BWI76_RS07745 0.30 -0.6 1,643,848 + BWI76_RS07745 0.45 +0.3 1,643,848 + BWI76_RS07745 0.45 -0.6 1,644,263 - BWI76_RS07745 0.81 +0.6 1,644,795 - BWI76_RS07750 0.18 -0.1 1,644,809 + BWI76_RS07750 0.19 +0.1 1,644,844 - BWI76_RS07750 0.21 +2.4 1,645,289 - BWI76_RS07750 0.49 +1.4 1,645,853 + BWI76_RS07750 0.84 +0.3 1,645,886 - BWI76_RS07750 0.86 +1.5 1,645,886 - BWI76_RS07750 0.86 +0.1 1,645,888 + BWI76_RS07750 0.86 +0.0 1,645,929 - BWI76_RS07750 0.89 +1.3 1,646,004 + +0.5 1,646,103 + +0.7 1,646,110 + +0.0 1,646,283 - BWI76_RS07755 0.19 -0.1 1,646,308 - BWI76_RS07755 0.22 +0.7 1,646,351 - BWI76_RS07755 0.27 +0.7 1,646,351 - BWI76_RS07755 0.27 -0.4 1,647,053 - BWI76_RS07760 0.11 +0.3 1,647,090 - BWI76_RS07760 0.15 +1.9
Or see this region's nucleotide sequence