Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06335 and BWI76_RS06340 are separated by 295 nucleotides BWI76_RS06340 and BWI76_RS06345 are separated by 147 nucleotides BWI76_RS06345 and BWI76_RS06350 are separated by 4 nucleotides
BWI76_RS06335: BWI76_RS06335 - nucleoside-specific channel-forming protein Tsx, at 1,333,308 to 1,334,192
_RS06335
BWI76_RS06340: BWI76_RS06340 - hypothetical protein, at 1,334,488 to 1,335,030
_RS06340
BWI76_RS06345: BWI76_RS06345 - transcriptional regulator NrdR, at 1,335,178 to 1,335,627
_RS06345
BWI76_RS06350: BWI76_RS06350 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase, at 1,335,632 to 1,336,735
_RS06350
Position (kb)
1334
1335
1336 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1333.544 kb on + strand, within BWI76_RS06335 at 1333.544 kb on + strand, within BWI76_RS06335 at 1333.751 kb on - strand, within BWI76_RS06335 at 1334.154 kb on + strand at 1334.154 kb on + strand at 1334.154 kb on + strand at 1334.154 kb on + strand at 1334.154 kb on + strand at 1334.205 kb on + strand at 1334.213 kb on - strand at 1334.217 kb on + strand at 1334.267 kb on - strand at 1334.457 kb on - strand at 1334.573 kb on - strand, within BWI76_RS06340 at 1335.621 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 1,333,544 + BWI76_RS06335 0.27 -3.1 1,333,544 + BWI76_RS06335 0.27 +0.5 1,333,751 - BWI76_RS06335 0.50 +0.3 1,334,154 + -3.4 1,334,154 + -1.9 1,334,154 + -0.5 1,334,154 + -0.2 1,334,154 + -3.1 1,334,205 + +0.3 1,334,213 - +0.3 1,334,217 + -0.9 1,334,267 - +0.2 1,334,457 - +0.1 1,334,573 - BWI76_RS06340 0.16 -1.7 1,335,621 + +1.3
Or see this region's nucleotide sequence