Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05520

Experiment: KLEB1 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05515 and BWI76_RS05520 are separated by 2 nucleotidesBWI76_RS05520 and BWI76_RS05525 are separated by 32 nucleotides BWI76_RS05515: BWI76_RS05515 - NADH:ubiquinone reductase (Na(+)-transporting) subunit F, at 1,156,452 to 1,157,675 _RS05515 BWI76_RS05520: BWI76_RS05520 - hypothetical protein, at 1,157,678 to 1,157,893 _RS05520 BWI76_RS05525: BWI76_RS05525 - glycerophosphodiester phosphodiesterase, at 1,157,926 to 1,158,783 _RS05525 Position (kb) 1157 1158Strain fitness (log2 ratio) -2 -1 0 1 2at 1156.733 kb on + strand, within BWI76_RS05515at 1156.858 kb on - strand, within BWI76_RS05515at 1157.126 kb on - strand, within BWI76_RS05515at 1157.340 kb on + strand, within BWI76_RS05515at 1157.773 kb on - strand, within BWI76_RS05520at 1157.809 kb on + strand, within BWI76_RS05520at 1157.820 kb on + strand, within BWI76_RS05520at 1157.820 kb on + strand, within BWI76_RS05520at 1157.828 kb on - strand, within BWI76_RS05520at 1158.058 kb on + strand, within BWI76_RS05525at 1158.058 kb on + strand, within BWI76_RS05525at 1158.329 kb on - strand, within BWI76_RS05525at 1158.330 kb on - strand, within BWI76_RS05525at 1158.542 kb on - strand, within BWI76_RS05525

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1
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1,156,733 + BWI76_RS05515 0.23 +0.1
1,156,858 - BWI76_RS05515 0.33 +1.3
1,157,126 - BWI76_RS05515 0.55 +1.1
1,157,340 + BWI76_RS05515 0.73 +0.3
1,157,773 - BWI76_RS05520 0.44 -1.5
1,157,809 + BWI76_RS05520 0.61 -1.6
1,157,820 + BWI76_RS05520 0.66 -0.2
1,157,820 + BWI76_RS05520 0.66 +1.9
1,157,828 - BWI76_RS05520 0.69 -1.2
1,158,058 + BWI76_RS05525 0.15 +0.8
1,158,058 + BWI76_RS05525 0.15 +2.0
1,158,329 - BWI76_RS05525 0.47 -2.7
1,158,330 - BWI76_RS05525 0.47 -0.6
1,158,542 - BWI76_RS05525 0.72 +0.4

Or see this region's nucleotide sequence