Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02940 and BWI76_RS02945 are separated by 260 nucleotides BWI76_RS02945 and BWI76_RS02950 are separated by 79 nucleotides
BWI76_RS02940: BWI76_RS02940 - RpiR family transcriptional regulator, at 601,371 to 602,114
_RS02940
BWI76_RS02945: BWI76_RS02945 - PTS sugar transporter subunit IIB, at 602,375 to 602,683
_RS02945
BWI76_RS02950: BWI76_RS02950 - 6-phospho-beta-glucosidase, at 602,763 to 604,070
_RS02950
Position (kb) 602
603 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 601.395 kb on - strand at 601.442 kb on - strand at 601.446 kb on - strand, within BWI76_RS02940 at 601.535 kb on + strand, within BWI76_RS02940 at 601.699 kb on + strand, within BWI76_RS02940 at 601.887 kb on - strand, within BWI76_RS02940 at 602.079 kb on + strand at 602.344 kb on - strand at 602.382 kb on + strand at 602.390 kb on - strand at 602.575 kb on + strand at 602.669 kb on + strand at 602.731 kb on + strand at 602.811 kb on - strand at 602.811 kb on - strand at 602.827 kb on + strand at 602.827 kb on + strand at 602.835 kb on - strand at 602.927 kb on + strand, within BWI76_RS02950 at 603.340 kb on + strand, within BWI76_RS02950 at 603.402 kb on + strand, within BWI76_RS02950 at 603.673 kb on + strand, within BWI76_RS02950
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 601,395 - -0.4 601,442 - -0.3 601,446 - BWI76_RS02940 0.10 -2.3 601,535 + BWI76_RS02940 0.22 +0.3 601,699 + BWI76_RS02940 0.44 -2.2 601,887 - BWI76_RS02940 0.69 -0.9 602,079 + -3.7 602,344 - -2.5 602,382 + -0.0 602,390 - -1.0 602,575 + -1.6 602,669 + +0.6 602,731 + +0.7 602,811 - -1.9 602,811 - +0.0 602,827 + -0.3 602,827 + -0.4 602,835 - +0.3 602,927 + BWI76_RS02950 0.13 -0.5 603,340 + BWI76_RS02950 0.44 -0.3 603,402 + BWI76_RS02950 0.49 +0.0 603,673 + BWI76_RS02950 0.70 -0.2
Or see this region's nucleotide sequence