Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02085 and BWI76_RS02090 overlap by 4 nucleotides BWI76_RS02090 and BWI76_RS02095 are separated by 182 nucleotides BWI76_RS02095 and BWI76_RS02100 are separated by 92 nucleotides BWI76_RS02100 and BWI76_RS02105 are separated by 67 nucleotides
BWI76_RS02085: BWI76_RS02085 - cation acetate symporter, at 429,691 to 431,340
_RS02085
BWI76_RS02090: BWI76_RS02090 - membrane protein, at 431,337 to 431,651
_RS02090
BWI76_RS02095: BWI76_RS02095 - acetyl-coenzyme A synthetase, at 431,834 to 433,792
_RS02095
BWI76_RS02100: BWI76_RS02100 - hypothetical protein, at 433,885 to 434,136
_RS02100
BWI76_RS02105: BWI76_RS02105 - glutamate/aspartate:proton symporter GltP, at 434,204 to 435,517
_RS02105
Position (kb) 431
432
433
434 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 430.888 kb on + strand, within BWI76_RS02085 at 430.914 kb on + strand, within BWI76_RS02085 at 430.922 kb on - strand, within BWI76_RS02085 at 430.976 kb on - strand, within BWI76_RS02085 at 431.056 kb on - strand, within BWI76_RS02085 at 431.056 kb on - strand, within BWI76_RS02085 at 431.082 kb on - strand, within BWI76_RS02085 at 431.192 kb on + strand at 431.200 kb on - strand at 431.265 kb on - strand at 431.265 kb on - strand at 431.270 kb on - strand at 431.323 kb on + strand at 431.344 kb on + strand at 431.502 kb on - strand, within BWI76_RS02090 at 431.536 kb on - strand, within BWI76_RS02090 at 431.559 kb on - strand, within BWI76_RS02090 at 431.668 kb on - strand at 431.726 kb on - strand at 431.728 kb on + strand at 431.762 kb on + strand at 431.770 kb on - strand at 431.815 kb on + strand at 431.847 kb on + strand at 431.901 kb on + strand at 432.063 kb on + strand, within BWI76_RS02095 at 432.066 kb on - strand at 432.071 kb on - strand, within BWI76_RS02095 at 432.094 kb on + strand, within BWI76_RS02095 at 432.094 kb on + strand, within BWI76_RS02095 at 432.110 kb on + strand, within BWI76_RS02095 at 432.126 kb on + strand, within BWI76_RS02095 at 432.297 kb on + strand, within BWI76_RS02095 at 432.381 kb on + strand, within BWI76_RS02095 at 432.457 kb on + strand, within BWI76_RS02095 at 432.526 kb on + strand, within BWI76_RS02095 at 432.620 kb on - strand, within BWI76_RS02095 at 432.715 kb on + strand, within BWI76_RS02095 at 432.788 kb on + strand, within BWI76_RS02095 at 432.975 kb on + strand, within BWI76_RS02095 at 432.983 kb on - strand, within BWI76_RS02095 at 433.039 kb on + strand, within BWI76_RS02095 at 433.080 kb on + strand, within BWI76_RS02095 at 433.138 kb on - strand, within BWI76_RS02095 at 433.182 kb on + strand, within BWI76_RS02095 at 433.413 kb on + strand, within BWI76_RS02095 at 433.513 kb on - strand, within BWI76_RS02095 at 433.716 kb on + strand at 433.726 kb on + strand at 434.147 kb on + strand at 434.196 kb on + strand at 434.258 kb on - strand at 434.275 kb on - strand at 434.316 kb on + strand at 434.353 kb on - strand, within BWI76_RS02105 at 434.387 kb on + strand, within BWI76_RS02105 at 434.481 kb on + strand, within BWI76_RS02105 at 434.484 kb on - strand, within BWI76_RS02105 at 434.502 kb on + strand, within BWI76_RS02105 at 434.510 kb on - strand, within BWI76_RS02105 at 434.600 kb on + strand, within BWI76_RS02105 at 434.608 kb on - strand, within BWI76_RS02105
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 430,888 + BWI76_RS02085 0.73 -0.2 430,914 + BWI76_RS02085 0.74 -0.0 430,922 - BWI76_RS02085 0.75 -0.9 430,976 - BWI76_RS02085 0.78 +0.7 431,056 - BWI76_RS02085 0.83 -0.0 431,056 - BWI76_RS02085 0.83 -0.3 431,082 - BWI76_RS02085 0.84 -0.4 431,192 + -1.5 431,200 - -0.4 431,265 - -0.3 431,265 - -0.2 431,270 - -1.1 431,323 + -0.6 431,344 + -2.0 431,502 - BWI76_RS02090 0.52 -1.0 431,536 - BWI76_RS02090 0.63 -2.0 431,559 - BWI76_RS02090 0.70 -1.7 431,668 - +0.2 431,726 - +1.6 431,728 + -1.7 431,762 + -0.5 431,770 - -0.4 431,815 + -0.2 431,847 + +0.1 431,901 + +0.4 432,063 + BWI76_RS02095 0.12 -1.9 432,066 - +1.0 432,071 - BWI76_RS02095 0.12 -0.4 432,094 + BWI76_RS02095 0.13 -0.5 432,094 + BWI76_RS02095 0.13 -0.4 432,110 + BWI76_RS02095 0.14 +0.7 432,126 + BWI76_RS02095 0.15 +0.1 432,297 + BWI76_RS02095 0.24 -0.4 432,381 + BWI76_RS02095 0.28 -0.4 432,457 + BWI76_RS02095 0.32 -0.3 432,526 + BWI76_RS02095 0.35 -2.8 432,620 - BWI76_RS02095 0.40 -0.3 432,715 + BWI76_RS02095 0.45 -0.6 432,788 + BWI76_RS02095 0.49 -1.2 432,975 + BWI76_RS02095 0.58 -3.3 432,983 - BWI76_RS02095 0.59 -0.6 433,039 + BWI76_RS02095 0.62 +0.8 433,080 + BWI76_RS02095 0.64 -0.8 433,138 - BWI76_RS02095 0.67 +0.3 433,182 + BWI76_RS02095 0.69 -0.4 433,413 + BWI76_RS02095 0.81 +0.9 433,513 - BWI76_RS02095 0.86 -4.2 433,716 + +0.3 433,726 + +0.1 434,147 + -1.6 434,196 + -1.9 434,258 - -0.0 434,275 - +1.7 434,316 + +0.1 434,353 - BWI76_RS02105 0.11 +0.1 434,387 + BWI76_RS02105 0.14 +0.7 434,481 + BWI76_RS02105 0.21 -1.4 434,484 - BWI76_RS02105 0.21 -2.1 434,502 + BWI76_RS02105 0.23 +0.3 434,510 - BWI76_RS02105 0.23 -0.4 434,600 + BWI76_RS02105 0.30 -2.0 434,608 - BWI76_RS02105 0.31 -2.4
Or see this region's nucleotide sequence