Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01835

Experiment: KLEB1 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01830 and BWI76_RS01835 are separated by 27 nucleotidesBWI76_RS01835 and BWI76_RS01840 are separated by 55 nucleotides BWI76_RS01830: BWI76_RS01830 - maltose ABC transporter, substrate-binding component MalE (from data), at 373,299 to 374,489 _RS01830 BWI76_RS01835: BWI76_RS01835 - chemotaxis protein CheW, at 374,517 to 374,804 _RS01835 BWI76_RS01840: BWI76_RS01840 - maltose ABC transporter, ATPase component (MalK) (from data), at 374,860 to 375,969 _RS01840 Position (kb) 374 375Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 373.643 kb on + strand, within BWI76_RS01830at 373.681 kb on + strand, within BWI76_RS01830at 373.684 kb on - strand, within BWI76_RS01830at 373.716 kb on - strand, within BWI76_RS01830at 373.860 kb on + strand, within BWI76_RS01830at 373.969 kb on - strand, within BWI76_RS01830at 373.978 kb on + strand, within BWI76_RS01830at 374.161 kb on + strand, within BWI76_RS01830at 374.580 kb on + strand, within BWI76_RS01835at 374.586 kb on + strand, within BWI76_RS01835at 374.594 kb on - strand, within BWI76_RS01835at 374.642 kb on + strand, within BWI76_RS01835at 374.789 kb on + strandat 375.168 kb on - strand, within BWI76_RS01840at 375.681 kb on + strand, within BWI76_RS01840

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1
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373,643 + BWI76_RS01830 0.29 -0.1
373,681 + BWI76_RS01830 0.32 -0.7
373,684 - BWI76_RS01830 0.32 -0.7
373,716 - BWI76_RS01830 0.35 -0.3
373,860 + BWI76_RS01830 0.47 +0.3
373,969 - BWI76_RS01830 0.56 +0.3
373,978 + BWI76_RS01830 0.57 +1.2
374,161 + BWI76_RS01830 0.72 +0.1
374,580 + BWI76_RS01835 0.22 -4.8
374,586 + BWI76_RS01835 0.24 -0.8
374,594 - BWI76_RS01835 0.27 -0.9
374,642 + BWI76_RS01835 0.43 -1.5
374,789 + -0.1
375,168 - BWI76_RS01840 0.28 -0.4
375,681 + BWI76_RS01840 0.74 -0.6

Or see this region's nucleotide sequence