Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22275

Experiment: KLEB1 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22270 and BWI76_RS22275 are separated by 11 nucleotidesBWI76_RS22275 and BWI76_RS22280 are separated by 12 nucleotidesBWI76_RS22280 and BWI76_RS22285 are separated by 134 nucleotides BWI76_RS22270: BWI76_RS22270 - nickel transporter, at 4,641,652 to 4,642,665 _RS22270 BWI76_RS22275: BWI76_RS22275 - carbamoyltransferase HypF, at 4,642,677 to 4,644,896 _RS22275 BWI76_RS22280: BWI76_RS22280 - formate dehydrogenase-H ferredoxin subunit, at 4,644,909 to 4,645,436 _RS22280 BWI76_RS22285: BWI76_RS22285 - transcriptional regulator, at 4,645,571 to 4,646,584 _RS22285 Position (kb) 4642 4643 4644 4645Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 4641.721 kb on - strandat 4641.776 kb on - strand, within BWI76_RS22270at 4641.839 kb on + strand, within BWI76_RS22270at 4641.847 kb on - strand, within BWI76_RS22270at 4641.893 kb on + strand, within BWI76_RS22270at 4641.930 kb on - strand, within BWI76_RS22270at 4642.218 kb on + strand, within BWI76_RS22270at 4642.226 kb on - strand, within BWI76_RS22270at 4642.582 kb on + strandat 4642.590 kb on - strandat 4642.671 kb on - strandat 4642.674 kb on + strandat 4642.682 kb on - strandat 4642.944 kb on + strand, within BWI76_RS22275at 4642.973 kb on + strand, within BWI76_RS22275at 4643.190 kb on + strand, within BWI76_RS22275at 4643.292 kb on - strand, within BWI76_RS22275at 4643.427 kb on - strand, within BWI76_RS22275at 4643.592 kb on - strand, within BWI76_RS22275at 4643.607 kb on - strand, within BWI76_RS22275at 4644.032 kb on + strand, within BWI76_RS22275at 4644.147 kb on - strand, within BWI76_RS22275at 4644.471 kb on + strand, within BWI76_RS22275at 4644.491 kb on - strand, within BWI76_RS22275at 4644.506 kb on + strand, within BWI76_RS22275at 4644.569 kb on + strand, within BWI76_RS22275at 4644.889 kb on - strandat 4644.923 kb on - strandat 4644.923 kb on - strandat 4644.961 kb on - strandat 4644.963 kb on + strand, within BWI76_RS22280at 4645.001 kb on + strand, within BWI76_RS22280at 4645.083 kb on - strand, within BWI76_RS22280at 4645.202 kb on + strand, within BWI76_RS22280at 4645.225 kb on + strand, within BWI76_RS22280at 4645.379 kb on + strand, within BWI76_RS22280at 4645.409 kb on - strandat 4645.770 kb on + strand, within BWI76_RS22285at 4645.778 kb on - strand, within BWI76_RS22285at 4645.778 kb on - strand, within BWI76_RS22285at 4645.789 kb on + strand, within BWI76_RS22285

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1
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4,641,721 - +0.1
4,641,776 - BWI76_RS22270 0.12 +5.0
4,641,839 + BWI76_RS22270 0.18 -0.4
4,641,847 - BWI76_RS22270 0.19 +0.4
4,641,893 + BWI76_RS22270 0.24 -0.2
4,641,930 - BWI76_RS22270 0.27 +0.1
4,642,218 + BWI76_RS22270 0.56 +0.1
4,642,226 - BWI76_RS22270 0.57 -3.2
4,642,582 + +0.5
4,642,590 - -0.4
4,642,671 - -0.2
4,642,674 + +0.6
4,642,682 - -3.1
4,642,944 + BWI76_RS22275 0.12 -0.0
4,642,973 + BWI76_RS22275 0.13 +1.2
4,643,190 + BWI76_RS22275 0.23 +0.3
4,643,292 - BWI76_RS22275 0.28 +0.1
4,643,427 - BWI76_RS22275 0.34 +1.5
4,643,592 - BWI76_RS22275 0.41 +0.9
4,643,607 - BWI76_RS22275 0.42 +0.6
4,644,032 + BWI76_RS22275 0.61 +0.4
4,644,147 - BWI76_RS22275 0.66 -1.1
4,644,471 + BWI76_RS22275 0.81 +1.6
4,644,491 - BWI76_RS22275 0.82 -0.1
4,644,506 + BWI76_RS22275 0.82 +0.7
4,644,569 + BWI76_RS22275 0.85 +0.6
4,644,889 - -0.2
4,644,923 - +1.2
4,644,923 - +0.4
4,644,961 - -0.1
4,644,963 + BWI76_RS22280 0.10 +0.6
4,645,001 + BWI76_RS22280 0.17 -0.1
4,645,083 - BWI76_RS22280 0.33 -0.1
4,645,202 + BWI76_RS22280 0.55 -0.2
4,645,225 + BWI76_RS22280 0.60 -0.7
4,645,379 + BWI76_RS22280 0.89 +0.1
4,645,409 - +0.2
4,645,770 + BWI76_RS22285 0.20 +0.7
4,645,778 - BWI76_RS22285 0.20 -3.2
4,645,778 - BWI76_RS22285 0.20 +0.3
4,645,789 + BWI76_RS22285 0.21 -0.0

Or see this region's nucleotide sequence