Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08935 and BWI76_RS08940 are separated by 68 nucleotides BWI76_RS08940 and BWI76_RS08945 are separated by 45 nucleotides BWI76_RS08945 and BWI76_RS08950 are separated by 296 nucleotides
BWI76_RS08935: BWI76_RS08935 - hypothetical protein, at 1,882,300 to 1,882,566
_RS08935
BWI76_RS08940: BWI76_RS08940 - PKHD-type hydroxylase, at 1,882,635 to 1,883,312
_RS08940
BWI76_RS08945: BWI76_RS08945 - catecholate siderophore receptor Fiu, at 1,883,358 to 1,885,646
_RS08945
BWI76_RS08950: BWI76_RS08950 - Asp/Glu/hydantoin racemase, at 1,885,943 to 1,886,557
_RS08950
Position (kb)
1883
1884
1885
1886 Strain fitness (log2 ratio)
-2
-1
0
1 at 1882.571 kb on - strand at 1882.576 kb on + strand at 1882.589 kb on + strand at 1882.591 kb on + strand at 1882.661 kb on + strand at 1882.839 kb on + strand, within BWI76_RS08940 at 1882.898 kb on + strand, within BWI76_RS08940 at 1883.066 kb on - strand, within BWI76_RS08940 at 1883.139 kb on + strand, within BWI76_RS08940 at 1883.152 kb on + strand, within BWI76_RS08940 at 1883.177 kb on - strand, within BWI76_RS08940 at 1883.457 kb on - strand at 1883.539 kb on + strand at 1883.685 kb on + strand, within BWI76_RS08945 at 1883.730 kb on + strand, within BWI76_RS08945 at 1883.954 kb on + strand, within BWI76_RS08945 at 1884.583 kb on + strand, within BWI76_RS08945 at 1884.664 kb on + strand, within BWI76_RS08945 at 1885.862 kb on + strand at 1886.031 kb on - strand, within BWI76_RS08950 at 1886.102 kb on - strand, within BWI76_RS08950 at 1886.131 kb on - strand, within BWI76_RS08950 at 1886.365 kb on + strand, within BWI76_RS08950 at 1886.439 kb on + strand, within BWI76_RS08950
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 1,882,571 - -0.5 1,882,576 + +0.1 1,882,589 + -2.6 1,882,591 + +1.2 1,882,661 + -0.6 1,882,839 + BWI76_RS08940 0.30 +1.0 1,882,898 + BWI76_RS08940 0.39 -0.2 1,883,066 - BWI76_RS08940 0.64 -1.3 1,883,139 + BWI76_RS08940 0.74 +0.6 1,883,152 + BWI76_RS08940 0.76 -2.1 1,883,177 - BWI76_RS08940 0.80 -0.5 1,883,457 - +0.8 1,883,539 + +0.2 1,883,685 + BWI76_RS08945 0.14 -0.1 1,883,730 + BWI76_RS08945 0.16 -0.0 1,883,954 + BWI76_RS08945 0.26 +0.0 1,884,583 + BWI76_RS08945 0.54 +0.5 1,884,664 + BWI76_RS08945 0.57 +0.1 1,885,862 + -0.3 1,886,031 - BWI76_RS08950 0.14 -2.2 1,886,102 - BWI76_RS08950 0.26 -0.4 1,886,131 - BWI76_RS08950 0.31 +1.3 1,886,365 + BWI76_RS08950 0.69 -0.9 1,886,439 + BWI76_RS08950 0.81 +0.0
Or see this region's nucleotide sequence