Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26350 and BWI76_RS26355 are separated by 153 nucleotides BWI76_RS26355 and BWI76_RS26360 are separated by 31 nucleotides BWI76_RS26360 and BWI76_RS26365 are separated by 248 nucleotides
BWI76_RS26350: BWI76_RS26350 - branched chain amino acid ABC transporter substrate-binding protein, at 5,454,357 to 5,455,466
_RS26350
BWI76_RS26355: BWI76_RS26355 - hypothetical protein, at 5,455,620 to 5,455,855
_RS26355
BWI76_RS26360: BWI76_RS26360 - aspartate 1-decarboxylase autocleavage activator PanM, at 5,455,887 to 5,456,273
_RS26360
BWI76_RS26365: BWI76_RS26365 - branched chain amino acid ABC transporter substrate-binding protein, at 5,456,522 to 5,457,640
_RS26365
Position (kb) 5455
5456
5457 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5454.926 kb on - strand, within BWI76_RS26350 at 5455.090 kb on - strand, within BWI76_RS26350 at 5455.170 kb on - strand, within BWI76_RS26350 at 5455.229 kb on + strand, within BWI76_RS26350 at 5455.248 kb on + strand, within BWI76_RS26350 at 5455.349 kb on + strand, within BWI76_RS26350 at 5455.492 kb on - strand at 5455.552 kb on + strand at 5455.770 kb on + strand, within BWI76_RS26355 at 5455.770 kb on + strand, within BWI76_RS26355 at 5455.775 kb on + strand, within BWI76_RS26355 at 5455.778 kb on - strand, within BWI76_RS26355 at 5455.783 kb on - strand, within BWI76_RS26355 at 5455.924 kb on - strand at 5455.924 kb on - strand at 5456.600 kb on - strand at 5456.689 kb on - strand, within BWI76_RS26365 at 5456.710 kb on + strand, within BWI76_RS26365 at 5456.850 kb on + strand, within BWI76_RS26365 at 5456.858 kb on - strand, within BWI76_RS26365 at 5457.085 kb on - strand, within BWI76_RS26365 at 5457.092 kb on + strand, within BWI76_RS26365 at 5457.105 kb on + strand, within BWI76_RS26365 at 5457.262 kb on + strand, within BWI76_RS26365
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 5,454,926 - BWI76_RS26350 0.51 +0.5 5,455,090 - BWI76_RS26350 0.66 -1.1 5,455,170 - BWI76_RS26350 0.73 +0.4 5,455,229 + BWI76_RS26350 0.79 +1.9 5,455,248 + BWI76_RS26350 0.80 -2.0 5,455,349 + BWI76_RS26350 0.89 +0.8 5,455,492 - +2.4 5,455,552 + -0.5 5,455,770 + BWI76_RS26355 0.64 +0.4 5,455,770 + BWI76_RS26355 0.64 +1.1 5,455,775 + BWI76_RS26355 0.66 +0.3 5,455,778 - BWI76_RS26355 0.67 +0.3 5,455,783 - BWI76_RS26355 0.69 -0.5 5,455,924 - +0.3 5,455,924 - -0.3 5,456,600 - +0.2 5,456,689 - BWI76_RS26365 0.15 +0.2 5,456,710 + BWI76_RS26365 0.17 +0.4 5,456,850 + BWI76_RS26365 0.29 -0.7 5,456,858 - BWI76_RS26365 0.30 +0.6 5,457,085 - BWI76_RS26365 0.50 -0.4 5,457,092 + BWI76_RS26365 0.51 -0.5 5,457,105 + BWI76_RS26365 0.52 +0.9 5,457,262 + BWI76_RS26365 0.66 -0.9
Or see this region's nucleotide sequence