Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25405 and BWI76_RS25410 are separated by 210 nucleotides BWI76_RS25410 and BWI76_RS25415 overlap by 29 nucleotides BWI76_RS25415 and BWI76_RS25420 are separated by 131 nucleotides
BWI76_RS25405: BWI76_RS25405 - anion transporter, at 5,280,341 to 5,281,609
_RS25405
BWI76_RS25410: BWI76_RS25410 - GntR family transcriptional regulator with, at 5,281,820 to 5,282,485
_RS25410
BWI76_RS25415: BWI76_RS25415 - GntR family transcriptional regulator, at 5,282,457 to 5,283,101
_RS25415
BWI76_RS25420: BWI76_RS25420 - malate dehydrogenase, at 5,283,233 to 5,284,171
_RS25420
Position (kb) 5282
5283
5284 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5281.519 kb on + strand at 5281.908 kb on + strand, within BWI76_RS25410 at 5281.984 kb on + strand, within BWI76_RS25410 at 5281.984 kb on + strand, within BWI76_RS25410 at 5281.984 kb on + strand, within BWI76_RS25410 at 5282.076 kb on + strand, within BWI76_RS25410 at 5282.103 kb on - strand, within BWI76_RS25410 at 5282.141 kb on + strand, within BWI76_RS25410 at 5282.141 kb on - strand, within BWI76_RS25410 at 5282.209 kb on - strand, within BWI76_RS25410 at 5282.209 kb on - strand, within BWI76_RS25410 at 5282.212 kb on + strand, within BWI76_RS25410 at 5282.301 kb on - strand, within BWI76_RS25410 at 5282.347 kb on - strand, within BWI76_RS25410 at 5282.351 kb on - strand, within BWI76_RS25410 at 5282.365 kb on + strand at 5282.477 kb on - strand at 5282.493 kb on - strand at 5282.875 kb on + strand, within BWI76_RS25415 at 5283.028 kb on - strand, within BWI76_RS25415 at 5283.192 kb on - strand at 5283.383 kb on + strand, within BWI76_RS25420 at 5283.633 kb on + strand, within BWI76_RS25420 at 5283.826 kb on + strand, within BWI76_RS25420 at 5283.844 kb on - strand, within BWI76_RS25420 at 5283.856 kb on - strand, within BWI76_RS25420 at 5284.085 kb on + strand at 5284.092 kb on - strand at 5284.092 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 5,281,519 + -1.0 5,281,908 + BWI76_RS25410 0.13 +0.6 5,281,984 + BWI76_RS25410 0.25 +0.3 5,281,984 + BWI76_RS25410 0.25 -0.4 5,281,984 + BWI76_RS25410 0.25 -1.8 5,282,076 + BWI76_RS25410 0.38 -0.5 5,282,103 - BWI76_RS25410 0.42 +0.5 5,282,141 + BWI76_RS25410 0.48 +0.2 5,282,141 - BWI76_RS25410 0.48 +0.6 5,282,209 - BWI76_RS25410 0.58 -0.7 5,282,209 - BWI76_RS25410 0.58 -0.4 5,282,212 + BWI76_RS25410 0.59 +0.5 5,282,301 - BWI76_RS25410 0.72 +0.3 5,282,347 - BWI76_RS25410 0.79 -0.0 5,282,351 - BWI76_RS25410 0.80 -0.8 5,282,365 + -1.5 5,282,477 - -1.6 5,282,493 - -2.4 5,282,875 + BWI76_RS25415 0.65 -1.0 5,283,028 - BWI76_RS25415 0.89 -0.7 5,283,192 - -0.2 5,283,383 + BWI76_RS25420 0.16 +1.0 5,283,633 + BWI76_RS25420 0.43 +0.9 5,283,826 + BWI76_RS25420 0.63 -3.8 5,283,844 - BWI76_RS25420 0.65 +0.4 5,283,856 - BWI76_RS25420 0.66 -0.5 5,284,085 + -1.3 5,284,092 - -1.9 5,284,092 - -0.7
Or see this region's nucleotide sequence