Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22270 and BWI76_RS22275 are separated by 11 nucleotides BWI76_RS22275 and BWI76_RS22280 are separated by 12 nucleotides BWI76_RS22280 and BWI76_RS22285 are separated by 134 nucleotides
BWI76_RS22270: BWI76_RS22270 - nickel transporter, at 4,641,652 to 4,642,665
_RS22270
BWI76_RS22275: BWI76_RS22275 - carbamoyltransferase HypF, at 4,642,677 to 4,644,896
_RS22275
BWI76_RS22280: BWI76_RS22280 - formate dehydrogenase-H ferredoxin subunit, at 4,644,909 to 4,645,436
_RS22280
BWI76_RS22285: BWI76_RS22285 - transcriptional regulator, at 4,645,571 to 4,646,584
_RS22285
Position (kb)
4642
4643
4644
4645 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4641.721 kb on - strand at 4641.776 kb on - strand, within BWI76_RS22270 at 4641.839 kb on + strand, within BWI76_RS22270 at 4641.847 kb on - strand, within BWI76_RS22270 at 4641.893 kb on + strand, within BWI76_RS22270 at 4641.930 kb on - strand, within BWI76_RS22270 at 4642.218 kb on + strand, within BWI76_RS22270 at 4642.226 kb on - strand, within BWI76_RS22270 at 4642.582 kb on + strand at 4642.590 kb on - strand at 4642.671 kb on - strand at 4642.674 kb on + strand at 4642.682 kb on - strand at 4642.944 kb on + strand, within BWI76_RS22275 at 4642.973 kb on + strand, within BWI76_RS22275 at 4643.190 kb on + strand, within BWI76_RS22275 at 4643.292 kb on - strand, within BWI76_RS22275 at 4643.427 kb on - strand, within BWI76_RS22275 at 4643.592 kb on - strand, within BWI76_RS22275 at 4643.607 kb on - strand, within BWI76_RS22275 at 4644.032 kb on + strand, within BWI76_RS22275 at 4644.147 kb on - strand, within BWI76_RS22275 at 4644.471 kb on + strand, within BWI76_RS22275 at 4644.491 kb on - strand, within BWI76_RS22275 at 4644.506 kb on + strand, within BWI76_RS22275 at 4644.569 kb on + strand, within BWI76_RS22275 at 4644.889 kb on - strand at 4644.923 kb on - strand at 4644.923 kb on - strand at 4644.961 kb on - strand at 4644.963 kb on + strand, within BWI76_RS22280 at 4645.001 kb on + strand, within BWI76_RS22280 at 4645.083 kb on - strand, within BWI76_RS22280 at 4645.202 kb on + strand, within BWI76_RS22280 at 4645.225 kb on + strand, within BWI76_RS22280 at 4645.379 kb on + strand, within BWI76_RS22280 at 4645.409 kb on - strand at 4645.770 kb on + strand, within BWI76_RS22285 at 4645.778 kb on - strand, within BWI76_RS22285 at 4645.778 kb on - strand, within BWI76_RS22285 at 4645.789 kb on + strand, within BWI76_RS22285
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 4,641,721 - +0.1 4,641,776 - BWI76_RS22270 0.12 +2.7 4,641,839 + BWI76_RS22270 0.18 +0.3 4,641,847 - BWI76_RS22270 0.19 +0.3 4,641,893 + BWI76_RS22270 0.24 -0.4 4,641,930 - BWI76_RS22270 0.27 +0.8 4,642,218 + BWI76_RS22270 0.56 -2.2 4,642,226 - BWI76_RS22270 0.57 +0.4 4,642,582 + -0.9 4,642,590 - -0.7 4,642,671 - -0.2 4,642,674 + +0.1 4,642,682 - -0.5 4,642,944 + BWI76_RS22275 0.12 +0.5 4,642,973 + BWI76_RS22275 0.13 +1.7 4,643,190 + BWI76_RS22275 0.23 +0.7 4,643,292 - BWI76_RS22275 0.28 +0.7 4,643,427 - BWI76_RS22275 0.34 +1.7 4,643,592 - BWI76_RS22275 0.41 +1.0 4,643,607 - BWI76_RS22275 0.42 -2.3 4,644,032 + BWI76_RS22275 0.61 +0.2 4,644,147 - BWI76_RS22275 0.66 -0.6 4,644,471 + BWI76_RS22275 0.81 +3.0 4,644,491 - BWI76_RS22275 0.82 +0.7 4,644,506 + BWI76_RS22275 0.82 +1.9 4,644,569 + BWI76_RS22275 0.85 +0.2 4,644,889 - +1.9 4,644,923 - +1.0 4,644,923 - +0.3 4,644,961 - -2.7 4,644,963 + BWI76_RS22280 0.10 +0.1 4,645,001 + BWI76_RS22280 0.17 +0.1 4,645,083 - BWI76_RS22280 0.33 +0.8 4,645,202 + BWI76_RS22280 0.55 +1.5 4,645,225 + BWI76_RS22280 0.60 -0.7 4,645,379 + BWI76_RS22280 0.89 +0.2 4,645,409 - -0.1 4,645,770 + BWI76_RS22285 0.20 +0.5 4,645,778 - BWI76_RS22285 0.20 +0.3 4,645,778 - BWI76_RS22285 0.20 +0.8 4,645,789 + BWI76_RS22285 0.21 -0.3
Or see this region's nucleotide sequence