Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21640 and BWI76_RS21645 are separated by 54 nucleotides BWI76_RS21645 and BWI76_RS21650 are separated by 85 nucleotides BWI76_RS21650 and BWI76_RS21655 are separated by 106 nucleotides BWI76_RS21655 and BWI76_RS21660 are separated by 78 nucleotides BWI76_RS21660 and BWI76_RS21665 are separated by 10 nucleotides BWI76_RS21665 and BWI76_RS21670 overlap by 4 nucleotides
BWI76_RS21640: BWI76_RS21640 - hypothetical protein, at 4,531,999 to 4,532,463
_RS21640
BWI76_RS21645: BWI76_RS21645 - hypothetical protein, at 4,532,518 to 4,532,877
_RS21645
BWI76_RS21650: BWI76_RS21650 - hypothetical protein, at 4,532,963 to 4,533,175
_RS21650
BWI76_RS21655: BWI76_RS21655 - hypothetical protein, at 4,533,282 to 4,534,103
_RS21655
BWI76_RS21660: BWI76_RS21660 - antirestriction protein, at 4,534,182 to 4,534,655
_RS21660
BWI76_RS21665: BWI76_RS21665 - hypothetical protein, at 4,534,666 to 4,534,899
_RS21665
BWI76_RS21670: BWI76_RS21670 - hypothetical protein, at 4,534,896 to 4,535,378
_RS21670
Position (kb)
4533
4534
4535 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4532.312 kb on + strand, within BWI76_RS21640 at 4532.330 kb on - strand, within BWI76_RS21640 at 4532.372 kb on + strand, within BWI76_RS21640 at 4532.372 kb on + strand, within BWI76_RS21640 at 4532.372 kb on + strand, within BWI76_RS21640 at 4532.380 kb on - strand, within BWI76_RS21640 at 4532.380 kb on - strand, within BWI76_RS21640 at 4532.405 kb on - strand, within BWI76_RS21640 at 4532.405 kb on - strand, within BWI76_RS21640 at 4532.433 kb on - strand at 4532.465 kb on - strand at 4532.535 kb on + strand at 4532.548 kb on - strand at 4532.557 kb on + strand, within BWI76_RS21645 at 4532.557 kb on + strand, within BWI76_RS21645 at 4532.563 kb on + strand, within BWI76_RS21645 at 4532.571 kb on - strand, within BWI76_RS21645 at 4532.587 kb on - strand, within BWI76_RS21645 at 4532.624 kb on - strand, within BWI76_RS21645 at 4532.626 kb on + strand, within BWI76_RS21645 at 4532.640 kb on + strand, within BWI76_RS21645 at 4532.655 kb on - strand, within BWI76_RS21645 at 4532.659 kb on + strand, within BWI76_RS21645 at 4532.662 kb on + strand, within BWI76_RS21645 at 4532.664 kb on + strand, within BWI76_RS21645 at 4532.664 kb on + strand, within BWI76_RS21645 at 4532.840 kb on + strand, within BWI76_RS21645 at 4532.850 kb on + strand at 4532.859 kb on + strand at 4532.859 kb on + strand at 4532.859 kb on + strand at 4532.859 kb on + strand at 4532.859 kb on + strand at 4532.859 kb on + strand at 4532.859 kb on + strand at 4532.859 kb on + strand at 4532.867 kb on - strand at 4532.867 kb on - strand at 4532.867 kb on - strand at 4532.945 kb on - strand at 4532.947 kb on + strand at 4532.947 kb on + strand at 4532.952 kb on + strand at 4532.995 kb on + strand, within BWI76_RS21650 at 4532.998 kb on - strand, within BWI76_RS21650 at 4533.010 kb on - strand, within BWI76_RS21650 at 4533.031 kb on - strand, within BWI76_RS21650 at 4533.090 kb on - strand, within BWI76_RS21650 at 4533.427 kb on + strand, within BWI76_RS21655 at 4533.427 kb on + strand, within BWI76_RS21655 at 4533.427 kb on + strand, within BWI76_RS21655 at 4533.454 kb on - strand, within BWI76_RS21655 at 4533.517 kb on + strand, within BWI76_RS21655 at 4533.523 kb on + strand, within BWI76_RS21655 at 4533.827 kb on + strand, within BWI76_RS21655 at 4533.827 kb on + strand, within BWI76_RS21655 at 4533.835 kb on - strand, within BWI76_RS21655 at 4533.927 kb on - strand, within BWI76_RS21655 at 4533.995 kb on + strand, within BWI76_RS21655 at 4533.995 kb on + strand, within BWI76_RS21655 at 4533.995 kb on + strand, within BWI76_RS21655 at 4534.003 kb on - strand, within BWI76_RS21655 at 4534.003 kb on - strand at 4534.387 kb on - strand, within BWI76_RS21660 at 4534.619 kb on + strand at 4534.882 kb on - strand at 4534.942 kb on + strand at 4534.963 kb on - strand, within BWI76_RS21670
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 4,532,312 + BWI76_RS21640 0.67 -4.7 4,532,330 - BWI76_RS21640 0.71 +0.4 4,532,372 + BWI76_RS21640 0.80 -3.1 4,532,372 + BWI76_RS21640 0.80 +0.9 4,532,372 + BWI76_RS21640 0.80 -0.4 4,532,380 - BWI76_RS21640 0.82 -2.8 4,532,380 - BWI76_RS21640 0.82 -1.5 4,532,405 - BWI76_RS21640 0.87 -0.1 4,532,405 - BWI76_RS21640 0.87 -0.8 4,532,433 - -0.2 4,532,465 - -0.8 4,532,535 + -0.7 4,532,548 - -2.8 4,532,557 + BWI76_RS21645 0.11 -0.4 4,532,557 + BWI76_RS21645 0.11 -1.6 4,532,563 + BWI76_RS21645 0.12 -1.1 4,532,571 - BWI76_RS21645 0.15 -2.1 4,532,587 - BWI76_RS21645 0.19 -3.3 4,532,624 - BWI76_RS21645 0.29 +0.4 4,532,626 + BWI76_RS21645 0.30 -0.3 4,532,640 + BWI76_RS21645 0.34 -0.9 4,532,655 - BWI76_RS21645 0.38 -0.5 4,532,659 + BWI76_RS21645 0.39 -2.4 4,532,662 + BWI76_RS21645 0.40 -3.6 4,532,664 + BWI76_RS21645 0.41 -2.1 4,532,664 + BWI76_RS21645 0.41 +0.2 4,532,840 + BWI76_RS21645 0.89 -0.3 4,532,850 + +0.9 4,532,859 + -3.3 4,532,859 + -1.5 4,532,859 + -1.6 4,532,859 + -0.9 4,532,859 + -1.3 4,532,859 + -1.6 4,532,859 + -0.1 4,532,859 + +1.0 4,532,867 - -0.1 4,532,867 - -2.9 4,532,867 - -0.4 4,532,945 - +0.5 4,532,947 + -1.3 4,532,947 + -0.7 4,532,952 + -2.0 4,532,995 + BWI76_RS21650 0.15 -1.2 4,532,998 - BWI76_RS21650 0.16 +0.0 4,533,010 - BWI76_RS21650 0.22 +0.1 4,533,031 - BWI76_RS21650 0.32 -1.5 4,533,090 - BWI76_RS21650 0.60 -2.5 4,533,427 + BWI76_RS21655 0.18 -0.0 4,533,427 + BWI76_RS21655 0.18 -2.5 4,533,427 + BWI76_RS21655 0.18 -1.0 4,533,454 - BWI76_RS21655 0.21 -1.6 4,533,517 + BWI76_RS21655 0.29 -4.8 4,533,523 + BWI76_RS21655 0.29 -1.9 4,533,827 + BWI76_RS21655 0.66 -0.7 4,533,827 + BWI76_RS21655 0.66 -1.5 4,533,835 - BWI76_RS21655 0.67 +0.3 4,533,927 - BWI76_RS21655 0.78 -3.2 4,533,995 + BWI76_RS21655 0.87 -0.4 4,533,995 + BWI76_RS21655 0.87 -0.5 4,533,995 + BWI76_RS21655 0.87 -0.8 4,534,003 - BWI76_RS21655 0.88 +1.3 4,534,003 - -1.1 4,534,387 - BWI76_RS21660 0.43 +0.6 4,534,619 + -1.5 4,534,882 - -1.7 4,534,942 + -0.9 4,534,963 - BWI76_RS21670 0.14 -3.2
Or see this region's nucleotide sequence