Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20960

Experiment: KLEB1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20955 and BWI76_RS20960 are separated by 9 nucleotidesBWI76_RS20960 and BWI76_RS20965 are separated by 265 nucleotides BWI76_RS20955: BWI76_RS20955 - 6-phospho-beta-glucosidase, at 4,379,771 to 4,381,201 _RS20955 BWI76_RS20960: BWI76_RS20960 - ROK family protein, at 4,381,211 to 4,382,104 _RS20960 BWI76_RS20965: BWI76_RS20965 - phosphoribosylformylglycinamidine cyclo-ligase, at 4,382,370 to 4,383,407 _RS20965 Position (kb) 4381 4382 4383Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4380.301 kb on - strand, within BWI76_RS20955at 4380.302 kb on - strand, within BWI76_RS20955at 4380.324 kb on + strand, within BWI76_RS20955at 4380.359 kb on + strand, within BWI76_RS20955at 4380.359 kb on + strand, within BWI76_RS20955at 4380.367 kb on - strand, within BWI76_RS20955at 4380.522 kb on - strand, within BWI76_RS20955at 4380.765 kb on - strand, within BWI76_RS20955at 4380.870 kb on + strand, within BWI76_RS20955at 4381.207 kb on + strandat 4381.291 kb on + strandat 4381.419 kb on + strand, within BWI76_RS20960at 4381.427 kb on - strand, within BWI76_RS20960at 4381.475 kb on - strand, within BWI76_RS20960at 4381.839 kb on - strand, within BWI76_RS20960at 4381.839 kb on - strand, within BWI76_RS20960at 4382.355 kb on + strandat 4382.628 kb on - strand, within BWI76_RS20965at 4382.639 kb on - strand, within BWI76_RS20965

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 10
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4,380,301 - BWI76_RS20955 0.37 -0.0
4,380,302 - BWI76_RS20955 0.37 -1.3
4,380,324 + BWI76_RS20955 0.39 -3.5
4,380,359 + BWI76_RS20955 0.41 -0.5
4,380,359 + BWI76_RS20955 0.41 +0.2
4,380,367 - BWI76_RS20955 0.42 +0.2
4,380,522 - BWI76_RS20955 0.52 -0.3
4,380,765 - BWI76_RS20955 0.69 +1.2
4,380,870 + BWI76_RS20955 0.77 -0.0
4,381,207 + -0.3
4,381,291 + +1.2
4,381,419 + BWI76_RS20960 0.23 -2.2
4,381,427 - BWI76_RS20960 0.24 -0.4
4,381,475 - BWI76_RS20960 0.30 -3.4
4,381,839 - BWI76_RS20960 0.70 -0.8
4,381,839 - BWI76_RS20960 0.70 -4.9
4,382,355 + -1.6
4,382,628 - BWI76_RS20965 0.25 -0.5
4,382,639 - BWI76_RS20965 0.26 -2.8

Or see this region's nucleotide sequence