Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19320

Experiment: KLEB1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19315 and BWI76_RS19320 overlap by 4 nucleotidesBWI76_RS19320 and BWI76_RS19325 are separated by 64 nucleotides BWI76_RS19315: BWI76_RS19315 - ABC transporter permease, at 4,023,203 to 4,024,240 _RS19315 BWI76_RS19320: BWI76_RS19320 - putative ABC transport system periplasmic binding protein, at 4,024,237 to 4,025,859 _RS19320 BWI76_RS19325: BWI76_RS19325 - mandelate racemase, at 4,025,924 to 4,027,078 _RS19325 Position (kb) 4024 4025 4026Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4023.369 kb on + strand, within BWI76_RS19315at 4023.410 kb on - strand, within BWI76_RS19315at 4023.486 kb on + strand, within BWI76_RS19315at 4023.579 kb on - strand, within BWI76_RS19315at 4023.784 kb on + strand, within BWI76_RS19315at 4023.872 kb on + strand, within BWI76_RS19315at 4024.108 kb on - strand, within BWI76_RS19315at 4024.165 kb on + strandat 4024.165 kb on + strandat 4024.165 kb on + strandat 4024.225 kb on + strandat 4024.400 kb on - strand, within BWI76_RS19320at 4024.404 kb on + strand, within BWI76_RS19320at 4024.504 kb on - strand, within BWI76_RS19320at 4025.386 kb on - strand, within BWI76_RS19320at 4025.646 kb on + strand, within BWI76_RS19320at 4026.801 kb on - strand, within BWI76_RS19325

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 10
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4,023,369 + BWI76_RS19315 0.16 +1.2
4,023,410 - BWI76_RS19315 0.20 +0.5
4,023,486 + BWI76_RS19315 0.27 -0.1
4,023,579 - BWI76_RS19315 0.36 -0.9
4,023,784 + BWI76_RS19315 0.56 -2.0
4,023,872 + BWI76_RS19315 0.64 -0.5
4,024,108 - BWI76_RS19315 0.87 -1.8
4,024,165 + -0.7
4,024,165 + -3.2
4,024,165 + -1.2
4,024,225 + +1.2
4,024,400 - BWI76_RS19320 0.10 -3.8
4,024,404 + BWI76_RS19320 0.10 -2.6
4,024,504 - BWI76_RS19320 0.16 -1.5
4,025,386 - BWI76_RS19320 0.71 -1.6
4,025,646 + BWI76_RS19320 0.87 -0.2
4,026,801 - BWI76_RS19325 0.76 -1.5

Or see this region's nucleotide sequence