Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14315 and BWI76_RS14320 are separated by 1 nucleotides BWI76_RS14320 and BWI76_RS14325 are separated by 7 nucleotides BWI76_RS14325 and BWI76_RS14330 are separated by 18 nucleotides
BWI76_RS14315: BWI76_RS14315 - MazG nucleotide pyrophosphohydrolase, at 2,991,554 to 2,991,892
_RS14315
BWI76_RS14320: BWI76_RS14320 - hypothetical protein, at 2,991,894 to 2,992,829
_RS14320
BWI76_RS14325: BWI76_RS14325 - maturase, at 2,992,837 to 2,992,957
_RS14325
BWI76_RS14330: BWI76_RS14330 - IS3 family transposase, at 2,992,976 to 2,993,797
_RS14330
Position (kb)
2992
2993 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2992.011 kb on + strand, within BWI76_RS14320 at 2992.114 kb on + strand, within BWI76_RS14320 at 2992.201 kb on + strand, within BWI76_RS14320 at 2992.293 kb on + strand at 2992.293 kb on + strand, within BWI76_RS14320 at 2992.395 kb on + strand, within BWI76_RS14320 at 2992.830 kb on + strand at 2992.894 kb on + strand, within BWI76_RS14325 at 2992.929 kb on - strand, within BWI76_RS14325 at 2992.932 kb on + strand, within BWI76_RS14325 at 2992.939 kb on + strand, within BWI76_RS14325 at 2992.945 kb on + strand, within BWI76_RS14325 at 2992.945 kb on + strand, within BWI76_RS14325 at 2992.945 kb on + strand, within BWI76_RS14325 at 2992.953 kb on + strand at 2992.989 kb on + strand at 2992.989 kb on + strand at 2993.024 kb on + strand at 2993.078 kb on - strand, within BWI76_RS14330 at 2993.080 kb on - strand at 2993.116 kb on + strand, within BWI76_RS14330 at 2993.159 kb on + strand, within BWI76_RS14330 at 2993.167 kb on - strand, within BWI76_RS14330 at 2993.191 kb on + strand, within BWI76_RS14330 at 2993.199 kb on - strand, within BWI76_RS14330 at 2993.224 kb on - strand, within BWI76_RS14330 at 2993.227 kb on + strand, within BWI76_RS14330 at 2993.227 kb on - strand, within BWI76_RS14330 at 2993.234 kb on - strand, within BWI76_RS14330 at 2993.235 kb on - strand, within BWI76_RS14330 at 2993.236 kb on + strand, within BWI76_RS14330 at 2993.236 kb on + strand, within BWI76_RS14330 at 2993.244 kb on - strand, within BWI76_RS14330 at 2993.317 kb on + strand, within BWI76_RS14330 at 2993.350 kb on + strand, within BWI76_RS14330 at 2993.418 kb on - strand at 2993.426 kb on - strand, within BWI76_RS14330 at 2993.467 kb on - strand, within BWI76_RS14330 at 2993.473 kb on + strand, within BWI76_RS14330 at 2993.749 kb on - strand at 2993.918 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 2,992,011 + BWI76_RS14320 0.12 +2.9 2,992,114 + BWI76_RS14320 0.24 +1.9 2,992,201 + BWI76_RS14320 0.33 -1.1 2,992,293 + +0.4 2,992,293 + BWI76_RS14320 0.43 +1.9 2,992,395 + BWI76_RS14320 0.54 -0.4 2,992,830 + -0.9 2,992,894 + BWI76_RS14325 0.47 -0.6 2,992,929 - BWI76_RS14325 0.76 -0.6 2,992,932 + BWI76_RS14325 0.79 -0.4 2,992,939 + BWI76_RS14325 0.84 -2.1 2,992,945 + BWI76_RS14325 0.89 -1.8 2,992,945 + BWI76_RS14325 0.89 -2.1 2,992,945 + BWI76_RS14325 0.89 -2.4 2,992,953 + -3.1 2,992,989 + -2.4 2,992,989 + -0.4 2,993,024 + -2.0 2,993,078 - BWI76_RS14330 0.12 -0.6 2,993,080 - +1.9 2,993,116 + BWI76_RS14330 0.17 +0.3 2,993,159 + BWI76_RS14330 0.22 -0.9 2,993,167 - BWI76_RS14330 0.23 +2.5 2,993,191 + BWI76_RS14330 0.26 +1.8 2,993,199 - BWI76_RS14330 0.27 +0.9 2,993,224 - BWI76_RS14330 0.30 -2.1 2,993,227 + BWI76_RS14330 0.31 +1.7 2,993,227 - BWI76_RS14330 0.31 +1.9 2,993,234 - BWI76_RS14330 0.31 -1.1 2,993,235 - BWI76_RS14330 0.32 -1.5 2,993,236 + BWI76_RS14330 0.32 -2.7 2,993,236 + BWI76_RS14330 0.32 -0.1 2,993,244 - BWI76_RS14330 0.33 -1.1 2,993,317 + BWI76_RS14330 0.41 -3.0 2,993,350 + BWI76_RS14330 0.45 -0.2 2,993,418 - -0.1 2,993,426 - BWI76_RS14330 0.55 -0.1 2,993,467 - BWI76_RS14330 0.60 -0.6 2,993,473 + BWI76_RS14330 0.60 -1.1 2,993,749 - -0.7 2,993,918 - +0.6
Or see this region's nucleotide sequence