Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09985 and BWI76_RS09990 are separated by 17 nucleotides BWI76_RS09990 and BWI76_RS09995 are separated by 122 nucleotides BWI76_RS09995 and BWI76_RS10000 are separated by 28 nucleotides BWI76_RS10000 and BWI76_RS10005 are separated by 154 nucleotides
BWI76_RS09985: BWI76_RS09985 - membrane protein, at 2,101,229 to 2,103,364
_RS09985
BWI76_RS09990: BWI76_RS09990 - hypothetical protein, at 2,103,382 to 2,103,828
_RS09990
BWI76_RS09995: BWI76_RS09995 - DNA helicase IV, at 2,103,951 to 2,106,005
_RS09995
BWI76_RS10000: BWI76_RS10000 - methylglyoxal synthase, at 2,106,034 to 2,106,492
_RS10000
BWI76_RS10005: BWI76_RS10005 - CoA-binding protein, at 2,106,647 to 2,107,060
_RS10005
Position (kb)
2103
2104
2105
2106
2107 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2103.116 kb on - strand, within BWI76_RS09985 at 2103.247 kb on + strand at 2103.452 kb on - strand, within BWI76_RS09990 at 2103.530 kb on - strand, within BWI76_RS09990 at 2103.679 kb on - strand, within BWI76_RS09990 at 2103.684 kb on - strand, within BWI76_RS09990 at 2103.684 kb on - strand, within BWI76_RS09990 at 2103.686 kb on - strand, within BWI76_RS09990 at 2103.710 kb on - strand, within BWI76_RS09990 at 2103.723 kb on - strand, within BWI76_RS09990 at 2103.724 kb on - strand, within BWI76_RS09990 at 2103.738 kb on + strand, within BWI76_RS09990 at 2103.803 kb on - strand at 2103.834 kb on - strand at 2103.838 kb on + strand at 2103.846 kb on - strand at 2103.859 kb on + strand at 2103.867 kb on - strand at 2104.006 kb on + strand at 2104.162 kb on + strand, within BWI76_RS09995 at 2104.205 kb on - strand, within BWI76_RS09995 at 2104.536 kb on - strand, within BWI76_RS09995 at 2104.760 kb on - strand, within BWI76_RS09995 at 2104.788 kb on - strand, within BWI76_RS09995 at 2104.921 kb on - strand, within BWI76_RS09995 at 2104.966 kb on - strand, within BWI76_RS09995 at 2105.437 kb on - strand, within BWI76_RS09995 at 2105.444 kb on + strand, within BWI76_RS09995 at 2105.444 kb on + strand, within BWI76_RS09995 at 2105.534 kb on - strand, within BWI76_RS09995 at 2105.879 kb on - strand at 2105.910 kb on + strand at 2105.910 kb on + strand at 2105.918 kb on - strand at 2105.918 kb on - strand at 2106.056 kb on - strand at 2106.128 kb on + strand, within BWI76_RS10000 at 2106.128 kb on + strand, within BWI76_RS10000 at 2106.197 kb on - strand, within BWI76_RS10000 at 2106.197 kb on - strand, within BWI76_RS10000 at 2106.269 kb on + strand, within BWI76_RS10000 at 2106.277 kb on - strand, within BWI76_RS10000 at 2106.323 kb on - strand, within BWI76_RS10000 at 2106.323 kb on - strand, within BWI76_RS10000 at 2106.323 kb on - strand, within BWI76_RS10000 at 2106.447 kb on + strand at 2106.455 kb on - strand at 2106.524 kb on - strand at 2106.582 kb on + strand at 2106.584 kb on - strand at 2106.626 kb on - strand at 2106.708 kb on - strand, within BWI76_RS10005 at 2106.708 kb on - strand, within BWI76_RS10005 at 2106.720 kb on - strand, within BWI76_RS10005 at 2106.772 kb on + strand, within BWI76_RS10005 at 2106.801 kb on + strand, within BWI76_RS10005 at 2106.804 kb on + strand, within BWI76_RS10005 at 2106.817 kb on - strand, within BWI76_RS10005 at 2106.882 kb on + strand, within BWI76_RS10005 at 2106.905 kb on + strand, within BWI76_RS10005 at 2106.949 kb on + strand, within BWI76_RS10005 at 2106.985 kb on - strand, within BWI76_RS10005 at 2106.998 kb on - strand, within BWI76_RS10005
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 2,103,116 - BWI76_RS09985 0.88 -0.8 2,103,247 + -1.7 2,103,452 - BWI76_RS09990 0.16 +0.0 2,103,530 - BWI76_RS09990 0.33 -2.8 2,103,679 - BWI76_RS09990 0.66 +2.3 2,103,684 - BWI76_RS09990 0.68 -0.9 2,103,684 - BWI76_RS09990 0.68 -0.9 2,103,686 - BWI76_RS09990 0.68 -1.1 2,103,710 - BWI76_RS09990 0.73 +0.2 2,103,723 - BWI76_RS09990 0.76 -0.6 2,103,724 - BWI76_RS09990 0.77 -2.4 2,103,738 + BWI76_RS09990 0.80 -0.7 2,103,803 - -2.7 2,103,834 - +0.2 2,103,838 + -2.2 2,103,846 - -1.3 2,103,859 + -0.6 2,103,867 - +0.4 2,104,006 + -2.0 2,104,162 + BWI76_RS09995 0.10 -3.1 2,104,205 - BWI76_RS09995 0.12 -1.4 2,104,536 - BWI76_RS09995 0.28 -3.9 2,104,760 - BWI76_RS09995 0.39 -2.5 2,104,788 - BWI76_RS09995 0.41 +0.2 2,104,921 - BWI76_RS09995 0.47 +0.5 2,104,966 - BWI76_RS09995 0.49 -2.0 2,105,437 - BWI76_RS09995 0.72 -1.2 2,105,444 + BWI76_RS09995 0.73 -0.3 2,105,444 + BWI76_RS09995 0.73 -1.4 2,105,534 - BWI76_RS09995 0.77 -2.0 2,105,879 - -3.2 2,105,910 + -1.0 2,105,910 + +0.3 2,105,918 - -3.5 2,105,918 - -0.7 2,106,056 - -0.6 2,106,128 + BWI76_RS10000 0.20 -0.2 2,106,128 + BWI76_RS10000 0.20 -0.3 2,106,197 - BWI76_RS10000 0.36 -1.0 2,106,197 - BWI76_RS10000 0.36 -1.2 2,106,269 + BWI76_RS10000 0.51 +0.6 2,106,277 - BWI76_RS10000 0.53 -2.8 2,106,323 - BWI76_RS10000 0.63 -1.0 2,106,323 - BWI76_RS10000 0.63 +2.0 2,106,323 - BWI76_RS10000 0.63 +1.3 2,106,447 + +0.9 2,106,455 - -0.3 2,106,524 - -0.9 2,106,582 + -0.4 2,106,584 - -1.1 2,106,626 - -0.5 2,106,708 - BWI76_RS10005 0.15 +0.1 2,106,708 - BWI76_RS10005 0.15 +0.7 2,106,720 - BWI76_RS10005 0.18 -0.4 2,106,772 + BWI76_RS10005 0.30 -0.2 2,106,801 + BWI76_RS10005 0.37 +0.3 2,106,804 + BWI76_RS10005 0.38 -1.0 2,106,817 - BWI76_RS10005 0.41 -0.8 2,106,882 + BWI76_RS10005 0.57 -0.8 2,106,905 + BWI76_RS10005 0.62 +0.2 2,106,949 + BWI76_RS10005 0.73 +0.6 2,106,985 - BWI76_RS10005 0.82 +0.2 2,106,998 - BWI76_RS10005 0.85 +1.3
Or see this region's nucleotide sequence