Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08955 and BWI76_RS08960 overlap by 6 nucleotides BWI76_RS08960 and BWI76_RS08965 are separated by 110 nucleotides BWI76_RS08965 and BWI76_RS08970 overlap by 4 nucleotides
BWI76_RS08955: BWI76_RS08955 - 23S rRNA (adenine(1618)-N(6))-methyltransferase, at 1,886,653 to 1,887,567
_RS08955
BWI76_RS08960: BWI76_RS08960 - mechanosensitive channel protein, at 1,887,562 to 1,889,772
_RS08960
BWI76_RS08965: BWI76_RS08965 - glutamine ABC transporter ATP-binding protein, at 1,889,883 to 1,890,605
_RS08965
BWI76_RS08970: BWI76_RS08970 - glutamine ABC transporter permease GlnP, at 1,890,602 to 1,891,261
_RS08970
Position (kb) 1887
1888
1889
1890 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1886.682 kb on + strand at 1886.710 kb on - strand at 1886.838 kb on + strand, within BWI76_RS08955 at 1886.856 kb on - strand, within BWI76_RS08955 at 1887.157 kb on - strand, within BWI76_RS08955 at 1887.196 kb on + strand, within BWI76_RS08955 at 1887.209 kb on - strand, within BWI76_RS08955 at 1887.274 kb on - strand, within BWI76_RS08955 at 1887.274 kb on - strand, within BWI76_RS08955 at 1887.274 kb on - strand, within BWI76_RS08955 at 1887.446 kb on - strand, within BWI76_RS08955 at 1888.058 kb on + strand, within BWI76_RS08960 at 1888.343 kb on - strand, within BWI76_RS08960 at 1888.343 kb on - strand, within BWI76_RS08960 at 1888.640 kb on + strand, within BWI76_RS08960 at 1888.749 kb on + strand, within BWI76_RS08960 at 1888.839 kb on + strand, within BWI76_RS08960 at 1888.896 kb on - strand, within BWI76_RS08960 at 1889.471 kb on + strand, within BWI76_RS08960 at 1889.479 kb on - strand, within BWI76_RS08960 at 1889.803 kb on + strand at 1889.912 kb on + strand at 1890.342 kb on + strand, within BWI76_RS08965 at 1890.421 kb on - strand, within BWI76_RS08965 at 1890.461 kb on + strand, within BWI76_RS08965 at 1890.469 kb on - strand, within BWI76_RS08965 at 1890.474 kb on + strand, within BWI76_RS08965 at 1890.487 kb on - strand, within BWI76_RS08965 at 1890.645 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 1,886,682 + -1.3 1,886,710 - +1.6 1,886,838 + BWI76_RS08955 0.20 +0.1 1,886,856 - BWI76_RS08955 0.22 +0.3 1,887,157 - BWI76_RS08955 0.55 +0.2 1,887,196 + BWI76_RS08955 0.59 -2.1 1,887,209 - BWI76_RS08955 0.61 +0.8 1,887,274 - BWI76_RS08955 0.68 -0.4 1,887,274 - BWI76_RS08955 0.68 +1.5 1,887,274 - BWI76_RS08955 0.68 +1.6 1,887,446 - BWI76_RS08955 0.87 -1.1 1,888,058 + BWI76_RS08960 0.22 +0.4 1,888,343 - BWI76_RS08960 0.35 +0.5 1,888,343 - BWI76_RS08960 0.35 -2.9 1,888,640 + BWI76_RS08960 0.49 -0.1 1,888,749 + BWI76_RS08960 0.54 -2.9 1,888,839 + BWI76_RS08960 0.58 +0.9 1,888,896 - BWI76_RS08960 0.60 -3.4 1,889,471 + BWI76_RS08960 0.86 +1.2 1,889,479 - BWI76_RS08960 0.87 +0.1 1,889,803 + +1.3 1,889,912 + -1.1 1,890,342 + BWI76_RS08965 0.63 +0.9 1,890,421 - BWI76_RS08965 0.74 +1.8 1,890,461 + BWI76_RS08965 0.80 +0.3 1,890,469 - BWI76_RS08965 0.81 -0.0 1,890,474 + BWI76_RS08965 0.82 +0.9 1,890,487 - BWI76_RS08965 0.84 -0.5 1,890,645 - +0.4
Or see this region's nucleotide sequence