Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08735

Experiment: KLEB1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08725 and BWI76_RS08730 are separated by 53 nucleotidesBWI76_RS08730 and BWI76_RS08735 are separated by 143 nucleotidesBWI76_RS08735 and BWI76_RS08740 overlap by 4 nucleotides BWI76_RS08725: BWI76_RS08725 - Formiminoglutamase (EC 3.5.3.8) (from data), at 1,837,820 to 1,838,776 _RS08725 BWI76_RS08730: BWI76_RS08730 - histidine utilization repressor, at 1,838,830 to 1,839,555 _RS08730 BWI76_RS08735: BWI76_RS08735 - Urocanate hydratase (EC 4.2.1.49) (from data), at 1,839,699 to 1,841,387 _RS08735 BWI76_RS08740: BWI76_RS08740 - Histidine ammonia-lyase (EC 4.3.1.3) (from data), at 1,841,384 to 1,842,907 _RS08740 Position (kb) 1839 1840 1841 1842Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1839.414 kb on - strand, within BWI76_RS08730at 1839.414 kb on - strand, within BWI76_RS08730at 1839.482 kb on - strand, within BWI76_RS08730at 1839.640 kb on - strandat 1840.194 kb on - strand, within BWI76_RS08735at 1840.386 kb on + strand, within BWI76_RS08735at 1840.558 kb on - strand, within BWI76_RS08735at 1840.895 kb on + strand, within BWI76_RS08735at 1841.111 kb on + strand, within BWI76_RS08735at 1841.181 kb on - strand, within BWI76_RS08735at 1841.844 kb on + strand, within BWI76_RS08740at 1841.968 kb on + strand, within BWI76_RS08740at 1842.064 kb on - strand, within BWI76_RS08740at 1842.136 kb on + strandat 1842.263 kb on + strand, within BWI76_RS08740at 1842.272 kb on - strand, within BWI76_RS08740

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 10
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1,839,414 - BWI76_RS08730 0.80 -1.4
1,839,414 - BWI76_RS08730 0.80 -0.9
1,839,482 - BWI76_RS08730 0.90 -1.4
1,839,640 - +1.5
1,840,194 - BWI76_RS08735 0.29 -1.1
1,840,386 + BWI76_RS08735 0.41 +0.4
1,840,558 - BWI76_RS08735 0.51 -3.7
1,840,895 + BWI76_RS08735 0.71 -4.1
1,841,111 + BWI76_RS08735 0.84 -3.6
1,841,181 - BWI76_RS08735 0.88 +0.1
1,841,844 + BWI76_RS08740 0.30 -0.7
1,841,968 + BWI76_RS08740 0.38 +0.2
1,842,064 - BWI76_RS08740 0.45 -0.4
1,842,136 + -0.7
1,842,263 + BWI76_RS08740 0.58 +0.1
1,842,272 - BWI76_RS08740 0.58 +0.3

Or see this region's nucleotide sequence