Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08290

Experiment: KLEB1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08280 and BWI76_RS08285 are separated by 184 nucleotidesBWI76_RS08285 and BWI76_RS08290 are separated by 32 nucleotidesBWI76_RS08290 and BWI76_RS08295 are separated by 56 nucleotidesBWI76_RS08295 and BWI76_RS08300 are separated by 3 nucleotides BWI76_RS08280: BWI76_RS08280 - acyl-CoA esterase, at 1,751,986 to 1,752,759 _RS08280 BWI76_RS08285: BWI76_RS08285 - replication initiation regulator SeqA, at 1,752,944 to 1,753,492 _RS08285 BWI76_RS08290: BWI76_RS08290 - phosphoglucomutase, alpha-D-glucose phosphate-specific, at 1,753,525 to 1,755,165 _RS08290 BWI76_RS08295: BWI76_RS08295 - hypothetical protein, at 1,755,222 to 1,755,641 _RS08295 BWI76_RS08300: BWI76_RS08300 - DNA-binding response regulator, at 1,755,645 to 1,756,322 _RS08300 Position (kb) 1753 1754 1755 1756Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1752.589 kb on + strand, within BWI76_RS08280at 1752.605 kb on - strand, within BWI76_RS08280at 1752.857 kb on + strandat 1752.862 kb on + strandat 1752.936 kb on + strandat 1753.237 kb on + strand, within BWI76_RS08285at 1753.371 kb on - strand, within BWI76_RS08285at 1755.272 kb on + strand, within BWI76_RS08295at 1755.275 kb on - strand, within BWI76_RS08295at 1755.281 kb on + strand, within BWI76_RS08295at 1755.294 kb on - strand, within BWI76_RS08295at 1755.329 kb on - strand, within BWI76_RS08295at 1755.329 kb on - strand, within BWI76_RS08295at 1755.393 kb on - strand, within BWI76_RS08295at 1755.418 kb on - strand, within BWI76_RS08295at 1755.624 kb on - strandat 1756.151 kb on - strand, within BWI76_RS08300

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 10
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1,752,589 + BWI76_RS08280 0.78 +2.8
1,752,605 - BWI76_RS08280 0.80 -0.2
1,752,857 + -2.6
1,752,862 + +1.0
1,752,936 + -0.4
1,753,237 + BWI76_RS08285 0.53 -0.6
1,753,371 - BWI76_RS08285 0.78 +0.7
1,755,272 + BWI76_RS08295 0.12 +0.1
1,755,275 - BWI76_RS08295 0.13 -0.9
1,755,281 + BWI76_RS08295 0.14 +0.7
1,755,294 - BWI76_RS08295 0.17 -1.8
1,755,329 - BWI76_RS08295 0.25 -0.0
1,755,329 - BWI76_RS08295 0.25 +2.4
1,755,393 - BWI76_RS08295 0.41 +0.4
1,755,418 - BWI76_RS08295 0.47 +1.4
1,755,624 - -0.2
1,756,151 - BWI76_RS08300 0.75 +1.1

Or see this region's nucleotide sequence