Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08250

Experiment: KLEB1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08245 and BWI76_RS08250 are separated by 52 nucleotidesBWI76_RS08250 and BWI76_RS08255 overlap by 14 nucleotides BWI76_RS08245: BWI76_RS08245 - MFS transporter, at 1,745,104 to 1,746,399 _RS08245 BWI76_RS08250: BWI76_RS08250 - tricarballylate utilization protein B, at 1,746,452 to 1,747,591 _RS08250 BWI76_RS08255: BWI76_RS08255 - tricarballylate dehydrogenase, at 1,747,578 to 1,748,981 _RS08255 Position (kb) 1746 1747 1748Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1745.456 kb on + strand, within BWI76_RS08245at 1745.570 kb on + strand, within BWI76_RS08245at 1745.754 kb on + strandat 1746.007 kb on + strandat 1746.365 kb on - strandat 1746.439 kb on + strandat 1746.452 kb on - strandat 1746.739 kb on + strand, within BWI76_RS08250at 1746.936 kb on - strand, within BWI76_RS08250at 1746.936 kb on - strand, within BWI76_RS08250at 1747.282 kb on - strand, within BWI76_RS08250at 1747.506 kb on + strandat 1747.605 kb on - strandat 1748.208 kb on + strand, within BWI76_RS08255at 1748.216 kb on - strand, within BWI76_RS08255at 1748.299 kb on + strand, within BWI76_RS08255at 1748.336 kb on + strand, within BWI76_RS08255at 1748.344 kb on - strand, within BWI76_RS08255at 1748.584 kb on + strand, within BWI76_RS08255

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 10
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1,745,456 + BWI76_RS08245 0.27 -0.9
1,745,570 + BWI76_RS08245 0.36 -3.2
1,745,754 + -1.9
1,746,007 + -0.3
1,746,365 - +1.7
1,746,439 + +0.2
1,746,452 - -0.8
1,746,739 + BWI76_RS08250 0.25 -3.9
1,746,936 - BWI76_RS08250 0.42 -4.0
1,746,936 - BWI76_RS08250 0.42 -2.5
1,747,282 - BWI76_RS08250 0.73 -3.3
1,747,506 + +1.2
1,747,605 - +0.2
1,748,208 + BWI76_RS08255 0.45 -0.1
1,748,216 - BWI76_RS08255 0.45 +0.8
1,748,299 + BWI76_RS08255 0.51 +1.0
1,748,336 + BWI76_RS08255 0.54 -0.1
1,748,344 - BWI76_RS08255 0.55 +0.9
1,748,584 + BWI76_RS08255 0.72 +0.6

Or see this region's nucleotide sequence