Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05950

Experiment: KLEB1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05940 and BWI76_RS05945 are separated by 27 nucleotidesBWI76_RS05945 and BWI76_RS05950 overlap by 11 nucleotidesBWI76_RS05950 and BWI76_RS05955 overlap by 4 nucleotidesBWI76_RS05955 and BWI76_RS05960 are separated by 13 nucleotides BWI76_RS05940: BWI76_RS05940 - hypothetical protein, at 1,249,636 to 1,250,340 _RS05940 BWI76_RS05945: BWI76_RS05945 - amino acid ABC transporter permease, at 1,250,368 to 1,251,051 _RS05945 BWI76_RS05950: BWI76_RS05950 - polar amino acid ABC transporter inner membrane subunit, at 1,251,041 to 1,251,793 _RS05950 BWI76_RS05955: BWI76_RS05955 - ABC transporter, at 1,251,790 to 1,252,608 _RS05955 BWI76_RS05960: BWI76_RS05960 - cysteine transport system periplasmic binding protein, at 1,252,622 to 1,253,431 _RS05960 Position (kb) 1251 1252Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1250.122 kb on + strand, within BWI76_RS05940at 1250.176 kb on - strand, within BWI76_RS05940at 1250.291 kb on - strandat 1250.293 kb on - strandat 1250.317 kb on + strandat 1250.427 kb on - strandat 1250.465 kb on + strand, within BWI76_RS05945at 1250.465 kb on + strand, within BWI76_RS05945at 1250.473 kb on - strand, within BWI76_RS05945at 1250.554 kb on - strand, within BWI76_RS05945at 1250.596 kb on - strand, within BWI76_RS05945at 1250.729 kb on + strand, within BWI76_RS05945at 1250.929 kb on - strand, within BWI76_RS05945at 1251.378 kb on - strand, within BWI76_RS05950at 1252.054 kb on - strand, within BWI76_RS05955at 1252.243 kb on + strand, within BWI76_RS05955at 1252.419 kb on - strand, within BWI76_RS05955at 1252.780 kb on + strand, within BWI76_RS05960

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 10
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1,250,122 + BWI76_RS05940 0.69 +0.2
1,250,176 - BWI76_RS05940 0.77 -1.2
1,250,291 - +2.1
1,250,293 - +0.6
1,250,317 + -1.1
1,250,427 - +0.2
1,250,465 + BWI76_RS05945 0.14 +1.4
1,250,465 + BWI76_RS05945 0.14 -0.0
1,250,473 - BWI76_RS05945 0.15 +1.2
1,250,554 - BWI76_RS05945 0.27 -3.2
1,250,596 - BWI76_RS05945 0.33 -2.7
1,250,729 + BWI76_RS05945 0.53 -4.2
1,250,929 - BWI76_RS05945 0.82 -2.8
1,251,378 - BWI76_RS05950 0.45 -1.7
1,252,054 - BWI76_RS05955 0.32 -4.1
1,252,243 + BWI76_RS05955 0.55 +0.4
1,252,419 - BWI76_RS05955 0.77 -1.3
1,252,780 + BWI76_RS05960 0.20 +0.4

Or see this region's nucleotide sequence