Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03520 and BWI76_RS03525 are separated by 277 nucleotides BWI76_RS03525 and BWI76_RS03530 are separated by 206 nucleotides
BWI76_RS03520: BWI76_RS03520 - diguanylate cyclase, at 729,781 to 731,178
_RS03520
BWI76_RS03525: BWI76_RS03525 - tryptophanyl-tRNA synthetase, at 731,456 to 732,463
_RS03525
BWI76_RS03530: BWI76_RS03530 - tryptophan permease, at 732,670 to 733,926
_RS03530
Position (kb) 731
732
733 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 730.531 kb on + strand, within BWI76_RS03520 at 730.535 kb on - strand, within BWI76_RS03520 at 730.536 kb on + strand at 730.542 kb on - strand, within BWI76_RS03520 at 730.544 kb on - strand, within BWI76_RS03520 at 730.544 kb on - strand, within BWI76_RS03520 at 730.545 kb on - strand, within BWI76_RS03520 at 730.555 kb on - strand, within BWI76_RS03520 at 730.594 kb on + strand, within BWI76_RS03520 at 730.602 kb on - strand, within BWI76_RS03520 at 730.614 kb on - strand, within BWI76_RS03520 at 730.624 kb on - strand, within BWI76_RS03520 at 730.638 kb on + strand, within BWI76_RS03520 at 730.638 kb on + strand, within BWI76_RS03520 at 730.642 kb on + strand, within BWI76_RS03520 at 730.642 kb on + strand, within BWI76_RS03520 at 730.642 kb on + strand, within BWI76_RS03520 at 730.646 kb on - strand, within BWI76_RS03520 at 730.648 kb on + strand, within BWI76_RS03520 at 730.648 kb on + strand, within BWI76_RS03520 at 730.650 kb on + strand, within BWI76_RS03520 at 730.650 kb on - strand, within BWI76_RS03520 at 730.656 kb on - strand, within BWI76_RS03520 at 730.666 kb on - strand, within BWI76_RS03520 at 730.699 kb on + strand, within BWI76_RS03520 at 730.723 kb on - strand, within BWI76_RS03520 at 730.757 kb on + strand at 731.147 kb on + strand at 731.223 kb on - strand at 731.247 kb on - strand at 731.326 kb on - strand at 731.381 kb on - strand at 731.447 kb on - strand at 731.454 kb on + strand at 731.462 kb on - strand at 731.548 kb on - strand at 731.614 kb on + strand, within BWI76_RS03525 at 731.709 kb on + strand, within BWI76_RS03525 at 731.745 kb on + strand, within BWI76_RS03525 at 731.920 kb on - strand, within BWI76_RS03525 at 731.929 kb on + strand, within BWI76_RS03525 at 732.106 kb on - strand, within BWI76_RS03525 at 732.437 kb on + strand at 732.532 kb on + strand at 732.532 kb on + strand at 732.579 kb on + strand at 732.690 kb on + strand at 732.690 kb on + strand at 732.698 kb on - strand at 732.798 kb on - strand, within BWI76_RS03530 at 732.822 kb on - strand, within BWI76_RS03530 at 732.839 kb on - strand, within BWI76_RS03530 at 732.901 kb on - strand, within BWI76_RS03530 at 732.949 kb on - strand, within BWI76_RS03530 at 733.002 kb on + strand, within BWI76_RS03530 at 733.067 kb on - strand, within BWI76_RS03530 at 733.087 kb on - strand, within BWI76_RS03530 at 733.099 kb on + strand, within BWI76_RS03530 at 733.208 kb on + strand, within BWI76_RS03530 at 733.339 kb on + strand, within BWI76_RS03530 at 733.339 kb on + strand, within BWI76_RS03530 at 733.359 kb on + strand, within BWI76_RS03530 at 733.404 kb on - strand, within BWI76_RS03530 at 733.444 kb on + strand, within BWI76_RS03530
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 730,531 + BWI76_RS03520 0.54 -3.5 730,535 - BWI76_RS03520 0.54 -2.6 730,536 + +1.4 730,542 - BWI76_RS03520 0.54 -1.3 730,544 - BWI76_RS03520 0.55 +0.6 730,544 - BWI76_RS03520 0.55 -1.9 730,545 - BWI76_RS03520 0.55 +2.4 730,555 - BWI76_RS03520 0.55 -2.9 730,594 + BWI76_RS03520 0.58 +0.7 730,602 - BWI76_RS03520 0.59 +1.6 730,614 - BWI76_RS03520 0.60 -1.6 730,624 - BWI76_RS03520 0.60 +0.4 730,638 + BWI76_RS03520 0.61 +0.1 730,638 + BWI76_RS03520 0.61 +0.8 730,642 + BWI76_RS03520 0.62 +0.1 730,642 + BWI76_RS03520 0.62 +1.7 730,642 + BWI76_RS03520 0.62 -2.1 730,646 - BWI76_RS03520 0.62 +0.9 730,648 + BWI76_RS03520 0.62 -1.2 730,648 + BWI76_RS03520 0.62 +0.4 730,650 + BWI76_RS03520 0.62 +1.4 730,650 - BWI76_RS03520 0.62 -0.9 730,656 - BWI76_RS03520 0.63 +0.1 730,666 - BWI76_RS03520 0.63 -0.6 730,699 + BWI76_RS03520 0.66 -3.7 730,723 - BWI76_RS03520 0.67 -0.2 730,757 + -1.8 731,147 + +0.3 731,223 - -0.5 731,247 - -0.6 731,326 - -3.7 731,381 - -2.5 731,447 - +0.1 731,454 + +0.4 731,462 - +0.9 731,548 - -0.1 731,614 + BWI76_RS03525 0.16 +1.5 731,709 + BWI76_RS03525 0.25 +2.5 731,745 + BWI76_RS03525 0.29 -1.2 731,920 - BWI76_RS03525 0.46 +0.3 731,929 + BWI76_RS03525 0.47 +1.3 732,106 - BWI76_RS03525 0.64 -0.4 732,437 + -0.5 732,532 + +0.1 732,532 + -2.2 732,579 + -0.2 732,690 + +1.6 732,690 + -0.1 732,698 - -0.1 732,798 - BWI76_RS03530 0.10 -0.8 732,822 - BWI76_RS03530 0.12 +0.5 732,839 - BWI76_RS03530 0.13 +1.6 732,901 - BWI76_RS03530 0.18 +0.6 732,949 - BWI76_RS03530 0.22 +2.8 733,002 + BWI76_RS03530 0.26 -2.2 733,067 - BWI76_RS03530 0.32 -0.8 733,087 - BWI76_RS03530 0.33 +1.1 733,099 + BWI76_RS03530 0.34 +0.3 733,208 + BWI76_RS03530 0.43 -0.3 733,339 + BWI76_RS03530 0.53 +1.6 733,339 + BWI76_RS03530 0.53 +0.4 733,359 + BWI76_RS03530 0.55 -1.6 733,404 - BWI76_RS03530 0.58 +0.2 733,444 + BWI76_RS03530 0.62 +2.0
Or see this region's nucleotide sequence