Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01225 and BWI76_RS01230 are separated by 43 nucleotides BWI76_RS01230 and BWI76_RS01235 are separated by 49 nucleotides BWI76_RS01235 and BWI76_RS01240 are separated by 155 nucleotides
BWI76_RS01225: BWI76_RS01225 - magnesium transporter CorA, at 251,098 to 252,048
_RS01225
BWI76_RS01230: BWI76_RS01230 - chloramphenicol resistance permease RarD, at 252,092 to 252,985
_RS01230
BWI76_RS01235: BWI76_RS01235 - hypothetical protein, at 253,035 to 253,499
_RS01235
BWI76_RS01240: BWI76_RS01240 - phospholipase A, at 253,655 to 254,527
_RS01240
Position (kb) 252
253 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 251.188 kb on - strand at 251.236 kb on + strand, within BWI76_RS01225 at 251.255 kb on + strand, within BWI76_RS01225 at 251.298 kb on + strand, within BWI76_RS01225 at 251.366 kb on + strand, within BWI76_RS01225 at 251.414 kb on + strand, within BWI76_RS01225 at 251.547 kb on + strand, within BWI76_RS01225 at 251.929 kb on - strand, within BWI76_RS01225 at 251.940 kb on + strand, within BWI76_RS01225 at 252.165 kb on - strand at 252.262 kb on + strand, within BWI76_RS01230 at 252.265 kb on - strand, within BWI76_RS01230 at 252.287 kb on - strand, within BWI76_RS01230 at 252.549 kb on + strand, within BWI76_RS01230 at 252.557 kb on - strand, within BWI76_RS01230 at 252.557 kb on - strand, within BWI76_RS01230 at 253.077 kb on - strand at 253.154 kb on + strand, within BWI76_RS01235 at 253.273 kb on + strand, within BWI76_RS01235 at 253.538 kb on - strand at 253.615 kb on - strand at 253.657 kb on + strand at 253.664 kb on + strand at 253.664 kb on + strand at 253.672 kb on - strand at 253.672 kb on - strand at 253.743 kb on + strand, within BWI76_RS01240 at 253.815 kb on + strand, within BWI76_RS01240 at 253.823 kb on - strand, within BWI76_RS01240 at 253.939 kb on - strand, within BWI76_RS01240
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 251,188 - -0.6 251,236 + BWI76_RS01225 0.15 -0.2 251,255 + BWI76_RS01225 0.17 -0.7 251,298 + BWI76_RS01225 0.21 +1.0 251,366 + BWI76_RS01225 0.28 +1.0 251,414 + BWI76_RS01225 0.33 -0.0 251,547 + BWI76_RS01225 0.47 +1.9 251,929 - BWI76_RS01225 0.87 +0.6 251,940 + BWI76_RS01225 0.89 -0.3 252,165 - +1.4 252,262 + BWI76_RS01230 0.19 +0.2 252,265 - BWI76_RS01230 0.19 +0.2 252,287 - BWI76_RS01230 0.22 +0.7 252,549 + BWI76_RS01230 0.51 +1.2 252,557 - BWI76_RS01230 0.52 +0.3 252,557 - BWI76_RS01230 0.52 +0.2 253,077 - -0.2 253,154 + BWI76_RS01235 0.26 -1.0 253,273 + BWI76_RS01235 0.51 -0.4 253,538 - -3.6 253,615 - -0.4 253,657 + +0.2 253,664 + +0.6 253,664 + +1.6 253,672 - +0.7 253,672 - +1.2 253,743 + BWI76_RS01240 0.10 +0.9 253,815 + BWI76_RS01240 0.18 +1.7 253,823 - BWI76_RS01240 0.19 -0.3 253,939 - BWI76_RS01240 0.33 +1.4
Or see this region's nucleotide sequence