Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00990 and BWI76_RS00995 are separated by 100 nucleotides BWI76_RS00995 and BWI76_RS01000 are separated by 52 nucleotides BWI76_RS01000 and BWI76_RS01005 are separated by 194 nucleotides BWI76_RS01005 and BWI76_RS01010 are separated by 24 nucleotides
BWI76_RS00990: BWI76_RS00990 - ketol-acid reductoisomerase, at 202,804 to 204,279
_RS00990
BWI76_RS00995: BWI76_RS00995 - peptidyl-prolyl cis-trans isomerase, at 204,380 to 204,661
_RS00995
BWI76_RS01000: BWI76_RS01000 - peptidyl-prolyl cis-trans isomerase, at 204,714 to 204,995
_RS01000
BWI76_RS01005: BWI76_RS01005 - CarD family transcriptional regulator, at 205,190 to 205,894
_RS01005
BWI76_RS01010: BWI76_RS01010 - uridine phosphorylase, at 205,919 to 206,668
_RS01010
Position (kb) 204
205 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 204.075 kb on - strand at 204.167 kb on + strand at 204.305 kb on + strand at 204.306 kb on + strand at 204.330 kb on + strand at 204.458 kb on + strand, within BWI76_RS00995 at 204.466 kb on - strand, within BWI76_RS00995 at 204.496 kb on - strand, within BWI76_RS00995 at 204.720 kb on - strand at 204.783 kb on - strand, within BWI76_RS01000 at 204.800 kb on - strand at 204.819 kb on + strand, within BWI76_RS01000 at 204.906 kb on + strand, within BWI76_RS01000 at 204.936 kb on - strand, within BWI76_RS01000 at 204.948 kb on + strand, within BWI76_RS01000 at 204.977 kb on - strand at 204.977 kb on - strand at 205.053 kb on + strand at 205.053 kb on + strand at 205.145 kb on + strand at 205.163 kb on + strand at 205.163 kb on + strand at 205.165 kb on + strand at 205.165 kb on + strand at 205.166 kb on - strand at 205.240 kb on + strand at 205.414 kb on + strand, within BWI76_RS01005 at 205.423 kb on - strand, within BWI76_RS01005 at 205.529 kb on + strand, within BWI76_RS01005 at 205.561 kb on - strand, within BWI76_RS01005 at 205.632 kb on - strand, within BWI76_RS01005 at 205.812 kb on - strand, within BWI76_RS01005 at 205.812 kb on - strand, within BWI76_RS01005 at 205.938 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 204,075 - +3.4 204,167 + -0.4 204,305 + -0.6 204,306 + -1.4 204,330 + -3.8 204,458 + BWI76_RS00995 0.28 +0.6 204,466 - BWI76_RS00995 0.30 -0.7 204,496 - BWI76_RS00995 0.41 -1.1 204,720 - -0.7 204,783 - BWI76_RS01000 0.24 -4.7 204,800 - -0.8 204,819 + BWI76_RS01000 0.37 +1.2 204,906 + BWI76_RS01000 0.68 +1.2 204,936 - BWI76_RS01000 0.79 +1.0 204,948 + BWI76_RS01000 0.83 -3.1 204,977 - +0.6 204,977 - -3.2 205,053 + -0.4 205,053 + +1.1 205,145 + -1.6 205,163 + -2.9 205,163 + -1.4 205,165 + +0.2 205,165 + -1.6 205,166 - -0.4 205,240 + -1.6 205,414 + BWI76_RS01005 0.32 +5.6 205,423 - BWI76_RS01005 0.33 -0.2 205,529 + BWI76_RS01005 0.48 -0.4 205,561 - BWI76_RS01005 0.53 +0.6 205,632 - BWI76_RS01005 0.63 +0.1 205,812 - BWI76_RS01005 0.88 -0.3 205,812 - BWI76_RS01005 0.88 -3.8 205,938 - +1.2
Or see this region's nucleotide sequence