Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24925 and BWI76_RS24930 are separated by 9 nucleotides BWI76_RS24930 and BWI76_RS24935 are separated by 207 nucleotides BWI76_RS24935 and BWI76_RS24940 are separated by 72 nucleotides BWI76_RS24940 and BWI76_RS24945 are separated by 128 nucleotides
BWI76_RS24925: BWI76_RS24925 - DnaA initiator-associating protein DiaA, at 5,193,886 to 5,194,476
_RS24925
BWI76_RS24930: BWI76_RS24930 - osmotically-inducible protein OsmY, at 5,194,486 to 5,195,061
_RS24930
BWI76_RS24935: BWI76_RS24935 - hypothetical protein, at 5,195,269 to 5,196,309
_RS24935
BWI76_RS24940: BWI76_RS24940 - hypothetical protein, at 5,196,382 to 5,197,029
_RS24940
BWI76_RS24945: BWI76_RS24945 - glutamine amidotransferase, at 5,197,158 to 5,197,676
_RS24945
Position (kb) 5195
5196
5197 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5194.314 kb on - strand, within BWI76_RS24925 at 5194.493 kb on - strand at 5194.610 kb on + strand, within BWI76_RS24930 at 5194.610 kb on + strand, within BWI76_RS24930 at 5194.722 kb on - strand, within BWI76_RS24930 at 5194.745 kb on - strand, within BWI76_RS24930 at 5194.847 kb on - strand, within BWI76_RS24930 at 5194.878 kb on + strand, within BWI76_RS24930 at 5194.901 kb on - strand, within BWI76_RS24930 at 5194.953 kb on + strand, within BWI76_RS24930 at 5194.961 kb on - strand, within BWI76_RS24930 at 5195.081 kb on + strand at 5195.393 kb on - strand, within BWI76_RS24935 at 5195.516 kb on + strand, within BWI76_RS24935 at 5195.516 kb on + strand, within BWI76_RS24935 at 5195.528 kb on + strand, within BWI76_RS24935 at 5195.803 kb on + strand, within BWI76_RS24935 at 5195.811 kb on - strand, within BWI76_RS24935 at 5195.857 kb on - strand, within BWI76_RS24935 at 5195.971 kb on - strand, within BWI76_RS24935 at 5195.994 kb on + strand, within BWI76_RS24935 at 5196.154 kb on - strand, within BWI76_RS24935 at 5196.290 kb on + strand at 5196.343 kb on + strand at 5196.503 kb on + strand, within BWI76_RS24940 at 5196.869 kb on + strand, within BWI76_RS24940 at 5196.877 kb on - strand, within BWI76_RS24940 at 5196.877 kb on - strand, within BWI76_RS24940 at 5197.147 kb on - strand at 5197.174 kb on - strand at 5197.206 kb on + strand at 5197.251 kb on - strand, within BWI76_RS24945
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 5,194,314 - BWI76_RS24925 0.72 +0.4 5,194,493 - +0.9 5,194,610 + BWI76_RS24930 0.22 +0.5 5,194,610 + BWI76_RS24930 0.22 -2.7 5,194,722 - BWI76_RS24930 0.41 +0.4 5,194,745 - BWI76_RS24930 0.45 +1.7 5,194,847 - BWI76_RS24930 0.63 +0.5 5,194,878 + BWI76_RS24930 0.68 +0.5 5,194,901 - BWI76_RS24930 0.72 +0.4 5,194,953 + BWI76_RS24930 0.81 -3.4 5,194,961 - BWI76_RS24930 0.82 -0.1 5,195,081 + +0.9 5,195,393 - BWI76_RS24935 0.12 +1.1 5,195,516 + BWI76_RS24935 0.24 +1.1 5,195,516 + BWI76_RS24935 0.24 +0.2 5,195,528 + BWI76_RS24935 0.25 +1.0 5,195,803 + BWI76_RS24935 0.51 +0.9 5,195,811 - BWI76_RS24935 0.52 -1.4 5,195,857 - BWI76_RS24935 0.56 +0.4 5,195,971 - BWI76_RS24935 0.67 +0.2 5,195,994 + BWI76_RS24935 0.70 +0.6 5,196,154 - BWI76_RS24935 0.85 +1.1 5,196,290 + -1.2 5,196,343 + -0.2 5,196,503 + BWI76_RS24940 0.19 -0.6 5,196,869 + BWI76_RS24940 0.75 -3.1 5,196,877 - BWI76_RS24940 0.76 +1.0 5,196,877 - BWI76_RS24940 0.76 +0.2 5,197,147 - -0.6 5,197,174 - +0.1 5,197,206 + +0.4 5,197,251 - BWI76_RS24945 0.18 -0.4
Or see this region's nucleotide sequence