Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23320

Experiment: KLEB1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23315 and BWI76_RS23320 are separated by 23 nucleotidesBWI76_RS23320 and BWI76_RS23325 are separated by 19 nucleotidesBWI76_RS23325 and BWI76_RS23330 are separated by 94 nucleotides BWI76_RS23315: BWI76_RS23315 - acyl-[ACP]--phospholipid O-acyltransferase, at 4,862,058 to 4,864,217 _RS23315 BWI76_RS23320: BWI76_RS23320 - hypothetical protein, at 4,864,241 to 4,864,432 _RS23320 BWI76_RS23325: BWI76_RS23325 - hypothetical protein, at 4,864,452 to 4,864,745 _RS23325 BWI76_RS23330: BWI76_RS23330 - transcriptional regulator GalR, at 4,864,840 to 4,865,856 _RS23330 Position (kb) 4864 4865Strain fitness (log2 ratio) -2 -1 0 1 2at 4863.333 kb on + strand, within BWI76_RS23315at 4863.340 kb on + strand, within BWI76_RS23315at 4863.340 kb on + strand, within BWI76_RS23315at 4863.341 kb on - strand, within BWI76_RS23315at 4863.341 kb on - strand, within BWI76_RS23315at 4863.355 kb on + strand, within BWI76_RS23315at 4863.667 kb on + strand, within BWI76_RS23315at 4863.927 kb on + strand, within BWI76_RS23315at 4864.174 kb on - strandat 4864.174 kb on - strandat 4864.313 kb on - strand, within BWI76_RS23320at 4864.485 kb on + strand, within BWI76_RS23325at 4864.485 kb on + strand, within BWI76_RS23325at 4864.495 kb on + strand, within BWI76_RS23325at 4864.529 kb on + strand, within BWI76_RS23325at 4864.729 kb on + strandat 4864.737 kb on - strandat 4864.753 kb on - strandat 4865.021 kb on + strand, within BWI76_RS23330at 4865.038 kb on + strand, within BWI76_RS23330at 4865.092 kb on + strand, within BWI76_RS23330at 4865.276 kb on - strand, within BWI76_RS23330at 4865.306 kb on - strand, within BWI76_RS23330at 4865.395 kb on - strand, within BWI76_RS23330

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 10
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4,863,333 + BWI76_RS23315 0.59 +0.2
4,863,340 + BWI76_RS23315 0.59 -0.1
4,863,340 + BWI76_RS23315 0.59 +2.1
4,863,341 - BWI76_RS23315 0.59 +0.2
4,863,341 - BWI76_RS23315 0.59 +0.7
4,863,355 + BWI76_RS23315 0.60 +0.7
4,863,667 + BWI76_RS23315 0.74 +0.6
4,863,927 + BWI76_RS23315 0.87 -2.0
4,864,174 - -1.0
4,864,174 - -0.6
4,864,313 - BWI76_RS23320 0.38 +0.5
4,864,485 + BWI76_RS23325 0.11 -2.5
4,864,485 + BWI76_RS23325 0.11 -0.4
4,864,495 + BWI76_RS23325 0.15 -1.2
4,864,529 + BWI76_RS23325 0.26 +0.0
4,864,729 + +0.8
4,864,737 - +0.2
4,864,753 - +1.2
4,865,021 + BWI76_RS23330 0.18 -2.0
4,865,038 + BWI76_RS23330 0.19 +1.2
4,865,092 + BWI76_RS23330 0.25 +0.6
4,865,276 - BWI76_RS23330 0.43 -0.5
4,865,306 - BWI76_RS23330 0.46 +0.2
4,865,395 - BWI76_RS23330 0.55 -2.1

Or see this region's nucleotide sequence