Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17855 and BWI76_RS17860 are separated by 1 nucleotides BWI76_RS17860 and BWI76_RS17865 are separated by 90 nucleotides BWI76_RS17865 and BWI76_RS17870 are separated by 3 nucleotides BWI76_RS17870 and BWI76_RS17875 are separated by 162 nucleotides
BWI76_RS17855: BWI76_RS17855 - alginate lyase, at 3,712,073 to 3,714,103
_RS17855
BWI76_RS17860: BWI76_RS17860 - YoaH family protein, at 3,714,105 to 3,714,284
_RS17860
BWI76_RS17865: BWI76_RS17865 - aminodeoxychorismate synthase subunit I, at 3,714,375 to 3,715,730
_RS17865
BWI76_RS17870: BWI76_RS17870 - coenzyme A pyrophosphatase, at 3,715,734 to 3,716,312
_RS17870
BWI76_RS17875: BWI76_RS17875 - L-serine ammonia-lyase, at 3,716,475 to 3,717,839
_RS17875
Position (kb)
3714
3715
3716 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3713.394 kb on + strand, within BWI76_RS17855 at 3713.514 kb on + strand, within BWI76_RS17855 at 3713.580 kb on + strand, within BWI76_RS17855 at 3713.800 kb on + strand, within BWI76_RS17855 at 3714.075 kb on + strand at 3714.094 kb on - strand at 3714.194 kb on - strand, within BWI76_RS17860 at 3714.261 kb on - strand at 3714.368 kb on - strand at 3714.368 kb on - strand at 3714.445 kb on + strand at 3714.452 kb on + strand at 3714.618 kb on + strand, within BWI76_RS17865 at 3714.642 kb on - strand, within BWI76_RS17865 at 3714.906 kb on - strand, within BWI76_RS17865 at 3714.927 kb on + strand, within BWI76_RS17865 at 3714.965 kb on + strand, within BWI76_RS17865 at 3714.974 kb on - strand, within BWI76_RS17865 at 3714.975 kb on + strand, within BWI76_RS17865 at 3715.131 kb on - strand, within BWI76_RS17865 at 3715.175 kb on + strand, within BWI76_RS17865 at 3715.221 kb on - strand, within BWI76_RS17865 at 3715.221 kb on - strand, within BWI76_RS17865 at 3715.391 kb on - strand, within BWI76_RS17865 at 3715.940 kb on + strand, within BWI76_RS17870 at 3715.964 kb on - strand, within BWI76_RS17870 at 3716.079 kb on - strand, within BWI76_RS17870 at 3716.091 kb on - strand, within BWI76_RS17870 at 3716.219 kb on + strand, within BWI76_RS17870 at 3716.236 kb on - strand, within BWI76_RS17870 at 3716.320 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 3,713,394 + BWI76_RS17855 0.65 +1.5 3,713,514 + BWI76_RS17855 0.71 +0.1 3,713,580 + BWI76_RS17855 0.74 -0.6 3,713,800 + BWI76_RS17855 0.85 -0.5 3,714,075 + -1.2 3,714,094 - +0.8 3,714,194 - BWI76_RS17860 0.49 -2.8 3,714,261 - +1.2 3,714,368 - -3.2 3,714,368 - +0.4 3,714,445 + -0.6 3,714,452 + +0.4 3,714,618 + BWI76_RS17865 0.18 +1.5 3,714,642 - BWI76_RS17865 0.20 +0.9 3,714,906 - BWI76_RS17865 0.39 +1.1 3,714,927 + BWI76_RS17865 0.41 +1.0 3,714,965 + BWI76_RS17865 0.44 -1.2 3,714,974 - BWI76_RS17865 0.44 -0.2 3,714,975 + BWI76_RS17865 0.44 -2.1 3,715,131 - BWI76_RS17865 0.56 +0.9 3,715,175 + BWI76_RS17865 0.59 +1.9 3,715,221 - BWI76_RS17865 0.62 +0.7 3,715,221 - BWI76_RS17865 0.62 +0.0 3,715,391 - BWI76_RS17865 0.75 -1.4 3,715,940 + BWI76_RS17870 0.36 -0.2 3,715,964 - BWI76_RS17870 0.40 +1.0 3,716,079 - BWI76_RS17870 0.60 +0.5 3,716,091 - BWI76_RS17870 0.62 +1.0 3,716,219 + BWI76_RS17870 0.84 -2.2 3,716,236 - BWI76_RS17870 0.87 -0.7 3,716,320 - -0.0
Or see this region's nucleotide sequence