Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17095

Experiment: KLEB1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17085 and BWI76_RS17090 are separated by 126 nucleotidesBWI76_RS17090 and BWI76_RS17095 are separated by 81 nucleotidesBWI76_RS17095 and BWI76_RS17100 overlap by 1 nucleotidesBWI76_RS17100 and BWI76_RS17105 are separated by 224 nucleotides BWI76_RS17085: BWI76_RS17085 - hypothetical protein, at 3,545,195 to 3,546,637 _RS17085 BWI76_RS17090: BWI76_RS17090 - NLP/P60 protein, at 3,546,764 to 3,547,228 _RS17090 BWI76_RS17095: BWI76_RS17095 - vitamin B12 ABC transporter ATP-binding protein BtuD, at 3,547,310 to 3,548,059 _RS17095 BWI76_RS17100: BWI76_RS17100 - glutathione peroxidase, at 3,548,059 to 3,548,610 _RS17100 BWI76_RS17105: BWI76_RS17105 - methionine ABC transporter substrate-binding protein MetQ, at 3,548,835 to 3,549,635 _RS17105 Position (kb) 3547 3548 3549Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3546.374 kb on - strand, within BWI76_RS17085at 3546.465 kb on + strand, within BWI76_RS17085at 3546.547 kb on - strandat 3547.496 kb on + strand, within BWI76_RS17095at 3547.564 kb on + strand, within BWI76_RS17095at 3547.695 kb on + strand, within BWI76_RS17095at 3547.766 kb on - strand, within BWI76_RS17095at 3547.766 kb on - strand, within BWI76_RS17095at 3547.850 kb on + strand, within BWI76_RS17095at 3548.026 kb on + strandat 3548.387 kb on - strand, within BWI76_RS17100at 3548.402 kb on - strand, within BWI76_RS17100at 3548.654 kb on + strandat 3548.743 kb on - strandat 3548.856 kb on + strandat 3548.868 kb on + strandat 3548.882 kb on + strandat 3548.890 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 10
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3,546,374 - BWI76_RS17085 0.82 -0.2
3,546,465 + BWI76_RS17085 0.88 +0.4
3,546,547 - +0.0
3,547,496 + BWI76_RS17095 0.25 +0.2
3,547,564 + BWI76_RS17095 0.34 -3.8
3,547,695 + BWI76_RS17095 0.51 -0.8
3,547,766 - BWI76_RS17095 0.61 -1.1
3,547,766 - BWI76_RS17095 0.61 +0.0
3,547,850 + BWI76_RS17095 0.72 -0.7
3,548,026 + -1.3
3,548,387 - BWI76_RS17100 0.59 +0.7
3,548,402 - BWI76_RS17100 0.62 -0.3
3,548,654 + -2.1
3,548,743 - +0.8
3,548,856 + -1.1
3,548,868 + -0.9
3,548,882 + -0.5
3,548,890 - -2.8

Or see this region's nucleotide sequence