Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17085 and BWI76_RS17090 are separated by 126 nucleotides BWI76_RS17090 and BWI76_RS17095 are separated by 81 nucleotides BWI76_RS17095 and BWI76_RS17100 overlap by 1 nucleotides BWI76_RS17100 and BWI76_RS17105 are separated by 224 nucleotides
BWI76_RS17085: BWI76_RS17085 - hypothetical protein, at 3,545,195 to 3,546,637
_RS17085
BWI76_RS17090: BWI76_RS17090 - NLP/P60 protein, at 3,546,764 to 3,547,228
_RS17090
BWI76_RS17095: BWI76_RS17095 - vitamin B12 ABC transporter ATP-binding protein BtuD, at 3,547,310 to 3,548,059
_RS17095
BWI76_RS17100: BWI76_RS17100 - glutathione peroxidase, at 3,548,059 to 3,548,610
_RS17100
BWI76_RS17105: BWI76_RS17105 - methionine ABC transporter substrate-binding protein MetQ, at 3,548,835 to 3,549,635
_RS17105
Position (kb)
3547
3548
3549 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3546.374 kb on - strand, within BWI76_RS17085 at 3546.465 kb on + strand, within BWI76_RS17085 at 3546.547 kb on - strand at 3547.496 kb on + strand, within BWI76_RS17095 at 3547.564 kb on + strand, within BWI76_RS17095 at 3547.695 kb on + strand, within BWI76_RS17095 at 3547.766 kb on - strand, within BWI76_RS17095 at 3547.766 kb on - strand, within BWI76_RS17095 at 3547.850 kb on + strand, within BWI76_RS17095 at 3548.026 kb on + strand at 3548.387 kb on - strand, within BWI76_RS17100 at 3548.402 kb on - strand, within BWI76_RS17100 at 3548.654 kb on + strand at 3548.743 kb on - strand at 3548.856 kb on + strand at 3548.868 kb on + strand at 3548.882 kb on + strand at 3548.890 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 3,546,374 - BWI76_RS17085 0.82 -0.2 3,546,465 + BWI76_RS17085 0.88 +0.4 3,546,547 - +0.0 3,547,496 + BWI76_RS17095 0.25 +0.2 3,547,564 + BWI76_RS17095 0.34 -3.8 3,547,695 + BWI76_RS17095 0.51 -0.8 3,547,766 - BWI76_RS17095 0.61 -1.1 3,547,766 - BWI76_RS17095 0.61 +0.0 3,547,850 + BWI76_RS17095 0.72 -0.7 3,548,026 + -1.3 3,548,387 - BWI76_RS17100 0.59 +0.7 3,548,402 - BWI76_RS17100 0.62 -0.3 3,548,654 + -2.1 3,548,743 - +0.8 3,548,856 + -1.1 3,548,868 + -0.9 3,548,882 + -0.5 3,548,890 - -2.8
Or see this region's nucleotide sequence