Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15755 and BWI76_RS15760 overlap by 4 nucleotides BWI76_RS15760 and BWI76_RS15765 overlap by 17 nucleotides BWI76_RS15765 and BWI76_RS15770 overlap by 4 nucleotides BWI76_RS15770 and BWI76_RS15775 overlap by 4 nucleotides BWI76_RS15775 and BWI76_RS15780 are separated by 48 nucleotides
BWI76_RS15755: BWI76_RS15755 - hypothetical protein, at 3,282,451 to 3,282,711
_RS15755
BWI76_RS15760: BWI76_RS15760 - hypothetical protein, at 3,282,708 to 3,282,899
_RS15760
BWI76_RS15765: BWI76_RS15765 - hypothetical protein, at 3,282,883 to 3,283,599
_RS15765
BWI76_RS15770: BWI76_RS15770 - hypothetical protein, at 3,283,596 to 3,283,901
_RS15770
BWI76_RS15775: BWI76_RS15775 - replication protein, at 3,283,898 to 3,284,224
_RS15775
BWI76_RS15780: BWI76_RS15780 - hypothetical protein, at 3,284,273 to 3,285,513
_RS15780
Position (kb) 3283
3284 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3282.604 kb on - strand, within BWI76_RS15755 at 3282.604 kb on - strand, within BWI76_RS15755 at 3282.604 kb on - strand, within BWI76_RS15755 at 3282.604 kb on - strand, within BWI76_RS15755 at 3282.606 kb on - strand, within BWI76_RS15755 at 3282.654 kb on + strand, within BWI76_RS15755 at 3282.687 kb on + strand at 3282.729 kb on - strand, within BWI76_RS15760 at 3282.890 kb on + strand at 3282.928 kb on - strand at 3283.036 kb on - strand, within BWI76_RS15765 at 3283.144 kb on + strand, within BWI76_RS15765 at 3283.201 kb on - strand, within BWI76_RS15765 at 3283.292 kb on + strand, within BWI76_RS15765 at 3283.733 kb on - strand, within BWI76_RS15770 at 3283.740 kb on - strand, within BWI76_RS15770 at 3283.773 kb on - strand, within BWI76_RS15770 at 3283.804 kb on - strand, within BWI76_RS15770 at 3283.965 kb on - strand, within BWI76_RS15775
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 3,282,604 - BWI76_RS15755 0.59 -1.6 3,282,604 - BWI76_RS15755 0.59 -0.2 3,282,604 - BWI76_RS15755 0.59 -1.7 3,282,604 - BWI76_RS15755 0.59 -2.4 3,282,606 - BWI76_RS15755 0.59 -0.9 3,282,654 + BWI76_RS15755 0.78 +0.4 3,282,687 + +1.0 3,282,729 - BWI76_RS15760 0.11 -0.4 3,282,890 + -2.6 3,282,928 - -1.0 3,283,036 - BWI76_RS15765 0.21 -0.2 3,283,144 + BWI76_RS15765 0.36 -0.8 3,283,201 - BWI76_RS15765 0.44 -0.5 3,283,292 + BWI76_RS15765 0.57 -0.3 3,283,733 - BWI76_RS15770 0.45 -2.9 3,283,740 - BWI76_RS15770 0.47 -0.9 3,283,773 - BWI76_RS15770 0.58 -3.4 3,283,804 - BWI76_RS15770 0.68 -0.9 3,283,965 - BWI76_RS15775 0.20 +0.4
Or see this region's nucleotide sequence