Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14690

Experiment: KLEB1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14680 and BWI76_RS14685 overlap by 1 nucleotidesBWI76_RS14685 and BWI76_RS14690 are separated by 32 nucleotidesBWI76_RS14690 and BWI76_RS14695 are separated by 19 nucleotides BWI76_RS14680: BWI76_RS14680 - SefF, at 3,082,377 to 3,082,901 _RS14680 BWI76_RS14685: BWI76_RS14685 - fimbrial protein, at 3,082,901 to 3,083,494 _RS14685 BWI76_RS14690: BWI76_RS14690 - SefD, at 3,083,527 to 3,084,261 _RS14690 BWI76_RS14695: BWI76_RS14695 - fimbrial biogenesis outer membrane usher protein, at 3,084,281 to 3,086,782 _RS14695 Position (kb) 3083 3084 3085Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3082.639 kb on - strand, within BWI76_RS14680at 3082.708 kb on - strand, within BWI76_RS14680at 3082.828 kb on - strand, within BWI76_RS14680at 3082.871 kb on + strandat 3082.924 kb on + strandat 3083.280 kb on + strand, within BWI76_RS14685at 3083.512 kb on - strandat 3083.712 kb on + strand, within BWI76_RS14690at 3083.894 kb on - strand, within BWI76_RS14690at 3084.099 kb on + strand, within BWI76_RS14690at 3084.107 kb on - strand, within BWI76_RS14690at 3084.107 kb on - strand, within BWI76_RS14690at 3084.180 kb on - strand, within BWI76_RS14690at 3084.180 kb on - strand, within BWI76_RS14690at 3084.190 kb on - strandat 3084.335 kb on - strandat 3084.369 kb on + strandat 3084.462 kb on - strandat 3084.549 kb on - strand, within BWI76_RS14695at 3084.923 kb on - strand, within BWI76_RS14695at 3084.923 kb on - strand, within BWI76_RS14695at 3085.164 kb on - strandat 3085.171 kb on + strand, within BWI76_RS14695at 3085.237 kb on - strand, within BWI76_RS14695

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 10
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3,082,639 - BWI76_RS14680 0.50 -0.1
3,082,708 - BWI76_RS14680 0.63 +1.0
3,082,828 - BWI76_RS14680 0.86 +2.5
3,082,871 + -1.5
3,082,924 + +1.9
3,083,280 + BWI76_RS14685 0.64 -3.3
3,083,512 - -0.1
3,083,712 + BWI76_RS14690 0.25 -0.4
3,083,894 - BWI76_RS14690 0.50 +1.5
3,084,099 + BWI76_RS14690 0.78 +2.9
3,084,107 - BWI76_RS14690 0.79 +1.1
3,084,107 - BWI76_RS14690 0.79 -0.1
3,084,180 - BWI76_RS14690 0.89 +1.4
3,084,180 - BWI76_RS14690 0.89 +0.5
3,084,190 - +0.8
3,084,335 - +0.8
3,084,369 + -0.2
3,084,462 - -0.1
3,084,549 - BWI76_RS14695 0.11 +1.3
3,084,923 - BWI76_RS14695 0.26 +0.3
3,084,923 - BWI76_RS14695 0.26 -1.5
3,085,164 - -0.6
3,085,171 + BWI76_RS14695 0.36 -3.2
3,085,237 - BWI76_RS14695 0.38 -1.3

Or see this region's nucleotide sequence