Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14680 and BWI76_RS14685 overlap by 1 nucleotides BWI76_RS14685 and BWI76_RS14690 are separated by 32 nucleotides BWI76_RS14690 and BWI76_RS14695 are separated by 19 nucleotides
BWI76_RS14680: BWI76_RS14680 - SefF, at 3,082,377 to 3,082,901
_RS14680
BWI76_RS14685: BWI76_RS14685 - fimbrial protein, at 3,082,901 to 3,083,494
_RS14685
BWI76_RS14690: BWI76_RS14690 - SefD, at 3,083,527 to 3,084,261
_RS14690
BWI76_RS14695: BWI76_RS14695 - fimbrial biogenesis outer membrane usher protein, at 3,084,281 to 3,086,782
_RS14695
Position (kb)
3083
3084
3085 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3082.639 kb on - strand, within BWI76_RS14680 at 3082.708 kb on - strand, within BWI76_RS14680 at 3082.828 kb on - strand, within BWI76_RS14680 at 3082.871 kb on + strand at 3082.924 kb on + strand at 3083.280 kb on + strand, within BWI76_RS14685 at 3083.512 kb on - strand at 3083.712 kb on + strand, within BWI76_RS14690 at 3083.894 kb on - strand, within BWI76_RS14690 at 3084.099 kb on + strand, within BWI76_RS14690 at 3084.107 kb on - strand, within BWI76_RS14690 at 3084.107 kb on - strand, within BWI76_RS14690 at 3084.180 kb on - strand, within BWI76_RS14690 at 3084.180 kb on - strand, within BWI76_RS14690 at 3084.190 kb on - strand at 3084.335 kb on - strand at 3084.369 kb on + strand at 3084.462 kb on - strand at 3084.549 kb on - strand, within BWI76_RS14695 at 3084.923 kb on - strand, within BWI76_RS14695 at 3084.923 kb on - strand, within BWI76_RS14695 at 3085.164 kb on - strand at 3085.171 kb on + strand, within BWI76_RS14695 at 3085.237 kb on - strand, within BWI76_RS14695
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 3,082,639 - BWI76_RS14680 0.50 -0.1 3,082,708 - BWI76_RS14680 0.63 +1.0 3,082,828 - BWI76_RS14680 0.86 +2.5 3,082,871 + -1.5 3,082,924 + +1.9 3,083,280 + BWI76_RS14685 0.64 -3.3 3,083,512 - -0.1 3,083,712 + BWI76_RS14690 0.25 -0.4 3,083,894 - BWI76_RS14690 0.50 +1.5 3,084,099 + BWI76_RS14690 0.78 +2.9 3,084,107 - BWI76_RS14690 0.79 +1.1 3,084,107 - BWI76_RS14690 0.79 -0.1 3,084,180 - BWI76_RS14690 0.89 +1.4 3,084,180 - BWI76_RS14690 0.89 +0.5 3,084,190 - +0.8 3,084,335 - +0.8 3,084,369 + -0.2 3,084,462 - -0.1 3,084,549 - BWI76_RS14695 0.11 +1.3 3,084,923 - BWI76_RS14695 0.26 +0.3 3,084,923 - BWI76_RS14695 0.26 -1.5 3,085,164 - -0.6 3,085,171 + BWI76_RS14695 0.36 -3.2 3,085,237 - BWI76_RS14695 0.38 -1.3
Or see this region's nucleotide sequence