Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13525

Experiment: KLEB1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13520 and BWI76_RS13525 are separated by 298 nucleotidesBWI76_RS13525 and BWI76_RS13530 overlap by 4 nucleotides BWI76_RS13520: BWI76_RS13520 - hypothetical protein, at 2,819,208 to 2,819,747 _RS13520 BWI76_RS13525: BWI76_RS13525 - putative luciferase-like monooxygenase, at 2,820,046 to 2,821,047 _RS13525 BWI76_RS13530: BWI76_RS13530 - amidohydrolase family protein, at 2,821,044 to 2,822,165 _RS13530 Position (kb) 2820 2821 2822Strain fitness (log2 ratio) -2 -1 0 1 2at 2819.764 kb on + strandat 2819.772 kb on - strandat 2819.993 kb on + strandat 2820.358 kb on - strand, within BWI76_RS13525at 2820.671 kb on + strand, within BWI76_RS13525at 2820.911 kb on - strand, within BWI76_RS13525at 2820.934 kb on + strand, within BWI76_RS13525at 2820.990 kb on + strandat 2820.998 kb on - strandat 2821.427 kb on - strand, within BWI76_RS13530at 2821.601 kb on - strand, within BWI76_RS13530at 2821.712 kb on - strand, within BWI76_RS13530at 2821.732 kb on - strand, within BWI76_RS13530at 2821.811 kb on - strand, within BWI76_RS13530at 2821.907 kb on + strand, within BWI76_RS13530at 2821.907 kb on + strand, within BWI76_RS13530at 2821.907 kb on + strand, within BWI76_RS13530at 2821.954 kb on + strand, within BWI76_RS13530at 2821.982 kb on + strand, within BWI76_RS13530at 2822.029 kb on - strand, within BWI76_RS13530

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 10
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2,819,764 + +0.4
2,819,772 - -0.1
2,819,993 + -1.2
2,820,358 - BWI76_RS13525 0.31 -2.0
2,820,671 + BWI76_RS13525 0.62 -0.7
2,820,911 - BWI76_RS13525 0.86 +1.6
2,820,934 + BWI76_RS13525 0.89 +2.3
2,820,990 + -2.2
2,820,998 - +2.2
2,821,427 - BWI76_RS13530 0.34 +1.5
2,821,601 - BWI76_RS13530 0.50 +0.6
2,821,712 - BWI76_RS13530 0.60 -0.6
2,821,732 - BWI76_RS13530 0.61 -0.1
2,821,811 - BWI76_RS13530 0.68 -0.7
2,821,907 + BWI76_RS13530 0.77 +2.1
2,821,907 + BWI76_RS13530 0.77 -0.7
2,821,907 + BWI76_RS13530 0.77 +0.7
2,821,954 + BWI76_RS13530 0.81 +1.2
2,821,982 + BWI76_RS13530 0.84 +0.9
2,822,029 - BWI76_RS13530 0.88 +0.3

Or see this region's nucleotide sequence