Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09550 and BWI76_RS09555 are separated by 154 nucleotides BWI76_RS09555 and BWI76_RS09560 are separated by 151 nucleotides BWI76_RS09560 and BWI76_RS09565 are separated by 39 nucleotides
BWI76_RS09550: BWI76_RS09550 - low-specificity L-threonine aldolase, at 1,997,268 to 1,998,269
_RS09550
BWI76_RS09555: BWI76_RS09555 - pyruvate oxidase, at 1,998,424 to 2,000,142
_RS09555
BWI76_RS09560: BWI76_RS09560 - DoxX family membrane protein, at 2,000,294 to 2,000,728
_RS09560
BWI76_RS09565: BWI76_RS09565 - hybrid-cluster NAD(P)-dependent oxidoreductase, at 2,000,768 to 2,001,736
_RS09565
Position (kb)
1998
1999
2000
2001 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1997.556 kb on - strand, within BWI76_RS09550 at 1997.744 kb on - strand, within BWI76_RS09550 at 1997.750 kb on - strand, within BWI76_RS09550 at 1998.198 kb on - strand at 1998.235 kb on + strand at 1998.310 kb on - strand at 1998.320 kb on + strand at 1998.492 kb on + strand at 1998.919 kb on - strand, within BWI76_RS09555 at 1999.118 kb on - strand, within BWI76_RS09555 at 1999.411 kb on - strand, within BWI76_RS09555 at 1999.717 kb on + strand, within BWI76_RS09555 at 2000.108 kb on - strand at 2000.178 kb on + strand at 2000.441 kb on - strand, within BWI76_RS09560 at 2000.602 kb on - strand, within BWI76_RS09560 at 2000.916 kb on - strand, within BWI76_RS09565 at 2000.965 kb on + strand, within BWI76_RS09565
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 1,997,556 - BWI76_RS09550 0.29 -0.6 1,997,744 - BWI76_RS09550 0.48 -1.0 1,997,750 - BWI76_RS09550 0.48 +0.8 1,998,198 - -0.7 1,998,235 + +0.4 1,998,310 - -2.0 1,998,320 + +0.9 1,998,492 + -3.5 1,998,919 - BWI76_RS09555 0.29 +0.5 1,999,118 - BWI76_RS09555 0.40 +1.0 1,999,411 - BWI76_RS09555 0.57 +1.1 1,999,717 + BWI76_RS09555 0.75 +0.8 2,000,108 - +0.3 2,000,178 + -2.3 2,000,441 - BWI76_RS09560 0.34 -0.6 2,000,602 - BWI76_RS09560 0.71 -2.8 2,000,916 - BWI76_RS09565 0.15 -0.3 2,000,965 + BWI76_RS09565 0.20 -0.4
Or see this region's nucleotide sequence