Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07615 and BWI76_RS07620 are separated by 10 nucleotides BWI76_RS07620 and BWI76_RS07625 overlap by 84 nucleotides BWI76_RS07625 and BWI76_RS07630 overlap by 4 nucleotides
BWI76_RS07615: BWI76_RS07615 - betaine-aldehyde dehydrogenase, at 1,613,138 to 1,614,610
_RS07615
BWI76_RS07620: BWI76_RS07620 - transcriptional regulator BetI, at 1,614,621 to 1,615,214
_RS07620
BWI76_RS07625: BWI76_RS07625 - hypothetical protein, at 1,615,131 to 1,615,346
_RS07625
BWI76_RS07630: BWI76_RS07630 - high-affinity choline transporter BetT, at 1,615,343 to 1,617,376
_RS07630
Position (kb)
1614
1615
1616 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1613.764 kb on - strand, within BWI76_RS07615 at 1613.769 kb on - strand, within BWI76_RS07615 at 1613.777 kb on - strand, within BWI76_RS07615 at 1614.049 kb on + strand, within BWI76_RS07615 at 1614.090 kb on - strand, within BWI76_RS07615 at 1614.458 kb on + strand, within BWI76_RS07615 at 1614.501 kb on + strand at 1614.501 kb on + strand at 1614.605 kb on + strand at 1614.702 kb on + strand, within BWI76_RS07620 at 1614.818 kb on + strand, within BWI76_RS07620 at 1615.001 kb on - strand, within BWI76_RS07620 at 1615.039 kb on + strand, within BWI76_RS07620 at 1615.467 kb on + strand at 1615.478 kb on + strand at 1615.552 kb on + strand, within BWI76_RS07630 at 1615.590 kb on + strand at 1615.730 kb on - strand, within BWI76_RS07630 at 1616.014 kb on + strand at 1616.151 kb on + strand, within BWI76_RS07630 at 1616.170 kb on + strand, within BWI76_RS07630 at 1616.186 kb on - strand, within BWI76_RS07630
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 1,613,764 - BWI76_RS07615 0.42 +0.3 1,613,769 - BWI76_RS07615 0.43 +3.3 1,613,777 - BWI76_RS07615 0.43 -0.5 1,614,049 + BWI76_RS07615 0.62 -1.2 1,614,090 - BWI76_RS07615 0.65 -1.2 1,614,458 + BWI76_RS07615 0.90 -1.4 1,614,501 + -1.4 1,614,501 + +0.3 1,614,605 + -1.7 1,614,702 + BWI76_RS07620 0.14 -0.1 1,614,818 + BWI76_RS07620 0.33 -0.7 1,615,001 - BWI76_RS07620 0.64 -0.3 1,615,039 + BWI76_RS07620 0.70 -4.0 1,615,467 + +2.2 1,615,478 + +0.7 1,615,552 + BWI76_RS07630 0.10 -4.7 1,615,590 + +0.5 1,615,730 - BWI76_RS07630 0.19 +0.2 1,616,014 + -0.5 1,616,151 + BWI76_RS07630 0.40 -0.3 1,616,170 + BWI76_RS07630 0.41 -3.2 1,616,186 - BWI76_RS07630 0.41 +0.1
Or see this region's nucleotide sequence