Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05420 and BWI76_RS05425 are separated by 94 nucleotides BWI76_RS05425 and BWI76_RS05430 are separated by 84 nucleotides BWI76_RS05430 and BWI76_RS05435 are separated by 57 nucleotides
BWI76_RS05420: BWI76_RS05420 - MFS transporter, at 1,138,012 to 1,139,202
_RS05420
BWI76_RS05425: BWI76_RS05425 - EEP domain-containing protein, at 1,139,297 to 1,140,103
_RS05425
BWI76_RS05430: BWI76_RS05430 - UbiE/COQ5 family methyltransferase, at 1,140,188 to 1,140,958
_RS05430
BWI76_RS05435: BWI76_RS05435 - murein transglycosylase D, at 1,141,016 to 1,142,383
_RS05435
Position (kb)
1140
1141 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1139.205 kb on - strand at 1139.242 kb on + strand at 1139.367 kb on - strand at 1139.456 kb on + strand, within BWI76_RS05425 at 1139.801 kb on - strand, within BWI76_RS05425 at 1139.890 kb on - strand, within BWI76_RS05425 at 1140.133 kb on + strand at 1140.211 kb on - strand at 1140.243 kb on + strand at 1140.251 kb on - strand at 1140.256 kb on - strand at 1140.481 kb on + strand, within BWI76_RS05430 at 1140.540 kb on + strand, within BWI76_RS05430 at 1140.752 kb on - strand, within BWI76_RS05430 at 1141.076 kb on - strand at 1141.147 kb on - strand at 1141.154 kb on + strand, within BWI76_RS05435 at 1141.358 kb on + strand, within BWI76_RS05435 at 1141.378 kb on - strand, within BWI76_RS05435 at 1141.415 kb on + strand, within BWI76_RS05435 at 1141.503 kb on - strand, within BWI76_RS05435 at 1141.592 kb on + strand, within BWI76_RS05435 at 1141.632 kb on - strand, within BWI76_RS05435 at 1141.795 kb on - strand, within BWI76_RS05435
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 1,139,205 - +3.3 1,139,242 + -1.7 1,139,367 - -3.9 1,139,456 + BWI76_RS05425 0.20 -0.1 1,139,801 - BWI76_RS05425 0.62 -0.2 1,139,890 - BWI76_RS05425 0.73 -1.1 1,140,133 + +0.1 1,140,211 - -4.8 1,140,243 + -1.4 1,140,251 - +0.2 1,140,256 - +0.1 1,140,481 + BWI76_RS05430 0.38 -0.1 1,140,540 + BWI76_RS05430 0.46 -0.8 1,140,752 - BWI76_RS05430 0.73 -4.7 1,141,076 - -1.1 1,141,147 - -1.6 1,141,154 + BWI76_RS05435 0.10 -4.7 1,141,358 + BWI76_RS05435 0.25 -0.2 1,141,378 - BWI76_RS05435 0.26 -0.9 1,141,415 + BWI76_RS05435 0.29 -1.1 1,141,503 - BWI76_RS05435 0.36 -0.5 1,141,592 + BWI76_RS05435 0.42 +0.9 1,141,632 - BWI76_RS05435 0.45 +1.0 1,141,795 - BWI76_RS05435 0.57 -1.4
Or see this region's nucleotide sequence