Experiment: KLEB1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04115 and BWI76_RS04120 overlap by 4 nucleotides BWI76_RS04120 and BWI76_RS04125 are separated by 145 nucleotides BWI76_RS04125 and BWI76_RS04130 are separated by 414 nucleotides
BWI76_RS04115: BWI76_RS04115 - patatin family protein, at 845,881 to 846,954
_RS04115
BWI76_RS04120: BWI76_RS04120 - metal-dependent hydrolase, at 846,951 to 847,730
_RS04120
BWI76_RS04125: BWI76_RS04125 - NupC/NupG family nucleoside CNT transporter, at 847,876 to 849,153
_RS04125
BWI76_RS04130: BWI76_RS04130 - 2-deoxyribose-5-phosphate aldolase, at 849,568 to 850,347
_RS04130
Position (kb) 847
848
849
850 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 847.035 kb on + strand, within BWI76_RS04120 at 847.188 kb on - strand, within BWI76_RS04120 at 847.211 kb on + strand, within BWI76_RS04120 at 847.559 kb on + strand, within BWI76_RS04120 at 847.575 kb on - strand, within BWI76_RS04120 at 847.615 kb on + strand, within BWI76_RS04120 at 847.615 kb on + strand at 847.840 kb on + strand at 847.840 kb on + strand at 847.840 kb on + strand at 847.848 kb on - strand at 848.038 kb on - strand, within BWI76_RS04125 at 848.161 kb on + strand, within BWI76_RS04125 at 848.199 kb on - strand, within BWI76_RS04125 at 848.307 kb on - strand, within BWI76_RS04125 at 848.307 kb on - strand, within BWI76_RS04125 at 848.379 kb on + strand, within BWI76_RS04125 at 848.411 kb on - strand, within BWI76_RS04125 at 848.413 kb on + strand, within BWI76_RS04125 at 848.413 kb on + strand, within BWI76_RS04125 at 848.442 kb on + strand, within BWI76_RS04125 at 848.525 kb on - strand, within BWI76_RS04125 at 848.553 kb on + strand, within BWI76_RS04125 at 848.874 kb on + strand, within BWI76_RS04125 at 848.956 kb on - strand, within BWI76_RS04125 at 848.958 kb on + strand, within BWI76_RS04125 at 848.975 kb on + strand, within BWI76_RS04125 at 849.148 kb on + strand at 849.483 kb on - strand at 849.607 kb on - strand at 849.727 kb on - strand, within BWI76_RS04130 at 849.761 kb on + strand, within BWI76_RS04130 at 849.776 kb on + strand, within BWI76_RS04130 at 849.828 kb on + strand, within BWI76_RS04130 at 849.870 kb on - strand, within BWI76_RS04130 at 849.928 kb on - strand, within BWI76_RS04130 at 849.933 kb on - strand, within BWI76_RS04130
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 10 remove 847,035 + BWI76_RS04120 0.11 -0.1 847,188 - BWI76_RS04120 0.30 -1.6 847,211 + BWI76_RS04120 0.33 -0.1 847,559 + BWI76_RS04120 0.78 -1.9 847,575 - BWI76_RS04120 0.80 -0.2 847,615 + BWI76_RS04120 0.85 -0.3 847,615 + -0.8 847,840 + -2.1 847,840 + -1.0 847,840 + -1.2 847,848 - -4.4 848,038 - BWI76_RS04125 0.13 +0.8 848,161 + BWI76_RS04125 0.22 +1.6 848,199 - BWI76_RS04125 0.25 +0.1 848,307 - BWI76_RS04125 0.34 -2.9 848,307 - BWI76_RS04125 0.34 -0.3 848,379 + BWI76_RS04125 0.39 +0.1 848,411 - BWI76_RS04125 0.42 -2.9 848,413 + BWI76_RS04125 0.42 -0.2 848,413 + BWI76_RS04125 0.42 -0.6 848,442 + BWI76_RS04125 0.44 +0.5 848,525 - BWI76_RS04125 0.51 -5.1 848,553 + BWI76_RS04125 0.53 -1.2 848,874 + BWI76_RS04125 0.78 -0.6 848,956 - BWI76_RS04125 0.85 +0.1 848,958 + BWI76_RS04125 0.85 +0.1 848,975 + BWI76_RS04125 0.86 -1.1 849,148 + -0.8 849,483 - -2.8 849,607 - +0.1 849,727 - BWI76_RS04130 0.20 -1.0 849,761 + BWI76_RS04130 0.25 +1.5 849,776 + BWI76_RS04130 0.27 +0.1 849,828 + BWI76_RS04130 0.33 -1.1 849,870 - BWI76_RS04130 0.39 -0.7 849,928 - BWI76_RS04130 0.46 +1.4 849,933 - BWI76_RS04130 0.47 +0.8
Or see this region's nucleotide sequence