Experiment: KLEB20 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22100 and BWI76_RS22105 are separated by 143 nucleotides BWI76_RS22105 and BWI76_RS22110 overlap by 22 nucleotides BWI76_RS22110 and BWI76_RS22115 are separated by 57 nucleotides
BWI76_RS22100: BWI76_RS22100 - multidrug effux MFS transporter subunit EmrB, at 4,613,002 to 4,614,540
_RS22100
BWI76_RS22105: BWI76_RS22105 - 2-isopropylmalate synthase, at 4,614,684 to 4,616,360
_RS22105
BWI76_RS22110: BWI76_RS22110 - putative helix-turn-helix AraC-type transcriptional regulator, at 4,616,339 to 4,617,106
_RS22110
BWI76_RS22115: BWI76_RS22115 - S-ribosylhomocysteine lyase, at 4,617,164 to 4,617,679
_RS22115
Position (kb) 4614
4615
4616
4617 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4613.901 kb on - strand, within BWI76_RS22100 at 4614.041 kb on + strand, within BWI76_RS22100 at 4614.109 kb on + strand, within BWI76_RS22100 at 4614.287 kb on + strand, within BWI76_RS22100 at 4614.608 kb on + strand at 4614.689 kb on + strand at 4615.021 kb on - strand, within BWI76_RS22105 at 4615.343 kb on + strand, within BWI76_RS22105 at 4615.344 kb on - strand, within BWI76_RS22105 at 4615.344 kb on - strand, within BWI76_RS22105 at 4615.351 kb on - strand, within BWI76_RS22105 at 4615.351 kb on - strand, within BWI76_RS22105 at 4615.753 kb on + strand, within BWI76_RS22105 at 4615.800 kb on + strand, within BWI76_RS22105 at 4616.134 kb on + strand, within BWI76_RS22105 at 4616.446 kb on + strand, within BWI76_RS22110 at 4616.842 kb on + strand, within BWI76_RS22110 at 4616.860 kb on + strand, within BWI76_RS22110 at 4616.887 kb on + strand, within BWI76_RS22110 at 4616.887 kb on + strand, within BWI76_RS22110 at 4616.887 kb on + strand, within BWI76_RS22110 at 4616.895 kb on - strand, within BWI76_RS22110 at 4616.895 kb on - strand, within BWI76_RS22110 at 4616.969 kb on - strand, within BWI76_RS22110 at 4617.301 kb on + strand, within BWI76_RS22115
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1 remove 4,613,901 - BWI76_RS22100 0.58 -0.4 4,614,041 + BWI76_RS22100 0.68 +0.2 4,614,109 + BWI76_RS22100 0.72 +0.9 4,614,287 + BWI76_RS22100 0.83 -0.3 4,614,608 + -0.6 4,614,689 + +0.5 4,615,021 - BWI76_RS22105 0.20 -1.6 4,615,343 + BWI76_RS22105 0.39 -1.0 4,615,344 - BWI76_RS22105 0.39 -0.9 4,615,344 - BWI76_RS22105 0.39 -0.6 4,615,351 - BWI76_RS22105 0.40 -0.3 4,615,351 - BWI76_RS22105 0.40 -4.2 4,615,753 + BWI76_RS22105 0.64 -1.1 4,615,800 + BWI76_RS22105 0.67 -2.0 4,616,134 + BWI76_RS22105 0.86 +0.5 4,616,446 + BWI76_RS22110 0.14 +0.9 4,616,842 + BWI76_RS22110 0.65 +1.4 4,616,860 + BWI76_RS22110 0.68 -0.2 4,616,887 + BWI76_RS22110 0.71 +0.5 4,616,887 + BWI76_RS22110 0.71 -0.6 4,616,887 + BWI76_RS22110 0.71 -0.7 4,616,895 - BWI76_RS22110 0.72 -2.1 4,616,895 - BWI76_RS22110 0.72 +0.3 4,616,969 - BWI76_RS22110 0.82 +0.5 4,617,301 + BWI76_RS22115 0.27 -0.7
Or see this region's nucleotide sequence