Experiment: KLEB20 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17400 and BWI76_RS17405 are separated by 150 nucleotides BWI76_RS17405 and BWI76_RS17410 are separated by 265 nucleotides BWI76_RS17410 and BWI76_RS17415 are separated by 89 nucleotides BWI76_RS17415 and BWI76_RS17420 are separated by 39 nucleotides
BWI76_RS17400: BWI76_RS17400 - transporter, at 3,610,065 to 3,610,745
_RS17400
BWI76_RS17405: BWI76_RS17405 - cation transport regulator, at 3,610,896 to 3,611,126
_RS17405
BWI76_RS17410: BWI76_RS17410 - sodium-potassium/proton antiporter ChaA, at 3,611,392 to 3,612,492
_RS17410
BWI76_RS17415: BWI76_RS17415 - 3-deoxy-8-phosphooctulonate synthase, at 3,612,582 to 3,613,436
_RS17415
BWI76_RS17420: BWI76_RS17420 - hypothetical protein, at 3,613,476 to 3,614,288
_RS17420
Position (kb)
3611
3612
3613 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3610.493 kb on - strand, within BWI76_RS17400 at 3611.016 kb on - strand, within BWI76_RS17405 at 3611.016 kb on - strand, within BWI76_RS17405 at 3611.082 kb on + strand at 3611.092 kb on + strand, within BWI76_RS17405 at 3611.427 kb on + strand at 3611.437 kb on + strand at 3611.539 kb on + strand, within BWI76_RS17410 at 3611.573 kb on + strand, within BWI76_RS17410 at 3611.807 kb on + strand, within BWI76_RS17410 at 3611.807 kb on + strand, within BWI76_RS17410 at 3611.807 kb on + strand, within BWI76_RS17410 at 3611.882 kb on - strand, within BWI76_RS17410
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1 remove 3,610,493 - BWI76_RS17400 0.63 -1.4 3,611,016 - BWI76_RS17405 0.52 +0.3 3,611,016 - BWI76_RS17405 0.52 -0.7 3,611,082 + +1.7 3,611,092 + BWI76_RS17405 0.85 -2.6 3,611,427 + +0.6 3,611,437 + -0.4 3,611,539 + BWI76_RS17410 0.13 +4.0 3,611,573 + BWI76_RS17410 0.16 -0.7 3,611,807 + BWI76_RS17410 0.38 +0.6 3,611,807 + BWI76_RS17410 0.38 -0.1 3,611,807 + BWI76_RS17410 0.38 +0.3 3,611,882 - BWI76_RS17410 0.45 +0.1
Or see this region's nucleotide sequence