Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13020

Experiment: KLEB20 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13015 and BWI76_RS13020 are separated by 96 nucleotidesBWI76_RS13020 and BWI76_RS13025 are separated by 137 nucleotidesBWI76_RS13025 and BWI76_RS13030 are separated by 214 nucleotides BWI76_RS13015: BWI76_RS13015 - LysR family transcriptional regulator, at 2,715,824 to 2,716,735 _RS13015 BWI76_RS13020: BWI76_RS13020 - NmrA family protein, at 2,716,832 to 2,717,821 _RS13020 BWI76_RS13025: BWI76_RS13025 - histidine phosphatase family protein, at 2,717,959 to 2,718,579 _RS13025 BWI76_RS13030: BWI76_RS13030 - site-specific recombinase, phage integrase family protein, at 2,718,794 to 2,719,396 _RS13030 Position (kb) 2716 2717 2718Strain fitness (log2 ratio) -2 -1 0 1at 2715.900 kb on + strandat 2715.905 kb on + strandat 2715.908 kb on - strandat 2715.910 kb on + strandat 2715.918 kb on - strand, within BWI76_RS13015at 2715.974 kb on - strand, within BWI76_RS13015at 2715.979 kb on - strand, within BWI76_RS13015at 2716.020 kb on - strand, within BWI76_RS13015at 2716.046 kb on - strand, within BWI76_RS13015at 2716.054 kb on + strand, within BWI76_RS13015at 2716.238 kb on - strand, within BWI76_RS13015at 2716.688 kb on - strandat 2716.728 kb on - strandat 2717.120 kb on + strand, within BWI76_RS13020at 2717.144 kb on - strand, within BWI76_RS13020at 2717.359 kb on + strand, within BWI76_RS13020at 2717.457 kb on - strand, within BWI76_RS13020at 2717.654 kb on + strand, within BWI76_RS13020at 2717.774 kb on + strandat 2718.049 kb on + strand, within BWI76_RS13025at 2718.294 kb on + strand, within BWI76_RS13025at 2718.419 kb on + strand, within BWI76_RS13025at 2718.503 kb on + strand, within BWI76_RS13025at 2718.633 kb on + strandat 2718.776 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1
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2,715,900 + -0.4
2,715,905 + -1.5
2,715,908 - +0.2
2,715,910 + -0.7
2,715,918 - BWI76_RS13015 0.10 -0.0
2,715,974 - BWI76_RS13015 0.16 -1.9
2,715,979 - BWI76_RS13015 0.17 +0.0
2,716,020 - BWI76_RS13015 0.21 +0.1
2,716,046 - BWI76_RS13015 0.24 -1.1
2,716,054 + BWI76_RS13015 0.25 -1.1
2,716,238 - BWI76_RS13015 0.45 -0.5
2,716,688 - -0.2
2,716,728 - +0.0
2,717,120 + BWI76_RS13020 0.29 -1.4
2,717,144 - BWI76_RS13020 0.32 +0.7
2,717,359 + BWI76_RS13020 0.53 -0.3
2,717,457 - BWI76_RS13020 0.63 +0.2
2,717,654 + BWI76_RS13020 0.83 -0.8
2,717,774 + +0.6
2,718,049 + BWI76_RS13025 0.14 -0.6
2,718,294 + BWI76_RS13025 0.54 -1.1
2,718,419 + BWI76_RS13025 0.74 -2.1
2,718,503 + BWI76_RS13025 0.88 +0.4
2,718,633 + -0.5
2,718,776 + +1.7

Or see this region's nucleotide sequence