Experiment: KLEB20 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12825 and BWI76_RS12830 are separated by 4 nucleotides BWI76_RS12830 and BWI76_RS12835 are separated by 23 nucleotides BWI76_RS12835 and BWI76_RS12840 are separated by 27 nucleotides
BWI76_RS12825: BWI76_RS12825 - hypothetical protein, at 2,672,888 to 2,673,619
_RS12825
BWI76_RS12830: BWI76_RS12830 - flagellar motor switch protein FliY, at 2,673,624 to 2,674,454
_RS12830
BWI76_RS12835: BWI76_RS12835 - restriction endonuclease subunit S, at 2,674,478 to 2,675,350
_RS12835
BWI76_RS12840: BWI76_RS12840 - amino acid ABC transporter permease/ATP-binding protein, at 2,675,378 to 2,676,898
_RS12840
Position (kb)
2674
2675
2676 Strain fitness (log2 ratio)
-2
-1
0
1 at 2674.325 kb on + strand, within BWI76_RS12830 at 2674.345 kb on - strand, within BWI76_RS12830 at 2674.382 kb on - strand at 2674.421 kb on + strand at 2674.667 kb on - strand, within BWI76_RS12835 at 2674.678 kb on - strand, within BWI76_RS12835 at 2674.855 kb on - strand, within BWI76_RS12835 at 2674.992 kb on - strand, within BWI76_RS12835 at 2675.088 kb on - strand, within BWI76_RS12835 at 2675.189 kb on + strand, within BWI76_RS12835 at 2675.572 kb on - strand, within BWI76_RS12840 at 2675.688 kb on + strand, within BWI76_RS12840 at 2675.688 kb on + strand, within BWI76_RS12840 at 2675.816 kb on + strand, within BWI76_RS12840 at 2675.977 kb on + strand, within BWI76_RS12840 at 2675.977 kb on + strand, within BWI76_RS12840 at 2676.009 kb on + strand, within BWI76_RS12840 at 2676.312 kb on + strand, within BWI76_RS12840 at 2676.320 kb on - strand, within BWI76_RS12840
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1 remove 2,674,325 + BWI76_RS12830 0.84 -1.0 2,674,345 - BWI76_RS12830 0.87 +1.7 2,674,382 - +0.4 2,674,421 + +0.4 2,674,667 - BWI76_RS12835 0.22 -1.1 2,674,678 - BWI76_RS12835 0.23 -0.8 2,674,855 - BWI76_RS12835 0.43 -1.7 2,674,992 - BWI76_RS12835 0.59 -0.6 2,675,088 - BWI76_RS12835 0.70 -0.5 2,675,189 + BWI76_RS12835 0.81 -0.8 2,675,572 - BWI76_RS12840 0.13 +0.2 2,675,688 + BWI76_RS12840 0.20 -2.0 2,675,688 + BWI76_RS12840 0.20 +1.1 2,675,816 + BWI76_RS12840 0.29 +0.6 2,675,977 + BWI76_RS12840 0.39 -1.5 2,675,977 + BWI76_RS12840 0.39 -0.9 2,676,009 + BWI76_RS12840 0.41 -0.6 2,676,312 + BWI76_RS12840 0.61 +0.7 2,676,320 - BWI76_RS12840 0.62 +0.4
Or see this region's nucleotide sequence