Experiment: KLEB20 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00170 and BWI76_RS00175 are separated by 345 nucleotides BWI76_RS00175 and BWI76_RS00180 are separated by 417 nucleotides BWI76_RS00180 and BWI76_RS00185 are separated by 20 nucleotides
BWI76_RS00170: BWI76_RS00170 - bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase, at 34,140 to 35,510
_RS00170
BWI76_RS00175: BWI76_RS00175 - pectate disaccharide-lyase, at 35,856 to 38,027
_RS00175
BWI76_RS00180: BWI76_RS00180 - F0F1 ATP synthase subunit epsilon, at 38,445 to 38,864
_RS00180
BWI76_RS00185: BWI76_RS00185 - ATP synthase subunit beta, at 38,885 to 40,267
_RS00185
Position (kb) 35
36
37
38
39 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 35.618 kb on - strand at 35.651 kb on + strand at 35.651 kb on + strand at 35.871 kb on + strand at 35.926 kb on - strand at 35.958 kb on - strand at 36.079 kb on - strand, within BWI76_RS00175 at 36.181 kb on - strand, within BWI76_RS00175 at 36.313 kb on - strand, within BWI76_RS00175 at 36.853 kb on - strand, within BWI76_RS00175 at 36.853 kb on - strand, within BWI76_RS00175 at 36.853 kb on - strand, within BWI76_RS00175 at 36.853 kb on - strand, within BWI76_RS00175 at 36.853 kb on - strand, within BWI76_RS00175 at 36.964 kb on + strand, within BWI76_RS00175 at 36.974 kb on - strand at 36.974 kb on - strand, within BWI76_RS00175 at 36.977 kb on - strand, within BWI76_RS00175 at 37.166 kb on + strand, within BWI76_RS00175 at 37.282 kb on + strand, within BWI76_RS00175 at 37.301 kb on + strand, within BWI76_RS00175 at 37.368 kb on - strand, within BWI76_RS00175 at 37.527 kb on - strand, within BWI76_RS00175 at 37.639 kb on + strand, within BWI76_RS00175 at 37.816 kb on + strand at 37.816 kb on + strand at 38.001 kb on - strand at 38.550 kb on - strand, within BWI76_RS00180 at 38.550 kb on - strand, within BWI76_RS00180
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1 remove 35,618 - -0.1 35,651 + +1.0 35,651 + -1.9 35,871 + -0.9 35,926 - +0.4 35,958 - -1.1 36,079 - BWI76_RS00175 0.10 +0.2 36,181 - BWI76_RS00175 0.15 -0.3 36,313 - BWI76_RS00175 0.21 +0.1 36,853 - BWI76_RS00175 0.46 -0.1 36,853 - BWI76_RS00175 0.46 -1.0 36,853 - BWI76_RS00175 0.46 -1.7 36,853 - BWI76_RS00175 0.46 +0.5 36,853 - BWI76_RS00175 0.46 -0.6 36,964 + BWI76_RS00175 0.51 -1.2 36,974 - +1.5 36,974 - BWI76_RS00175 0.51 -0.4 36,977 - BWI76_RS00175 0.52 -1.6 37,166 + BWI76_RS00175 0.60 -0.9 37,282 + BWI76_RS00175 0.66 -2.3 37,301 + BWI76_RS00175 0.67 -1.1 37,368 - BWI76_RS00175 0.70 -0.4 37,527 - BWI76_RS00175 0.77 -3.3 37,639 + BWI76_RS00175 0.82 +0.0 37,816 + -0.6 37,816 + -2.3 38,001 - -1.1 38,550 - BWI76_RS00180 0.25 +1.5 38,550 - BWI76_RS00180 0.25 -0.5
Or see this region's nucleotide sequence