Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00080

Experiment: KLEB20 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00070 and BWI76_RS00075 are separated by 50 nucleotidesBWI76_RS00075 and BWI76_RS00080 overlap by 4 nucleotidesBWI76_RS00080 and BWI76_RS00085 overlap by 4 nucleotidesBWI76_RS00085 and BWI76_RS00090 are separated by 169 nucleotides BWI76_RS00070: BWI76_RS00070 - MFS transporter, at 13,479 to 14,969 _RS00070 BWI76_RS00075: BWI76_RS00075 - N-acetylmannosamine-6-phosphate 2-epimerase, at 15,020 to 15,709 _RS00075 BWI76_RS00080: BWI76_RS00080 - N-acetylmannosamine kinase, at 15,706 to 16,581 _RS00080 BWI76_RS00085: BWI76_RS00085 - hypothetical protein, at 16,578 to 17,042 _RS00085 BWI76_RS00090: BWI76_RS00090 - multidrug transporter subunit MdtL, at 17,212 to 18,393 _RS00090 Position (kb) 15 16 17Strain fitness (log2 ratio) -3 -2 -1 0 1at 14.759 kb on + strand, within BWI76_RS00070at 14.884 kb on + strandat 14.922 kb on - strandat 14.925 kb on - strandat 14.925 kb on - strandat 15.018 kb on - strandat 15.089 kb on + strand, within BWI76_RS00075at 15.320 kb on - strand, within BWI76_RS00075at 15.361 kb on + strand, within BWI76_RS00075at 16.009 kb on + strand, within BWI76_RS00080at 16.225 kb on + strand, within BWI76_RS00080at 16.451 kb on + strand, within BWI76_RS00080at 16.461 kb on + strand, within BWI76_RS00080at 16.469 kb on - strand, within BWI76_RS00080at 16.514 kb on - strandat 16.529 kb on - strandat 16.601 kb on - strandat 16.609 kb on + strandat 16.616 kb on + strandat 16.631 kb on + strand, within BWI76_RS00085at 16.631 kb on + strand, within BWI76_RS00085at 16.932 kb on - strand, within BWI76_RS00085at 17.020 kb on - strandat 17.088 kb on - strandat 17.176 kb on + strandat 17.176 kb on + strandat 17.176 kb on + strandat 17.176 kb on + strandat 17.184 kb on - strandat 17.184 kb on - strandat 17.214 kb on - strandat 17.244 kb on + strandat 17.252 kb on - strandat 17.252 kb on - strandat 17.498 kb on - strand, within BWI76_RS00090at 17.539 kb on + strand, within BWI76_RS00090at 17.546 kb on + strand, within BWI76_RS00090

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1
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14,759 + BWI76_RS00070 0.86 +0.8
14,884 + -1.0
14,922 - -1.1
14,925 - -0.2
14,925 - -0.7
15,018 - +1.0
15,089 + BWI76_RS00075 0.10 +0.2
15,320 - BWI76_RS00075 0.43 +0.2
15,361 + BWI76_RS00075 0.49 -1.4
16,009 + BWI76_RS00080 0.35 -0.8
16,225 + BWI76_RS00080 0.59 -1.1
16,451 + BWI76_RS00080 0.85 -1.5
16,461 + BWI76_RS00080 0.86 -2.4
16,469 - BWI76_RS00080 0.87 -0.8
16,514 - -0.6
16,529 - +1.0
16,601 - -1.0
16,609 + +0.1
16,616 + -0.6
16,631 + BWI76_RS00085 0.11 -0.2
16,631 + BWI76_RS00085 0.11 -1.0
16,932 - BWI76_RS00085 0.76 +0.2
17,020 - -0.0
17,088 - +0.6
17,176 + -0.4
17,176 + +0.0
17,176 + -0.9
17,176 + -1.0
17,184 - +0.1
17,184 - +0.4
17,214 - +1.1
17,244 + -0.2
17,252 - +1.3
17,252 - +0.8
17,498 - BWI76_RS00090 0.24 -3.5
17,539 + BWI76_RS00090 0.28 +0.1
17,546 + BWI76_RS00090 0.28 -0.0

Or see this region's nucleotide sequence